Quinacrine mustard (hydrochloride)
(Synonyms: 氮芥喹丫因) 目录号 : GC46210A fluorescent DNA-intercalating agent
Cas No.:4213-45-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Quinacrine mustard is a fluorescent DNA-intercalating agent.1 It selectively binds to adenine-thymine (AT) base pairs over guanine-cytosine (GC) base pairs.2,3 Quinacrine mustard has been used to label metaphase chromosomes for karyotyping by autoradiography.1 It displays excitation/emission maxima of 425/480 nm, respectively.4
|1. Caspersson, T., Farber, S., Foley, G.E., et al. Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49(1), 219-222 (1968).|2. Ellison, J.R., and Barr, H.J. Quinacrine fluorescence of specific chromosome regions. Late replication and high A:T content in Samoaia leonensis. Chromosoma 36(4), 375-390 (1972).|3. Weisblum, B., and De Haseth, P.L. Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylate-rich regions in DNA. Proc. Natl. Acad. Sci. U.S.A. 69(3), 629-632 (1972).|4. Chen, R.F. Fluorescence of quinacrine mustard conjugated to proteins. Arch. Biochem. Biophys. 172(1), 39-50 (1976).
| Cas No. | 4213-45-0 | SDF | |
| 别名 | 氮芥喹丫因 | ||
| Canonical SMILES | COC1=CC2=C(C=C1)N=C3C(C=CC(Cl)=C3)=C2NC(CCCN(CCCl)CCCl)C.Cl.Cl | ||
| 分子式 | C23H28Cl3N3O•2HCl | 分子量 | 541.8 |
| 溶解度 | Chloroform: soluble,Water: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8457 mL | 9.2285 mL | 18.457 mL |
| 5 mM | 0.3691 mL | 1.8457 mL | 3.6914 mL |
| 10 mM | 0.1846 mL | 0.9228 mL | 1.8457 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
The radiologic appearance of chemical pleurodesis
Radiology 1980 May;135(2):313-7.PMID:7367618DOI:10.1148/radiology.135.2.7367618.
Intrapleural instillation of drugs (chemical pleurodesis) was performed in 57 patients with malignant pleural effusions or recurrent pneumothorax. Agents used included mechlorethamine hydrochloride (nitrogen mustard), quinacrine hydrochloride (Atabrine), tetracycline, and others. The most frequent finding on chest radiographs (56%) was multiple loculated air-fluid levels, an appearance simulating an empyema. Resolution of these changes occurred in one to three weeks. Late sequelae included pleural thickening (63%) or the development of a severe fibrothorax. In the majority of the six patients treated for recurrent pneumothoraces, the radiographic appearance returned to normal. The appearance of a new air-fluid level following withdrawal of the chest tube in one patient indicated a complicating empyema.
Delayed response of QM- and DA/DAPI-fluorescence in C-heterochromatin of the small Japanese field mouse, Apodemus argenteus
Zoolog Sci 1997 Feb;14(1):57-64.PMID:9200979DOI:10.2108/zsj.14.57.
The small Japanese field mouse Apodemus argenteus has the diploid chromosome number of 46, carrying rather large centromeric C-heterochromatin in most of the 44 autosomes and a large amount of C-heterochromatin in the sex chromosomes: the largest subtelocentric X was heterochromatic in almost two-fifth (whole short arm and proximal part of the long arm) of its entire length and the medium-sized acrocentric Y was totally heterochromatic. The C-heterochromatin (C-positive) areas, other than those of the Y and smallest three pairs, had a unique property of "delayed QM-fluorescence", which has not been reported to-date, showing dull QM-fluorescence immediately after exposure to blue light (BL), but gradually turning to bright fluorescence in a few minutes. The fluorescence intensity gradually decreased after attaining its peak, and finally became extinct. A similar pattern of fluorescence was also obtained in DA/DAPI-stained-X chromosome C-heterochromatin, but not in autosomal C-heterochromatin. No such dull-to-bright transition of QM-fluorescence could be obtained by CMA staining, for which the C-positive areas were apparently negative even after overexposure to BL. These facts indicate that the C-positive areas of A. argenteus showing dull-to-bright transition of QM-fluorescence contain A-T rich DNA. The delayed QM-fluorescence was found only in A. argenteus, in thirteen mammalian species so-far examined. Furthermore, this unique property of QM-fluorescence could be artificially altered to non-delayed ordinary type of fluorescence by sequentially pretreating the fixed chromosomes with hydrochloride and barium hydroxide solutions. The cytological implication of the delayed fluorescence in the C-heterochromatin of A. argenteus is briefly discussed.