Pyrogallol
(Synonyms: 邻苯三酚) 目录号 : GC32391
A natural oxidant
Cas No.:87-66-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Pyrogallol is a natural oxidant that can generate superoxide (O2-) in alkaline solutions through autoxidation to a semiquinone radical.1 Importantly, the semiquinone radical can react with O2- in an acidic environment to produce a quinone and H2O2.1 Pyrogallol autoxidation is used in superoxide dismutase activity assays.1 It can also be used in assays to assess antioxidant capacity.2,3 Pyrogallol is used in some biological systems as an O2- scavenger.4 In other biological systems, it is used as an O2- generator.5,6 Pyrogallol effectively scavenges DPPH radical and ABTS+ in vitro.7 Pyrogallol is a product of tannin degradation to gallic acid by ruminant microbes and has hepatotoxic and nephrotoxic effects in vivo.8
1.Magnani, L., Gaydou, E.M., and Hubaud, J.C.Spectrophotometric measurement of antioxidant properties of flavones and flavonols against superoxide anionAnal. Chim. Acta411209-216(2000) 2.Li, X.Improved pyrogallol autoxidation method: A reliable and cheap superoxide-scavenging assay suitable for all antioxidantsJ. Agric. Food. Chem.60(25)6418-6424(2012) 3.Chang, C.S., Chang, C.L., and Lai, G.H.Reactive oxygen species scavenging activities in a chemiluminescence model and neuroprotection in rat pheochromocytoma cells by astaxanthin,βcarotene, and canthaxanthinKaohsiung J. Med. Sci.29(8)412-421(2013) 4.Wang.T., Lin, H., Tu, Q., et al.Fisetin protects DNA against oxidative damage and its possible mechanismAdv. Pharm. Bull.6(2)267-270(2016) 5.Sindler, A.L., Reyes, R., Chen, B., et al.Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arteriolesJ. Appl. Physiol.114(5)681-693(2013) 6.Moreno-Manzano, V., Ishikawa, Y., Lucio-Cazana, J., et al.Selective involvement of superoxide anion, but not downstream compounds hydrogen peroxide and peroxynitrite, in tumor necrosis factor-α-induced apoptosis of rat mesangial cellsJ. Biol. Chem.275(17)12684-12691(2000) 7.Mathew, S., Abraham, T.E., and Zakaria, Z.A.Reactivity of phenolic compounds towards free radicals under in vitro conditionsJ. Food. Sci. Technol.52(9)5790-5798(2015) 8.Reed, J.D.Nutritional toxicology of tannins and related polyphenols in forage legumesJ. Anim. Sci.73(5)1516-1528(1995)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 7.9296 mL | 39.6479 mL | 79.2959 mL |
| 5 mM | 1.5859 mL | 7.9296 mL | 15.8592 mL |
| 10 mM | 0.793 mL | 3.9648 mL | 7.9296 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。