Protease and Phosphatase Inhibitor Cocktail (EDTA plus, 100X in ddH2O)

This product is a ready-to-use mixture of protease and phosphatase inhibitors (EDTA plus, 100X in ddH2O), which can effectively inhibit the activity of proteases and phosphatases in routine cell lysis and protein extraction. This product is suitable for experiments such as protein extraction of mammalian cells, primary cells, yeast cells, bacteria, animal tissues and plant tissues.
The main ingredients of this product include: serine|cysteine protease inhibitor Leupeptin, bovine trypsin inhibitor Aprotinin, aminopeptidase inhibitor Bestatin, cysteine protease inhibitor E-64, serine|threonine phosphatase reversible inhibitor β-glycerophosphate, serine|threonine phosphatase irreversible inhibitor Sodium Pyrophosphate, acid phosphatase inhibitor Sodium Fluoride, alkaline phosphatase|tyrosine phosphatase inhibitor Sodium Orthovanadate, and a metalloproteinase inhibitor EDTA aqueous solution is also provided.
This product can effectively inhibit the function of proteases such as serine proteases, leucine aminopeptidases, cysteine proteases, metalloproteases and aminopeptidase B, as well as phosphatases such as serine|threonine phosphatases, protein tyrosine phosphatases, acid phosphatases and alkaline phosphatases.
Recommended use: When lysing cells or extracting proteins, mix this product and lysis buffer at a volume ratio of 1:100.
EDTA in the product components may interfere with IMAC (immobilized metal affinity chromatography), protein stability (such as metal-containing proteins) or subsequent determinations. If you need to purify metalloproteases, aspartic acid proteases and Poly-His-tagged fusion proteins, it is recommended to use an EDTA-free protease and phosphatase inhibitor cocktail (GK10015).

本产品是一种即用型蛋白酶和磷酸酶抑制剂混合物（Protease and Phosphatase Inhibitor Cocktail(EDTA plus, 100X in ddH2O)），可以有效抑制常规细胞裂解和蛋白质提取过程中的蛋白酶和磷酸酶活性。本产品适用于哺乳动物细胞、原代细胞、酵母细胞、细菌、动物组织及植物组织的蛋白提取等实验。
本产品的主要成分包括：丝氨酸|半胱氨酸蛋白酶抑制剂Leupeptin、牛胰蛋白酶抑制剂Aprotinin、氨肽酶抑制剂Bestatin、半胱氨酸蛋白酶抑制剂E-64、丝氨酸|苏氨酸磷酸酶可逆抑制剂β-glycerophosphate、丝氨酸|苏氨酸磷酸酶不可逆抑制剂Sodium Pyrophosphate、酸性磷酸酶抑制剂Sodium Fluoride、碱性磷酸酶|酪氨酸磷酸酶抑制因子Sodium Orthovanadate，另提供一支金属蛋白酶抑制剂EDTA水溶液。
本产品可有效抑制丝氨酸蛋白酶、亮氨酸氨基肽酶、半胱氨酸蛋白酶、金属蛋白酶和氨肽酶B等蛋白酶以及丝氨酸|苏氨酸磷酸酶、蛋白酪氨酸磷酸酶、酸性磷酸酶和碱性磷酸酶等磷酸酶的功能。
建议使用方法：在裂解细胞或提取蛋白时，将本产品和裂解液按照1:100的体积比混匀使用。
产品组分中的EDTA可能会干扰IMAC（固定化金属亲和色谱）、蛋白质稳定性（如含金属蛋白质）或后续测定。如需纯化金属蛋白酶、天冬氨酸蛋白酶及Poly-His标记的融合蛋白，建议使用不含EDTA的蛋白酶和磷酸酶抑制剂混合物（GK10015)。