Pyranine (HPTS)
(Synonyms: 溶剂绿7; HPTS; Solvent Green 7) 目录号 : GC30145
Pyranine (HPTS)（8-Hydroxypyrene-1,3,6-trisulfonic acid）是一种高水溶性、膜不渗透性的pH荧光指示剂，在水性缓冲液中的pKa约为7.3。Pyranine (HPTS)最大激发波长为405nm/450nm，最大发射波长为510nm。
Cas No.:6358-69-6
Sample solution is provided at 25 µL, 10mM.
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Pyranine (HPTS) (8-Hydroxypyrene-1,3,6-trisulfonic acid) is a highly water-soluble, membrane-impermeable pH fluorescent indicator with a pKa of approximately 7.3 in aqueous buffer[1]. The maximum excitation wavelength of Pyranine (HPTS) is 405nm/450nm, and the maximum emission wavelength is 510nm[2]. Pyranine (HPTS) has different fluorescence spectra in the protonated and deprotonated forms[3]. The excited state of Pyranine (HPTS) is much more acidic than the ground state, and can be used as an excited state proton transfer probe in solution[4]. Pyranine (HPTS) can be used as a new type of Cu+ fluorescent chemotaxis sensor, and its sensing properties are specific only to neutral pH[5]. Pyranine (HPTS) is sensitive to the fluorescence quenching of viologen, which can be reversed in the presence of glucose or other monosaccharides, and can be used as a glucose and carbohydrate sensor[6].
References:
[1] Lemos S. Acoustic" Wall-Less" Test Tubes for Cell Biology in Type 2 Diabetes Related Research[D]. Universidade da Beira Interior (Portugal), 2008.
[2] Chowdhury R, Saha A, Mandal A K, et al. Excited state proton transfer in the lysosome of live lung cells: normal and cancer cells[J]. The Journal of Physical Chemistry B, 2015, 119(6): 2149-2156.
[3] de Nooijer L J, Toyofuku T, Oguri K, et al. Intracellular pH distribution in foraminifera determined by the fluorescent probe HPTS[J]. Limnology and Oceanography: Methods, 2008, 6(11): 610-618.
[4] Wang Y, Fang C. Femtosecond Stimulated Raman Spectroscopy (FSRS) Investigation of Excited-State Hydrogen-Bonding Dynamics and Photoacidity in Solution[M]//Hydrogen-Bonding Research in Photochemistry, Photobiology, and Optoelectronic Materials. 2019: 323-367.
[5] Saha T, Sengupta A, Hazra P, et al. In vitro sensing of Cu+ through a green fluorescence rise of pyranine[J]. Photochemical & Photobiological Sciences, 2014, 13: 1427-1433.
[6] Cappuccio F E, Suri J T, Cordes D B, et al. Evaluation of pyranine derivatives in boronic acid based saccharide sensing: Significance of charge interaction between dye and quencher in solution and hydrogel[J]. Journal of Fluorescence, 2004, 14: 521-533.
Pyranine (HPTS)（8-Hydroxypyrene-1,3,6-trisulfonic acid）是一种高水溶性、膜不渗透性的pH荧光指示剂，在水性缓冲液中的pKa约为7.3[1]。Pyranine (HPTS)最大激发波长为405nm/450nm，最大发射波长为510nm[2]。Pyranine (HPTS)在质子化和去质子化形式下具有不同的荧光光谱[3]。Pyranine (HPTS)的激发态比基态酸性大得多，能够用作溶液中激发态质子转移探针[4]。Pyranine (HPTS)能够作为一类新型的Cu+荧光趋化传感器，其传感特性仅特定于中性pH值[5]。Pyranine (HPTS)对紫精的荧光猝灭敏感，在葡萄糖或其他单糖存在下可以逆转，能够用作葡萄糖和碳水化合物传感器[6]。
| Cas No. | 6358-69-6 | SDF | |
| 别名 | 溶剂绿7; HPTS; Solvent Green 7 | ||
| Canonical SMILES | O=S(C1=C(C2=C34)C=CC4=C(O)C=C(S(=O)([O-])=O)C3=CC=C2C(S(=O)([O-])=O)=C1)([O-])=O.[Na+].[Na+].[Na+] | ||
| 分子式 | C16H7Na3O10S3 | 分子量 | 524.39 |
| 溶解度 | Water: 100 mg/mL (190.70 mM; Need ultrasonic);DMSO: 25 mg/mL (47.67 mM; Need ultrasonic) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.907 mL | 9.5349 mL | 19.0698 mL |
| 5 mM | 0.3814 mL | 1.907 mL | 3.814 mL |
| 10 mM | 0.1907 mL | 0.9535 mL | 1.907 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet