Protirelin (Synthetic thyrotropin-releasing factor)
(Synonyms: 普罗瑞林; Thyrotropin-releasing-hormone; TRH) 目录号 : GC30588
A tropic hormone
Cas No.:24305-27-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Thyrotropin-releasing Hormone (TRH) is a tropic hormone that stimulates release of thyroid-stimulating hormone (TSH) and prolactin.1 It binds to the human TRH receptor (TRHR) expressed in CHO cells and to rat TRHR in rat brain membranes (IC50s = 25 and 198 nM, respectively).2,3 TRH (1 μM) decreases polyphosphoinositide and increases arachidonic and oleic acid diacylglycerol incorporation into lipids of GH3 rat pituitary tumor cells.4 In vitro, it dose-dependently increases TSH release from rat anterior pituitary slices and prolactin release from cultured anterior pituitary cells.5,6 In vivo, TRH (0.01 mg/kg) increases TSH and prolactin plasma levels in rats.1
1.Dettmar, P.W., Lynn, A.G., Metcalf, G., et al.The ability of RX 77368 - a stabilised analogue of TRH - to provoke the secretion of prolactin and TSH in vivoNeuropeptides3(1)1-8(1982) 2.Yamada, M., Iwasaki, T., Satoh, T., et al.Activation of the thyrotropin-releasing hormone (TRH) receptor by a direct precursor of TRH, TRH-GlyNeurosci. Lett.196(1-2)109-112(1995) 3.Bhargava, H.N., and Das, S.Evidence for opiate action at the brain receptors for thyrotropin-releasing hormoneBrain Res.368(2)262-267(1986) 4.Martin, T.F.J.Thyrotropin-releasing hormone rapidly activates the phosphodiester hydrolysis of polyphosphoinositides in GH3 pituitary cells. Evidence for the role of a polyphosphoinositide-specific phospholipase C in hormone actionJ. Biol. Chem.258(24)14816-14822(1983) 5.Iriuchijima, T., Michimata, T., Miyashita, K., et al.Thyroid hormones regulate the formation of inositol phosphate in response to thyrotropin-releasing hormone in rat anterior pituitariesNeuropeptides21(1)49-53(1992) 6.Apfelbaum, M.E.Role of vasoactive intestinal peptide and 5-HT2 receptor subtype in serotonin stimulation of basal and thyrotropin-releasing-hormone-induced prolactin release in vitro from rat pituitary cellsNeuroendocrinology67(1)45-50(1998)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7595 mL | 13.7977 mL | 27.5953 mL |
| 5 mM | 0.5519 mL | 2.7595 mL | 5.5191 mL |
| 10 mM | 0.276 mL | 1.3798 mL | 2.7595 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。