Resazurin sodium salt
(Synonyms: 刃天青钠; Diazoresorcinol sodium) 目录号 : GC16113
A dye used as an oxidation-reduction indicator
Cas No.:62758-13-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >94.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
F. tularensis is cultured in TSBc supplemented with 44 μM Resazurin sodium salt at 37°C with shaking for 24 h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 nm and 570 nm to detect the presence of Resazurin sodium salt and resorufin, respectively. The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time[1]. |
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References: [1]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93. |
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The non-fluorescent resazurin has been used as a fluorogenic oxidation-reduction indicator in various cells by flow cytometry, fluorescence microscopy and high-throughput screening. Its red-fluorescent product, resorufin, has absorption/emission maxima ~575/585 nm.
In vitro: Resazurin has been applied as a simple and versatile way to measure cell proliferation and cytotoxicity. Although resazurin presented plenty of advantages over other cytotoxicity or proliferation tests, everal drawbacks to the routine use of resazurin were also observed. Previoius studies showed accumulation of the fluorescent product of resazurin in the medium which could result in an overestimation of cell population. Moreover, the extensive reduction of resazurin by metabolically active cells would lead to a final nonfluorescent product, and thus an underestimation of cellular activity [1]. In order to evaluate the effect of resazurin over longer incubation times, MCF7, MCF10A, 3T3-L1 and D1 cell lines were tested. Results showed that MCF7, 3T3-L1, and D1 cells cultured for 8 days with 20% resazurin had significantly less cell survivability. Moreover, levels of metabolic activity, DNA concentration, as well as glucose consumption of the cell lines in medium with 20% resazurin were significantly lower. Data also showed that the toxicities were more pronounced in the cancer cell lines than in the noncancerous cells [2].
References:
[1] O'Brien J,Wilson I,Orton T,Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem.2000 Sep;267(17):5421-6.
[2] Pace RT,Burg KJ. Toxic effects of resazurin on cell cultures. Cytotechnology.2015 Jan;67(1):13-7.
| Cas No. | 62758-13-8 | SDF | |
| 别名 | 刃天青钠; Diazoresorcinol sodium | ||
| 化学名 | sodium 7-oxido-3-oxo-3H-phenoxazine 10-oxide | ||
| Canonical SMILES | O=C1C=C2OC3=CC([O-])=CC=C3[N+]([O-])=C2C=C1.[Na+] | ||
| 分子式 | C12H6NNaO4 | 分子量 | 251.17 |
| 溶解度 | ≥ 25.1mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9814 mL | 19.9068 mL | 39.8137 mL |
| 5 mM | 0.7963 mL | 3.9814 mL | 7.9627 mL |
| 10 mM | 0.3981 mL | 1.9907 mL | 3.9814 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。