Abietic Acid
(Synonyms: 松香酸) 目录号 : GC40831
A diterpene with diverse biological properties
Cas No.:514-10-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Abietic acid is an abietane diterpene originally isolated from wood resin that has diverse biological activities, including enzyme inhibitory, antiproliferative, antibacterial, and anti-obesity properties. It inhibits soybean 5-lipoxygenase (5-LO) and rat prostate testosterone 5α-reductase in cell-free assays (IC50s = 29.5 and 56 μM, respectively). Abietic acid selectively inhibits proliferation of HeLa cervical cancer cells over noncancerous Vero cells (IC50s = 15 and 53 μM, respectively) and inhibits the growth of S. epidermidis, P. acnes, and S. mitis bacteria (MICs = 8, 4, and 16 μg/ml, respectively). It induces expression of fatty acid binding protein 2 and lipoprotein lipase (LPL) in differentiated 3T3-L1 mouse adipocytes when used at a concentration of 25 μM. Abietic acid (40 mg/kg per day) also decreases body and adipose tissue weight in mice fed a high-fat diet.
| Cas No. | 514-10-3 | SDF | |
| 别名 | 松香酸 | ||
| Canonical SMILES | CC(C)C1=CC2=CC[C@@]3([H])[C@](C)(C(O)=O)CCC[C@]3(C)[C@@]2([H])CC1 | ||
| 分子式 | C20H30O2 | 分子量 | 302.5 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:20): 0.04 mg/ml,Ethanol: 20 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3058 mL | 16.5289 mL | 33.0579 mL |
| 5 mM | 0.6612 mL | 3.3058 mL | 6.6116 mL |
| 10 mM | 0.3306 mL | 1.6529 mL | 3.3058 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Abietic Acid ameliorates psoriasis-like inflammation and modulates gut microbiota in mice
J Ethnopharmacol 2021 May 23;272:113934.PMID:33607198DOI:10.1016/j.jep.2021.113934.
Ethnopharmacological relevance: Abietic Acid (AA), an antibacterial terpenoid, was initially isolated from rosin which has been used as a traditional Chinese medicine to treat psoriasis. In our previous works, we found that water-processed rosin (WPR) can alleviate imiquimod (IMQ)-induced psoriasis-like inflammation in mice. However, the efficacy of AA, the main component of WPR, against psoriasis remains unclear. Materials and methods: In this study, we confirmed the anti-psoriasis efficacy of AA (40 mg/kg daily for 7 days) in IMQ-induced psoriasis-like inflammation BALB/c mouse model by the psoriasis area severity index (PASI), flow cytometry, ELISA, histopathological and immunohistochemical analysis. Furthermore, we detected the relative abundance of gut microbe using high-throughput 16S rRNA gene sequencing to validate whether AA modulate gut microbe. Result: Oral administration of AA ameliorates IMQ-induced psoriasis-like skin inflammation through reducing PASI scores, regulating the balance of Th17/Treg cells in the mouse spleen, and downregulating the level of serum cytokines such as TNF-α, IL-17A, TGF-1β, and IL-23. 16S rRNA gene sequencing revealed that the relative abundance of gut bacteria related to inflammation, such as, Anaerotruncus and Christensenella at genus level were decreased, while Kurthia, Citrobacter, and Klebsiella at genus level were increased in AA group mice. Additionally, the correlation analysis illustrated that the key microbiota had a close relationship with the psoriasis-like inflammation related indexes. Conclusion: AA might exert the anti-psoriasis effect via inhibiting Th17-related immune responses, hinting that it might be a candidate for treating psoriasis. Meanwhile, the alteration of intestinal microbiota by AA treatment is another possible explanation for the amelioration of imiquimod-induced psoriasis-like inflammation.
Abietic Acid attenuates IL-1β-induced inflammation in human osteoarthritis chondrocytes
Int Immunopharmacol 2018 Nov;64:110-115.PMID:30172103DOI:10.1016/j.intimp.2018.07.014.
Abietic Acid has been reported to have anti-inflammatory activity. However, whether Abietic Acid has anti-inflammatory effects against osteoarthritis (OA) remains unclear. The present study aimed to measure the anti-inflammatory effects of Abietic Acid on OA in vitro. Human osteoarthritis chondrocytes were pretreated with Abietic Acid 1 h before IL-1β treatment. The results showed that treatment of Abietic Acid significantly inhibited IL-1β-induced TNF-α, NO, PGE2 production, and COX-2 expression. Abietic Acid also concentration-dependently suppressed MMP1, MMP3, and MMP13 production induced by IL-1β. Moreover, the increased phosphorylation levels of NF-κB p65 and IκBα were inhibited by the treatment of Abietic Acid. We also found that the expression of PPAR-γ was increased by Abietic Acid. The inhibition of Abietic Acid on TNF-α, NO, and PGE2 production were reversed by GW9662, the inhibitor of PPAR-γ. In conclusion, the study elucidated Abietic Acid suppressed IL-1β-induced inflammation in human osteoarthritis chondrocytes by activating PPAR-γ.
Abietic Acid inhibits acetaminophen-induced liver injury by alleviating inflammation and ferroptosis through regulating Nrf2/HO-1 axis
Int Immunopharmacol 2023 May;118:110029.PMID:36963265DOI:10.1016/j.intimp.2023.110029.
Abietic Acid has been known to exhibit anti-inflammatory activity. This study was designed to investigate the protective effects of Abietic Acid on acetaminophen (APAP)-induced liver injury. The data demonstrated that Abietic Acid significantly ameliorated APAP-induced liver pathological changes, TNF-α and IL-1β production. APAP could increase malondialdehyde (MDA) and Fe2+ levels, and decrease ATP and glutathione (GSH) levels, as well as glutathione peroxidase 4 (GPX4) and xCT expression. However, these changes induced by APAP were prevented by Abietic Acid, indicating Abietic Acid could inhibit APAP-induced ferroptosis. Furthermore, Abietic Acid inhibited APAP-induced NF-κB activation and increased the expression of Nrf2 and HO-1. Additionally, the inhibitory effects of Abietic Acid on APAP-induced liver injury were prevented in Nrf2-/- mice. In vitro, the inhibition of Abietic Acid on APAP-induced inflammation and ferroptosis were reversed when Nrf2 was knockdown. In summary, abietic acidexhibited a therapeutic effectagainst liver injury by attenuating inflammation and ferroptosis.
Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius
Antibiotics (Basel) 2021 Jan 15;10(1):80.PMID:33467635DOI:10.3390/antibiotics10010080.
Resin acids are valued in traditional medicine for their antiseptic properties. Among these, Abietic Acid has been reported to be active against methicillin-resistant Staphylococcus aureus (MRSA) strains. In veterinary healthcare, the methicillin-resistant Staphylococcus pseudintermedius (MRSP) strain is an important reservoir of antibiotic resistance genes including mecA. The incidence of MRSP has been increasing, and treatment options in veterinary medicine are partial. Here, we investigated the antimicrobial and antibiofilm properties of Abietic Acid against three MRSP and two methicillin-susceptible Staphylococcus pseudintermedius (MSSP) strains, isolated from diseased pet animals and human wound samples. Abietic Acid showed a significant minimal inhibitory concentration (MIC) value ranging from 32 to 64 μg/mL (MRSPs) and 8 μg/mL (MSSP). By checkerboard method we demonstrated that Abietic Acid increased oxacillin susceptibility of MRSP strains, thus showing a synergistic interaction with oxacillin. Abietic Acid was also able to contrast the vitality of treated MSSP and MRSP1 biofilms at 20 μg/mL and 40 μg/mL, respectively. Finally, the compound moderately reduced mecA, mecR1 and mec1 gene expression. In conclusion, the results here reported demonstrate the antimicrobial activity of Abietic Acid against MRSP and support the use of this compound as a potential therapeutic agent to be used in combinatorial antibiotic therapy.
Abietic Acid alleviates endoplasmic reticulum stress and lipid accumulation in human primary hepatocytes through the AMPK/ORP150 signaling
Biochem Biophys Res Commun 2022 Jun 11;608:142-148.PMID:35398611DOI:10.1016/j.bbrc.2022.04.010.
Abietic Acid (AA), the main component of pine resin that has been traditionally used as Asian medicine, has been reported to demonstrate anti-inflammatory activities. Despite this, little is known about the effects of AA on hepatic endoplasmic reticulum (ER) stress and lipid metabolism. This study investigated the impacts of AA on ER stress and steatosis in in vitro obesity models. We found that Treatment with AA reduced lipid deposition and lipogenesis-related proteins expression in human primary hepatocytes. Augmented expression of ER stress markers (phospho-eukaryotic initiation factor-2α (eIF2α) and C/EBP homologous protein (CHOP)) in palmitate-treated hepatocytes were reversed by AA treatment. Further, AA treatment increased the expression of phospho-AMPK and oxygen-regulated protein 150 (ORP150) in hepatocytes. siRNA-associated knockdown of AMPK or ORP150 expression reduced the effects of AA on not only hepatic ER stress but also lipogenesis and apoptosis. These results denote that AA attenuates lipid accumulation in hepatocytes in the presence of palmitate through the suppression of ER stress by AMPK/ORP150 signaling. AA could be a potential candidate for treating non-alcoholic fatty liver disease.