QS-21
(Synonyms: 皂苷QA21V1,Stimulon) 目录号 : GC63377
QS-21是一种免疫刺激性皂苷,可用作有效的疫苗佐剂。
Cas No.:141256-04-4
Sample solution is provided at 25 µL, 10mM.
QS-21 is an immune-stimulatory saponin that can be used as an effective vaccine adjuvant[1]. QS-21 enhances vaccine immune responses by activating dendritic cells and helper T cells, promoting antibody production and cell-mediated immune responses[2]. QS-21 is an activator of the NLRP3 inflammasome and leads to subsequent release of caspase-1-dependent pro-inflammatory cytokines IL-1β and IL-18[3].
In vitro, QS-21 (2.5, 5, 25 μM) treatment of HUVEC and PBMC cells for 24 hours significantly reduced cell viability, with this cytotoxic effect being more pronounced in HUVEC cells[4]. QS-21 (0-50 μM) treatment of two human gastric cancer cell lines (SNU 1 and KATO III) and a normal human cell line (GES-1) for 24 hours resulted in dose-dependent toxicity in KATO III and SNU 1 cells, with IC50 values of 67μM and 13.6μM, respectively, but showed almost no cytotoxicity to GES-1 cells[5].
In vivo, immunization of mice with Aβ42 formulated with QS-21 adjuvant produced the highest antibody titers, followed by CFA/IFA > alum > TMG[6].
References:
[1] Marty-Roix R, Vladimer G I, Pouliot K, et al. Identification of QS-21 as an inflammasome-activating molecular component of saponin adjuvants[J]. Journal of Biological Chemistry, 2016, 291(3): 1123-1136.
[2] Welsby I, Detienne S, N’Kuli F, et al. Lysosome-dependent activation of human dendritic cells by the vaccine adjuvant QS-21[J]. Frontiers in immunology, 2017, 7: 663.
[3] Lacaille-Dubois M A. Updated insights into the mechanism of action and clinical profile of the immunoadjuvant QS-21: A review[J]. Phytomedicine, 2019, 60: 152905.
[4] Campos-Estrada C, Riquelme B, Vergara M, et al. In vitro Notch-mediated adjuvant immunogenic potency is induced by combining QS-21 and MPL in a co-culture model of PBMC and HUVEC cells[J]. Toxicology in Vitro, 2020, 68: 104947.
[5] Guzmán L, Villalón K, Marchant M J, et al. In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents[J]. Scientific Reports, 2020, 10(1): 10534.
Cribbs D H, Ghochikyan A, Vasilevko V, et al. Adjuvant‐dependent modulation of Th1 and Th2 responses to immunization with β‐amyloid[J]. International immunology, 2003, 15(4): 505-514.
QS-21是一种免疫刺激性皂苷,可用作有效的疫苗佐剂[1]。QS-21的作用是增强疫苗的免疫反应,它通过激活树突状细胞和辅助T细胞,促进抗体生成和细胞介导的免疫反应[2]。QS-21是NLRP3炎症小体的激活剂,并导致caspase-1依赖性促炎细胞因子I1-1β / I1-18的后续释放[3]。
在体外,QS-21(2.5, 5, 25μM)处理HUVEC、PBMC细胞24 h,显著降低了细胞存活率,这种细胞毒性作用在HUVEC细胞中更为明显[4]。QS-21(0-50μM)处理两种人胃癌细胞系(SNU 1和KATO III)和人正常细胞系(GES-1)24h,以剂量依赖性方式对KATO III和SNU 1细胞产生毒性作用,IC50 值分别为67µM和13.6µM,但是对GES-1细胞几乎没有细胞毒性[5]。
在体内,使用QS-21佐剂配制的Aβ42对小鼠进行免疫加强,产生的抗体效价最高,随后是CFA/IFA >明矾> TMG[6]。
Cas No. | 141256-04-4 | SDF | |
别名 | 皂苷QA21V1,Stimulon | ||
分子式 | C92H148O46 | 分子量 | 1990.13 |
溶解度 | DMSO : 100 mg/mL (50.25 mM; Need ultrasonic); H2O : 50 mg/mL (25.12 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
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1 mg | 5 mg | 10 mg |
1 mM | 0.5025 mL | 2.5124 mL | 5.0248 mL |
5 mM | 0.1005 mL | 0.5025 mL | 1.005 mL |
10 mM | 0.0502 mL | 0.2512 mL | 0.5025 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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