Prolactin-Releasing Peptide (1-31) (human)
(Synonyms: Human PrRP-31, PrRP(1-31)) 目录号 : GC18366A neuropeptide involved in regulating food intake
Cas No.:215510-22-8
Sample solution is provided at 25 µL, 10mM.
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- Purity: >99.50%
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Animal experiment: | Rats[1]Groups of rats are injected with either Prolactin Releasing Peptide (1-31) 5 nM or saline. Prolactin Releasing Peptide (1-31), human is dissolved in saline is administered in a total volume of 10 μL. Animals are habituated to the injection procedures by three ICV injections prior to the study to minimize stress in the animals. At 10, 20, 60 minutes following injection, rats are decapitated and trunk blood collected into plastic tubes[1]. |
References: [1]. Langmead CJ, et al. Characterization of the binding of [(125)I]-human prolactin releasing peptide (PrRP) to GPR10, a novel G protein coupled receptor. Characterization of the binding of [(125)I]-human prolactin releasing peptide (PrRP) to GPR10, a novel G protein coupled receptor. |
Prolactin releasing peptide (PrRP) is a 31-amino acid neuropeptide involved in regulating food intake. It is found in the hypothalamus, medulla, and pituitary in rats. PrRP is an agonist of the G-protein coupled receptors GPR10/hGR3 and neuropeptide FF receptor (hNPFF2; Kis = 1 and 19 nM, respectively). It stimulates calcium mobilization in HEK293 cells expressing GPR10 (EC50 = 1.5 nM) and radiolabeled GTPγS binding to membrane homogenates from CHO cells transfected with hNPFF2 (EC50 = 240 nM). PrRP (100 nM) increases the release of gonadotropin-releasing hormone , vasoactive intestinal peptide-, and galanin from rat medial basal hypothalamic explants. It increases plasma levels of luteinizing hormone, follicle stimulating hormone, and testosterone in male rats when administered at an intracerebroventricular dose of 5 nmol. PrRP reduces food intake in fasted rats to 58.4, 53.4, and 55.4% of control levels when administered at doses of 1, 5, and 10 nmol, respectively.
References:
[1]. Seal, L.G., Small, C.J., Shillo, W.S., et al. PRL-releasing peptide inhibits food intake in male rats via the dorsomedial hypothalamic nucleus and not the paraventricular hypothalamic nucleus Endocrinology 142(10), 4236-4243 (2001).
[2].Langmead, C.J., Szekeres, P.G., Chambers, J.K., et al. Characterization of the binding of [125I]-human prolactin releasing peptide (PrRP) to GPR10, a novel G protein coupled receptor Br. J. Pharmacol. 131(4), 683-688 (2000).
[3]. Engstr?m, M., Brandt, A., Wurster, S., et al. Prolactin releasing peptide has high affinity and efficacy at neuropeptide FF2 receptors J. Pharmacol. Exp. Ther. 305(3), 825-832 (2003).
[4].Seal, L.J., Small, C.J., Kim, M.S., et al. Prolactin releasing peptide (PrRP) stimulates luteinizing hormone (LH) and follicle stimulating hormone (FSH) via a hypothalamic mechanism in male rats Endocrinology 141(5), 1909-1912 (2000).
Cas No. | 215510-22-8 | SDF | |
别名 | Human PrRP-31, PrRP(1-31) | ||
化学名 | N/A | ||
Canonical SMILES | N/A | ||
分子式 | C160H252N56O42S | 分子量 | 3664.2 |
溶解度 | Water: 1 mg/ml | 储存条件 | Store at -20°C |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.2729 mL | 1.3646 mL | 2.7291 mL |
5 mM | 0.0546 mL | 0.2729 mL | 0.5458 mL |
10 mM | 0.0273 mL | 0.1365 mL | 0.2729 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
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1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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