Putrescine
(Synonyms: 四亚甲基二胺,Putrescine; NSC 60545; Putramine) 目录号 : GC19608
Putrescine腐胺(多胺、亚精胺和精胺)是一种低分子量的有机阳离子，它们在细胞生长、细胞分化、调节酶活性、调节离子通道以及控制DNA和蛋白质合成等方面具有重要作用。
Cas No.:110-60-1
Sample solution is provided at 25 µL, 10mM.
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Putrescine (polyamines, spermidine and spermine) are organic cations of low molecular weight, it have important roles in cellular growth, cell differentiation, modulation of enzyme activities, regulation of ion channels and in the control of DNA and protein synthesis[1-2].
Putrescine(100 ng/ml) promoted induction of immature bone marrow derived DCs (BMDCs), along with the increased phagocytosis and migration, and altered cytokine secretion in immature BMDCs [3]. Putrescine(0.5mM) improved the mitochondrial function of both oocyte and CCs via antioxygenation and the regulation of glucose metabolism [4-5]. Putrescine(100 μM) shifts L-arginine-metabolism related-genes on BALB/c macrophages and affects infection by L. amazonensis[6].
Putrescine produces antidepressant-like effects. Putrescine (1-10 mg/kg, i.p) significantly reduced the immobility time both in the mouse forced swimming test (FST) and in the tail suspension test (TST), two models predictive of antidepressant activity [7]. Putrescine(100 μM;24h) elicits ROS-dependent activation of the salicylic acid pathway in Arabidopsis thaliana[8].
References:
[1]. Park MH, Igarashi K. Polyamines and their metabolites as diagnostic markers of human diseases. Biomol Ther (Seoul). 2013 Jan;21(1):1-9. doi: 10.4062/biomolther.2012.097. PMID: 24009852; PMCID: PMC3762300.
[2]. Bird J, Mohd-Hidir S,et,al. Putrescine--a potent endogenous anti-inflammatory substance in inflammatory exudates. Agents Actions. 1983 Jun;13(4):342-7. doi: 10.1007/BF01971486. PMID: 6613748.
[3]. Huang P, Wang M, et,al. Putrescine accelerates the differentiation of bone marrow derived dendritic cells via inhibiting phosphorylation of STAT3 at Tyr705. Int Immunopharmacol. 2023 Mar;116:109739. doi: 10.1016/j.intimp.2023.109739. Epub 2023 Jan 26. PMID: 36706590.
[4]. Xu W, Li L, et,al. Putrescine delays postovulatory aging of mouse oocytes by upregulating PDK4 expression and improving mitochondrial activity. Aging (Albany NY). 2018 Dec 16;10(12):4093-4106. doi: 10.18632/aging.101699. PMID: 30554191; PMCID: PMC6326651.
[5]. Tao Y, Tartia A, et,al. Can peri-ovulatory putrescine supplementation improve egg quality in older infertile women? J Assist Reprod Genet. 2019 Mar;36(3):395-402. doi: 10.1007/s10815-018-1327-x. Epub 2018 Nov 22. PMID: 30467617; PMCID: PMC6439130.
[6]. Zanatta JM, Acuña SM, et,al. Putrescine supplementation shifts macrophage L-arginine metabolism related-genes reducing Leishmania amazonensis infection. PLoS One. 2023 Mar 31;18(3):e0283696. doi: 10.1371/journal.pone.0283696. PMID: 37000792; PMCID: PMC10065296.
[7]. Zomkowski AD, Santos AR, et,al. Putrescine produces antidepressant-like effects in the forced swimming test and in the tail suspension test in mice. Prog Neuropsychopharmacol Biol Psychiatry. 2006 Dec 30;30(8):1419-25. doi: 10.1016/j.pnpbp.2006.05.016. Epub 2006 Jul 5. PMID: 16822602.
[8]. Liu C, Atanasov KE, et,al. Putrescine elicits ROS-dependent activation of the salicylic acid pathway in Arabidopsis thaliana. Plant Cell Environ. 2020 Nov;43(11):2755-2768. doi: 10.1111/pce.13874. Epub 2020 Sep 14. PMID: 32839979.
Putrescine腐胺(多胺、亚精胺和精胺)是一种低分子量的有机阳离子，它们在细胞生长、细胞分化、调节酶活性、调节离子通道以及控制DNA和蛋白质合成等方面具有重要作用[1-2]。
Putrescine (100 ng/ml)促进未成熟骨髓源性DC (BMDCs)的诱导，同时增加未成熟骨髓源性DC的吞噬和迁移，改变未成熟骨髓源性DC的细胞因子分泌[3]。Putrescine (0.5mM)通过抗氧化和调节葡萄糖代谢改善卵母细胞和CCs的线粒体功能[4-5]。Putrescine (100 μM)改变BALB/c巨噬细胞上l -精氨酸代谢相关基因，影响亚马逊乳杆菌感染[6]。
Putrescine产生类似抗抑郁的作用。Putrescine (1-10 mg/kg, i.p)在小鼠强迫游泳试验(FST)和悬尾试验(TST) (两种预测抗抑郁的模型)中显著减少静止时间 [7]。Putrescine (100 μM;24h)可诱导拟南芥中水杨酸通路的ROS依赖性激活[8]。
| Cell experiment [1]: | |
Cell lines | Bone marrow-derived murine dendritic cells |
Preparation Method | Cells were treated with putrescine and transwell experiments were performed. |
Reaction Conditions | 100 ng/ml |
Applications | Putrescine enhanced immature migration of DCs. Putrescine promoted the induction of bone marrow cells to immature DCs. |
| Animal experiment [2]: | |
Animal models | Swiss mice |
Preparation Method | Putrescine injected mice intraperitoneally and then observed and measured forced swimming test (FST) . The forced swimming test was conducted on mice, each of which was placed in an open cylindrical container to swim. A mouse was considered immobile when it stopped struggling and floated passively in the water, making only minimal movements to keep its head above the surface. |
Dosage form | 1–10 mg/kg; i.p; administered 30 min before the test |
Applications | Putrescine significantly decreased the immobility time in the forced swimming test (FST). |
References: | |
| Cas No. | 110-60-1 | SDF | |
| 别名 | 四亚甲基二胺,Putrescine; NSC 60545; Putramine | ||
| Canonical SMILES | NCCCCN | ||
| 分子式 | C4H12N2 | 分子量 | 88.15 |
| 溶解度 | >139.9 mg/mL in DMSO; >42.3 mg/mL in EtOH; >9.3 mg/mL in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 11.3443 mL | 56.7215 mL | 113.443 mL |
| 5 mM | 2.2689 mL | 11.3443 mL | 22.6886 mL |
| 10 mM | 1.1344 mL | 5.6721 mL | 11.3443 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet