Maslinic Acid
(Synonyms: 山楂酸; Crategolic acid; 2α-Hydroxyoleanolic acid) 目录号 : GC17632
Maslinic Acid是一种五环三萜酸，广泛存在于橄榄和其他植物中。Maslinic Acid具有抗炎、抗氧化、抗肿瘤、降血糖和神经保护等多种生物活性。
Cas No.:4373-41-5
Sample solution is provided at 25 µL, 10mM.
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Maslinic acid is a pentacyclic triterpene acid widely found in olives and other plants. Maslinic acid has multiple biological activities such as anti-inflammatory, antioxidant, anti-tumor, hypoglycemic, and neuroprotective[1].
Maslinic Acid (1-10μM; 24h) was proved to induce cell autophagy by disrupting the combination of Bcl2 and Beclin1 in rat PC12 cells[2]. Maslinic acid (6.25, 12.5, 25, 50mM; 48h) significantly decreased lipid accumulation in oleic acid-incubated HepG2 cells[3]. Maslinic acid (0, 25, 30, 35, 40, 45μM; 48h) decreased total cell viability and induced DNA condensation, damage, and fragmentation in HeLa cells[4].
Maslinic Acid (5, 10, 20mg/kg; ip; 8 weeks) treatment for 8 weeks repressed the oxidative stress and inflammation in the kidney of diabetic mice[5]. Maslinic acid (0.3, 1, 3mg/kg; po; administered 3, 6, 9, 12h before kill) enhanced the ERK–CREB and PI3K–Akt signalling pathways in the hippocampus[6].
References:
[1]He Y, Wang Y, Yang K, et al. Maslinic acid: a new compound for the treatment of multiple organ diseases[J]. Molecules, 2022, 27(24): 8732.
[2]Germano A, Saba L, De Francia S, et al. CYP11B1 has no role in mitotane action and metabolism in adrenocortical carcinoma cells[J]. PloS one, 2018, 13(5): e0196931.
[3]Liou C J, Dai Y W, Wang C L, et al. Maslinic acid protects against obesity-induced nonalcoholic fatty liver disease in mice through regulation of the Sirt1/AMPK signaling pathway[J]. The FASEB Journal, 2019, 33(11): 11791.
[4]Lu K W, Yang M D, Peng S F, et al. Maslinic acid induces DNA damage and impairs DNA repair in human cervical cancer HeLa cells[J]. Anticancer Research, 2020, 40(12): 6869-6877.
[5]Gao H, Wu H. Maslinic acid activates renal AMPK/SIRT1 signaling pathway and protects against diabetic nephropathy in mice[J]. BMC Endocrine Disorders, 2022, 22(1): 25.
[6]Bae H J, Kim J, Kim J, et al. The effect of maslinic acid on cognitive dysfunction induced by cholinergic blockade in mice[J]. British Journal of Pharmacology, 2020, 177(14): 3197-3209.
Maslinic Acid是一种五环三萜酸，广泛存在于橄榄和其他植物中。Maslinic Acid具有抗炎、抗氧化、抗肿瘤、降血糖和神经保护等多种生物活性[1]。
Maslinic Acid（1-10μM；24h）可通过破坏大鼠PC12细胞中Bcl2和Beclin1的结合诱导细胞自噬[2]。Maslinic Acid（6.25、12.5、25、50mM；48h）可显著减少油酸诱导的HepG2细胞的脂质积累[3]。Maslinic Acid（0、25、30、35、40、45μM；48h）会降低HeLa细胞的总存活率，并诱导DNA凝聚、损伤和破碎[4]。
Maslinic Acid（5、10、20mg/kg；ip；8weeks）治疗 8 周可抑制糖尿病小鼠肾脏的氧化应激和炎症[5]。Maslinic Acid（0.3、1、3mg/kg；po；在处死前3、6、9和12h给药）增强了海马中 ERK-CREB 和 PI3K-Akt 信号通路[6]。
| Cell experiment [1]: | |
Cell lines | PC12 cells |
Preparation Method | Before drug treatment, cells were subcultured in 6-well plates and the medium was changed to DMEM containing 2% FBS. The day after cell adhesion, different concentrations of Maslinic Acid and positive control rapamycin (Rap) (10μM) were added to different wells and incubated for 24 hours. |
Reaction Conditions | 1-10μM; 24h |
Applications | Maslinic Acid was proved to induce cell autophagy by disrupting the combination of Bcl2 and Beclin1 in rat PC12 cells. |
| Animal experiment [2]: | |
Animal models | Diabetic mouse model |
Preparation Method | Streptozocin was used to induce diabetic mouse model. Fasting blood glucose levels > 300mg/dL was regarded as the incidence of diabetes. Diabetic mice of similar body weight were randomly divided into four groups: STZ (diabetic control group), MA5 (diabetic mice treated with 5mg/kg Maslinic Acid), MA10 (diabetic mice treated with 10mg/kg Maslinic Acid), and MA20 (diabetic mice treated with 20mg/kg Maslinic Acid). Normal mice were in the normal control group (NC). Maslinic acid was injected intraperitoneally every 3 days after diabetes induction for 8 weeks. The changes in body weight and fasting glucose levels of mice were evaluated, respectively. |
Dosage form | 5, 10, 20mg/kg; q3d; ip; 8 weeks |
Applications | Maslinic Acid treatment for 8 weeks repressed the oxidative stress and inflammation in the kidney of diabetic mice. |
References: | |
| Cas No. | 4373-41-5 | SDF | |
| 别名 | 山楂酸; Crategolic acid; 2α-Hydroxyoleanolic acid | ||
| 化学名 | 2α,3β-dihydroxy-olean-12-en-28-oic acid | ||
| Canonical SMILES | CC1(C)CC[C@]2(C(O)=O)CC[C@@]3(C)[C@]4(C)CCC5C(C)(C)[C@@H](O)[C@H](O)C[C@]5(C)[C@@]4([H])CC=C3[C@@]2([H])C1 | ||
| 分子式 | C30H48O4 | 分子量 | 472.7 |
| 溶解度 | ≤0.5mg/ml in ethanol;20mg/ml in DMSO;15mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1155 mL | 10.5775 mL | 21.1551 mL |
| 5 mM | 0.4231 mL | 2.1155 mL | 4.231 mL |
| 10 mM | 0.2116 mL | 1.0578 mL | 2.1155 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet