Procyanidin B2
(Synonyms: 原花青素 B2; Proanthocyanidin B2) 目录号 : GN10599
Procyanidin B2 是一种天然黄酮类物质，具有抗肿瘤，抗氧化等作用。
Cas No.:29106-49-8
Sample solution is provided at 25 µL, 10mM.
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Procyanidin B2 (PB2), an antioxidant from the proanthocyanidin flavonoid polyphenol group, is found in plants like grape seeds, berries, cocoa, and tea, offering pharmacological benefits including anti-oxidative and anti-inflammatory effects [1-3].
Procyanidin B2, when administered at concentrations ranging from 0 to 10 μM for 24 h, suppresses the secretion of sFlt-1 from primary placental tissues andendothelial cells [4]. Additionally, at concentrations of 1 to 2.5 μM, Procyanidin B2 enhances the angiogenic function, survival, and migration of Endothelial Progenitor Cells (EPCs) exposed to high glucose (HG) conditions [5].
Procyanidin B2 (PB2), administered intravenously at doses of 0.5, 1, or 2 mg/kg, contributes to the treatment of sepsis-induced Acute Lung Injury (ALI) by regulating cytokine storms and mitigating inflammation through modulation of the TLR4/NF-κB, PI3K/Akt, Hippo, and Rho signaling pathways [6]. Furthermore, Procyanidin B2 (50 mg/kg/day) administered orally for 6 weeks attenuates liver injury and hepatocyte pyroptosis in mice exposed to nicotine [7].
References:
[1]. Chen J, Zhong K, et,al. Procyanidin B2: A promising multi-functional food-derived pigment for human diseases. Food Chem. 2023 Sep 15;420:136101. doi: 10.1016/j.foodchem.2023.136101. Epub 2023 Apr 7. Erratum in: Food Chem. 2024 Feb 1;433:137359. PMID: 37059021.
[2]. Zhang JQ, Gao BW, et,al. Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2. Oxid Med Cell Longev. 2016;2016:6147345. doi: 10.1155/2016/6147345. Epub 2016 Jan 19. PMID: 27057282; PMCID: PMC4745910.
[3]. Kopustinskiene DM, Savickas A, et,al. Direct effects of (-)-epicatechin and procyanidin B2 on the respiration of rat heart mitochondria. Biomed Res Int. 2015;2015:232836. doi: 10.1155/2015/232836. Epub 2015 Feb 24. PMID: 25811024; PMCID: PMC4354975.
[4]. Liu L, Wang R, et,al. Procyanidin B2 ameliorates endothelial dysfunction and impaired angiogenesis via the Nrf2/PPARγ/sFlt-1 axis in preeclampsia. Pharmacol Res. 2022 Mar;177:106127. doi: 10.1016/j.phrs.2022.106127. Epub 2022 Feb 10. PMID: 35150862.
[5]. Fan J, Liu H, et,al. Procyanidin B2 improves endothelial progenitor cell function and promotes wound healing in diabetic mice via activating Nrf2. J Cell Mol Med. 2021 Jan;25(2):652-665. doi: 10.1111/jcmm.16111. Epub 2020 Nov 20. PMID: 33215883; PMCID: PMC7812287.
[6]. Kim GO, Park DH, et,al. Procyanidin B2 Attenuates Sepsis-Induced Acute Lung Injury via Regulating Hippo/Rho/PI3K/NF-κB Signaling Pathway. Int J Mol Sci. 2023 Apr 27;24(9):7930. doi: 10.3390/ijms24097930. PMID: 37175637; PMCID: PMC10177954.
[7]. Liu J, Yao Q, et,al. Procyanidin B2 Attenuates Nicotine-Induced Hepatocyte Pyroptosis through a PPARγ-Dependent Mechanism. Nutrients. 2022 Apr22;14(9):1756. doi: 10.3390/nu14091756. PMID: 35565726; PMCID: PMC9103831.
