Prunetin
(Synonyms: 樱黄素) 目录号 : GC46002An isoflavone with diverse biological activities
Cas No.:552-59-0
Sample solution is provided at 25 µL, 10mM.
Prunetin is an isoflavone that has been found in P. yedoensis and has diverse biological activities.1,2,3,4,5 It is an allosteric inhibitor of hamster liver aldehyde dehydrogenase 2 (ALDH2; IC50 = 0.45 μM) and an antagonist of the progesterone receptor when used at concentrations of 25 and 50 μM.2,3 It has estrogenic activity in MVLN cells when used at concentrations ranging from 1 to 50 μM and inhibits proliferation of MCF-7 breast cancer cells when used at 0.01 to 50 μM.4 It decreases LPS-induced increases in nitric oxide (NO) and prostaglandin E2 levels, NOS2/iNOS expression, and NF-κB activation in RAW 264.7 macrophages when used at concentrations of 50 and 100 μM.1 Prunetin (10 mg/kg) prevents LPS-induced increases in serum TNF-α, IL-1β, and IL-6 levels in a mouse model of septic shock. It also inhibits the secretion of matrix metalloproteinase-3 (MMP-3) in isolated rabbit articular chondrocytes and prevents the production of MMP-3 in the knee joint of rats in a model of osteoarthritis following administration of a 50 or 100 μM dose into the knee joint.5
|1. Gabsik, Y., Ham, I., and Choi, H.-Y. Anti-inflammatory effect of prunetin via the suppression of NF-κB pathway. Food Chem. Toxicol. 58, 124-132 (2013).|2. Lowe, E.D., Gao, G.-Y., Johnson, L.N., et al. Structure of daidzin, a naturally occurring anti-alcohol-addiction agent, in complex with human mitochondrial aldehyde dehydrogenase. J. Med. Chem. 51(15), 4482-4487 (2008).|3. Lee, J.-H., Dean, M., Austin, J.R., et al. Irilone from red clover (Trifolium pratense) potentiates progesterone signaling. J. Nat. Prod. 81(9), 1962-1967 (2018).|4. Le Bail, J.-C., Champavier, Y., Chulia, A.-J., et al. Effects of phytoestrogens on aromatase, 3β and 17β-hydroxysteroid dehydrogenase activities and human breast cancer cells. Life Sci. 66(14), 1281-1291 (2000).|5. Nam, D.C., Kim, B.K., Lee, H.J., et al. Effects of prunetin on the proteolytic activity, secretion and gene expression of MMP-3 in vitro and production of MMP-3 in vivo. Korean J. Physiol. Pharmacol. 20(2), 221-228 (2016).
Cas No. | 552-59-0 | SDF | |
别名 | 樱黄素 | ||
Canonical SMILES | COC1=CC(O)=C2C(OC=C(C3=CC=C(O)C=C3)C2=O)=C1 | ||
分子式 | C16H12O5 | 分子量 | 284.3 |
溶解度 | DMF: 10 mg/ml,DMSO: 10 mg/ml,DMSO:PBS (pH 7.2) (1:3): 0.25 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.5174 mL | 17.5871 mL | 35.1741 mL |
5 mM | 0.7035 mL | 3.5174 mL | 7.0348 mL |
10 mM | 0.3517 mL | 1.7587 mL | 3.5174 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet