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Pyrrolidinedithiocarbamate ammonium Sale

(Synonyms: 吡咯烷二硫代氨基甲酸铵; Ammonium pyrrolidinedithiocarbamate; PDTC ammonium; APDC) 目录号 : GC13575

A potent and reversible NF-κB inhibitor with antioxidant properties

Pyrrolidinedithiocarbamate ammonium Chemical Structure

Cas No.:5108-96-3

规格 价格 库存 购买数量
100mg
¥350.00
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Sample solution is provided at 25 µL, 10mM.

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实验参考方法

Cell experiment:

The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy’s 5A medium supplemented with 10% fetal bovine serum. To study the effect of pyrrolidinedithiocarbamate ammonium on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1β for 18 h. Various concentrations (3-1000 μM) of pyrrolidinedithiocarbamate or its vehicle (culture medium) are added to the cells 30 min prior to IL-1β stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay[1].

Animal experiment:

Animal Administration: [2]Pyrrolidinedithiocarbamate is administered intraperitoneally to mice at dose levels of 100 and 50 mg/kg. Mice are divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+pyrrolidinedithiocarbamate-treated groupI (low-dose group), and DSS+pyrrolidinedithiocarbamate-treated groupII (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue are measured[2].

References:

[1]. Németh ZH, et al. Pyrrolidinedithiocarbamate inhibits NF-kappaB activation and IL-8 production in intestinal epithelial cells. Immunol Lett. 2003 Jan 2;85(1):41-6.
[2]. Qin JD, et al. Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury. Pharm Biol. 2014 Nov;52(11):1460-1466.

产品描述

Pyrrolidinedithiocarbamate ammonium is a selective NF-κB inhibitor.
Pretreatment of cells with pyrrolidinedithiocarbamate (3-1000 μM) dose-dependently attenuate IL-8 production. Furthermore, pyrrolidinedithiocarbamate (100 μM) suppresses the accumulation of IL-8 mRNA. Pyrrolidinedithiocarbamate inhibits the activation of NF-κB, because pyrrolidinedithiocarbamate suppresses both NF-κB DNA binding and NF-κB-dependent transcriptional activity. NF-κB inhibition with pyrrolidinedithiocarbamate decrease IL-8 production by intestinal epithelial cells[1].
The DSS+pyrrolidinedithiocarbamate ammonium-treated groupII exhibits suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+pyrrolidinedithiocarbamate ammonium-treated groupII. These findings suggest that suppression of NF-κB activity by pyrrolidinedithiocarbamate ammonium can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. pyrrolidinedithiocarbamate ammonium is useful for the treatment of ulcerative colitis[2].
Reference:
[1]. Németh ZH, et al. Pyrrolidinedithiocarbamate inhibits NF-kappaB activation and IL-8 production in intestinal epithelial cells. Immunol Lett. 2003 Jan 2;85(1):41-6.
[2]. Qin JD, et al. Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury. Pharm Biol. 2014 Nov;52(11):1460-1466.

Chemical Properties

Cas No. 5108-96-3 SDF
别名 吡咯烷二硫代氨基甲酸铵; Ammonium pyrrolidinedithiocarbamate; PDTC ammonium; APDC
化学名 ammonium pyrrolidine-1-carbodithioate
Canonical SMILES [S-]C(N1CCCC1)=S.[NH4+]
分子式 C5H12N2S2 分子量 164.28
溶解度 ≥50mg/mL in Water, ≥ 7.3mg/mL in DMSO, ≥ 12.975mg/mL in EtOH with gentle warming 储存条件 Store at 2-8°C
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溶解性数据

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1 mM 6.0872 mL 30.4358 mL 60.8717 mL
5 mM 1.2174 mL 6.0872 mL 12.1743 mL
10 mM 0.6087 mL 3.0436 mL 6.0872 mL
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