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QS-21 Sale

(Synonyms: 皂苷QA21V1,Stimulon) 目录号 : GC63377

QS-21是一种免疫刺激性皂苷,可用作有效的疫苗佐剂。

QS-21 Chemical Structure

Cas No.:141256-04-4

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1mg
¥1,500.00
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5mg
¥4,410.00
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10mg
¥7,020.00
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Sample solution is provided at 25 µL, 10mM.

Description

QS-21 is an immune-stimulatory saponin that can be used as an effective vaccine adjuvant[1]. QS-21 enhances vaccine immune responses by activating dendritic cells and helper T cells, promoting antibody production and cell-mediated immune responses[2]. QS-21 is an activator of the NLRP3 inflammasome and leads to subsequent release of caspase-1-dependent pro-inflammatory cytokines IL-1β and IL-18[3].

In vitro, QS-21 (2.5, 5, 25 μM) treatment of HUVEC and PBMC cells for 24 hours significantly reduced cell viability, with this cytotoxic effect being more pronounced in HUVEC cells[4]. QS-21 (0-50 μM) treatment of two human gastric cancer cell lines (SNU 1 and KATO III) and a normal human cell line (GES-1) for 24 hours resulted in dose-dependent toxicity in KATO III and SNU 1 cells, with IC50 values of 67μM and 13.6μM, respectively, but showed almost no cytotoxicity to GES-1 cells[5].

In vivo, immunization of mice with Aβ42 formulated with QS-21 adjuvant produced the highest antibody titers, followed by CFA/IFA > alum > TMG[6].

References:
[1] Marty-Roix R, Vladimer G I, Pouliot K, et al. Identification of QS-21 as an inflammasome-activating molecular component of saponin adjuvants[J]. Journal of Biological Chemistry, 2016, 291(3): 1123-1136.
[2] Welsby I, Detienne S, N’Kuli F, et al. Lysosome-dependent activation of human dendritic cells by the vaccine adjuvant QS-21[J]. Frontiers in immunology, 2017, 7: 663.
[3] Lacaille-Dubois M A. Updated insights into the mechanism of action and clinical profile of the immunoadjuvant QS-21: A review[J]. Phytomedicine, 2019, 60: 152905.
[4] Campos-Estrada C, Riquelme B, Vergara M, et al. In vitro Notch-mediated adjuvant immunogenic potency is induced by combining QS-21 and MPL in a co-culture model of PBMC and HUVEC cells[J]. Toxicology in Vitro, 2020, 68: 104947.
[5] Guzmán L, Villalón K, Marchant M J, et al. In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents[J]. Scientific Reports, 2020, 10(1): 10534.
Cribbs D H, Ghochikyan A, Vasilevko V, et al. Adjuvant‐dependent modulation of Th1 and Th2 responses to immunization with β‐amyloid[J]. International immunology, 2003, 15(4): 505-514.

QS-21是一种免疫刺激性皂苷,可用作有效的疫苗佐剂[1]。QS-21的作用是增强疫苗的免疫反应,它通过激活树突状细胞和辅助T细胞,促进抗体生成和细胞介导的免疫反应[2]。QS-21是NLRP3炎症小体的激活剂,并导致caspase-1依赖性促炎细胞因子I1-1β / I1-18的后续释放[3]

在体外,QS-21(2.5, 5, 25μM)处理HUVEC、PBMC细胞24 h,显著降低了细胞存活率,这种细胞毒性作用在HUVEC细胞中更为明显[4]。QS-21(0-50μM)处理两种人胃癌细胞系(SNU 1和KATO III)和人正常细胞系(GES-1)24h,以剂量依赖性方式对KATO III和SNU 1细胞产生毒性作用,IC50 值分别为67µM和13.6µM,但是对GES-1细胞几乎没有细胞毒性[5]

在体内,使用QS-21佐剂配制的Aβ42对小鼠进行免疫加强,产生的抗体效价最高,随后是CFA/IFA >明矾> TMG[6]

化学性质

Cas No. 141256-04-4 SDF
别名 皂苷QA21V1,Stimulon
分子式 C92H148O46 分子量 1990.13
溶解度 DMSO : 100 mg/mL (50.25 mM; Need ultrasonic); H2O : 50 mg/mL (25.12 mM; Need ultrasonic) 储存条件 Store at -20°C
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1 mg 5 mg 10 mg
1 mM 0.5025 mL 2.5124 mL 5.0248 mL
5 mM 0.1005 mL 0.5025 mL 1.005 mL
10 mM 0.0502 mL 0.2512 mL 0.5025 mL
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