Protein-G His
目录号 : GP24242Protein G His Tag Recombinant
Sample solution is provided at 25 µL, 10mM.
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Protein G His Tag Recombinant produced in E.Coli is a 201 amino acids protein which contains amino acid 190-384 of the Streptococcus sp with a C-terminal 6-His tag, and having a molecular mass of 21.6kDa. But it migrates with an apparent molecular mass of 32kDa in SDS-PAGE.The Protein G His Tag is purified by proprietary chromatographic techniques.
| Purity | Greater than 95.0% as determined by SDS-PAGE and HPLC analyses. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Solubility | It is recommended to reconstitute the lyophilized Protein G in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles. | ||
| Formulation | Lyophilized with no additives. | ||
Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.