Protease Inhibitor Cocktail (EDTA-Free,100× in DMSO)

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.

蛋白酶抑制剂混合物被用于细胞裂解液或组织提取物中，以增加蛋白质的稳定性。

内源性蛋白质在平衡状态下产生和清除，因此它们的细胞水平通常在稳定的环境条件下保持稳定。然而，在体外研究细胞时，蛋白质的产生大大减缓，降解加速。为了防止这种情况下蛋白质的降解，可以利用已知存在的酶抑制剂混合物来阻止其降解。

蛋白酶抑制剂混合物被用于细胞裂解液或组织提取物中，以增加蛋白质的稳定性。这种混合物的作用是抑制会降解磷酸化或非磷酸化蛋白底物的蛋白酶。

蛋白酶抑制剂混合物已经被优化并用于哺乳动物细胞和组织提取中进行测试。它包含对丝氨酸、半胱氨酸和酸性蛋白酶以及氨肽酶具有广泛特异性的抑制剂。这种混合物是作为DMSO溶液供应的，可以直接使用。