(R)-Bromoenol lactone-d7

(R)-Bromoenol lactone-d₇ ((R)-BEL-d₇) contains seven deuterium atoms at the 2, 3, 4, 5, 6, 7, and 8 positions. It is intended for use as an internal standard for the quantification of (R)-BEL by GC- or LC-mass spectrometry (MS). The phospholipases are an extensive family of lipid hydrolases that function in cell signaling, digestion, membrane remodeling, and as venom components.¹ The calcium-independent phospholipase A₂ (iPLA₂) are a PLA₂ subfamily closely associated with the release of arachidonic acid in response to physiologic stimuli. (R)-Bromoenol lactone ((R)-BEL) is an irreversible, chiral, mechanism-based inhibitor of iPLA₂γ. Unlike (S)-BEL, (R)-BEL does not inhibit iPLA₂β except at high doses of 20-30 µM.² (R)-BEL inhibits human recombinant iPLA₂γ with an IC₅₀ of approximately 0.6 µM.

1.Balsinde, J., Balboa, M.A., Insel, P.A., et al.Regulation and inhibition of phospholipase A2Annual Reviews of Pharmacology and Toxicology39175-189(1999) 2.Jenkins, C.M., Han, X., Mancuso, D.J., et al.Identification of calcium-independent phospholipase A2 (iPLA2)β, and not iPLA2γ, as the mediator of arginine vasopressin-induced arachidonic acid release in A-10 smooth muscle cellsThe Journal of Biological Chemisty277(36)32807-32814(2002)