Procyanidin B2 (PB2)是一种来自原花青素类黄酮多酚组的抗氧化剂，存在于葡萄籽、浆果、可可和茶等植物中，具有抗氧化和抗炎作用[1-3]。
Procyanidin B2在0 ~ 10 μM浓度范围内作用24小时，可抑制原代胎盘组织和内皮细胞分泌sFlt-1[4]。此外，在1 ~ 2.5 μM浓度下，Procyanidin B2可增强暴露于高糖(HG)条件下内皮祖细胞(EPCs)的血管生成功能、存活和迁移[5]。
Procyanidin B2 (PB2)以0.5-2 mg/kg的剂量静脉注射，通过调节TLR4/NF-κB、PI3K/Akt、Hippo和Rho信号通路，调节细胞因子风暴和减轻炎症，有助于治疗小鼠败血症诱导的急性肺损伤(ALI)[6]。此外，Procyanidin B2 (50 mg/kg/day)口服6周可减轻尼古丁暴露造成的小鼠肝损伤和肝细胞焦亡[7]。
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Cell experiment [1]: |
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Cell lines |
HUVECs( Human Umbilical Vein Endothelial Cells，HUVEC) |
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Preparation method |
HUVECs were treated with different concentrations of procyanidin B2 (0, 2.5, 5, and 10 μM) for 24 h. |
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Reaction Conditions |
0-10 μM; 24 h |
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Applications |
Procyanidin B2 inhibits sFlt-1 secretion from HUVEC in a concentration-dependent manner. |
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Animal experiment [2]: |
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Animal models |
Male C57BL/6 mice (27 g; 6–7 weeks old) |
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Preparation method |
LPS was injected into the mice via intraperitoneal injection at a dose of 5 mg/kg with 0.2% DMSO. One hour and 12 h after the LPS injection, the mice were given intravenous doses of Procyanidin B2 (0.5, 1, or 2 mg/kg). |
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Dosage form |
0.5, 1, or 2 mg/kg; i.v |
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Applications |
Procyanidin B2 Alleviated Acute Lung Injury Induced by Intraperitoneal LPS. |
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References: [1]. Liu L, Wang R, et,al. Procyanidin B2 ameliorates endothelial dysfunction and impaired angiogenesis via the Nrf2/PPARγ/sFlt-1 axis in preeclampsia. Pharmacol Res. 2022 Mar;177:106127. doi: 10.1016/j.phrs.2022.106127. Epub 2022 Feb 10. PMID: 35150862. [2]. Kim GO, Park DH, et,al.Procyanidin B2 Attenuates Sepsis-Induced Acute Lung Injury via Regulating Hippo/Rho/PI3K/NF-κB Signaling Pathway. Int J Mol Sci. 2023 Apr 27;24(9):7930. doi: 10.3390/ijms24097930. PMID: 37175637; PMCID: PMC10177954. |
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| Cas No. | 29106-49-8 | SDF | |
| 别名 | 原花青素 B2; Proanthocyanidin B2 | ||
| 化学名 | (2R,3R)-2-(3,4-dihydroxyphenyl)-8-[(2R,3R,4R)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-3,4-dihydro-2H-chromen-4-yl]-3,4-dihydro-2H-chromene-3,5,7-triol | ||
| Canonical SMILES | C1C(C(OC2=C1C(=CC(=C2C3C(C(OC4=CC(=CC(=C34)O)O)C5=CC(=C(C=C5)O)O)O)O)O)C6=CC(=C(C=C6)O)O)O | ||
| 分子式 | C30H26O12 | 分子量 | 578.52 |
| 溶解度 | DMF: 30 mg/mL,DMSO: 30 mg/mL,Ethanol: 30 mg/mL,PBS (pH 7.2): 10 mg/mL | 储存条件 | Store at -20℃ |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7285 mL | 8.6427 mL | 17.2855 mL |
| 5 mM | 0.3457 mL | 1.7285 mL | 3.4571 mL |
| 10 mM | 0.1729 mL | 0.8643 mL | 1.7285 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet