Pyropheophorbide a methyl ester
(Synonyms: 焦脱镁叶绿酸Α甲酯,Pyropheophorbide-a methyl ester) 目录号 : GC49860
A photosensitizer
Cas No.:6453-67-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Pyropheophorbide a methyl ester is a photosensitizer and a derivative of chlorophyll a .1 It induces cell death in RIF-1 cells when used in combination with photodynamic therapy (PDT) at concentrations of 0.06 and 1 µM. Pyropheophorbide a methyl ester localizes to cell membranes and induces IL-1 receptor internalization and NF-κB activation following light exposure in HCT116 cells.2
1.Chen, Y., Zheng, X., Dobhal, M.P., et al.Methyl pyropheophorbide-a analogues: Potential fluorescent probes for the peripheral-type benzodiazepine receptor. Effect of central metal in photosensitizing efficacyJ. Med. Chem.48(11)3692-3695(2005) 2.Matroule, J.-Y., Bonizzi, G., MorliÈre, P., et al.Pyropheophorbide-a methyl ester-mediated photosensitization activates transcription factor NF-κB through the interleukin-1 receptor-dependent signaling pathwayJ. Biol. Chem.274(5)2988-3000(1999)
| Cas No. | 6453-67-4 | SDF | Download SDF |
| 别名 | 焦脱镁叶绿酸Α甲酯,Pyropheophorbide-a methyl ester | ||
| Canonical SMILES | O=C1CC(C2=C1C(C)=C(C=C3C(CC)=C(C)C(C=C4C(C=C)=C(C)C(N4)=C5)=N3)N2)=C6[C@@H](CCC(OC)=O)[C@H](C)C5=N6 | ||
| 分子式 | C34H36N4O3 | 分子量 | 548.7 |
| 溶解度 | Chloroform: slightly soluble,Methanol: slightly soluble when heated | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8225 mL | 9.1124 mL | 18.2249 mL |
| 5 mM | 0.3645 mL | 1.8225 mL | 3.645 mL |
| 10 mM | 0.1822 mL | 0.9112 mL | 1.8225 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Isolation of pyropheophorbide a from the leaves of Atalantia monophylla (ROXB.) CORR. (Rutaceae) as a possible antiviral active principle against herpes simplex virus type 2
Chem Pharm Bull (Tokyo) 1996 Jul;44(7):1415-7.PMID:8706147DOI:10.1248/cpb.44.1415.
Antiviral activity-guided isolation studies on the leaves of Atalantia monophylla (ROXB.) CORR. (Rutaceae) led to the identification of pyropheophorbide a (1), a simple chlorin derivative, from the chloroform extract (fr. B) as a possible antiviral active principle against herpes simplex virus type 2 (HSV-2). Pyropheophorbide a methyl ester (2) was also isolated from the hexane extract (fr. A).
Pheophorbide a Derivatives Exert Antiwrinkle Effects on UVB-Induced Skin Aging in Human Fibroblasts
Life (Basel) 2021 Feb 15;11(2):147.PMID:33671886DOI:10.3390/life11020147.
Pheophorbide a is a chlorophyll metabolic breakdown product. This study investigated the antiwrinkle effect of pheophorbide a (PA) and its derivatives, including pyropheophorbide a (PyroPA) and Pyropheophorbide a methyl ester (PyroPA-ME), on ultraviolet (UV) B-stimulated CCD-986sk fibroblasts. PA, PyroPA, and PyroPA-ME effectively suppressed reactive oxygen species accumulation in UVB-exposed CCD-986sk fibroblasts. All three pheophorbides also reduced UVB-induced matrix metalloproteinase (MMP)-1 secretion and mRNA expression of MMP-1, MMP-2, and MMP-9. Treatment with pheophorbides resulted in increased procollagen synthesis, and this required enhancement of procollagen type I C-peptide content and mRNA expression of collagen type I alpha 1 (COL1A1) and COL1A2 in CCD-986sk cells. These antiwrinkle effects were more potent with PA and PyroPA than with PyroPA-ME. Furthermore, PA and PyroPA suppressed UVB-induced phosphorylation of extracellular signal-regulated protein kinase and c-Jun N-terminal kinase but not p38. Moreover, all three pheophorbides inhibited NF-κB p65 phosphorylation. Therefore, these pheophorbides, especially PA and PyroPA, can be used as antiwrinkle agents, and PA- or PyroPA-rich natural resources can be used in functional cosmetics.