Diazolidinyl urea
(Synonyms: 重氮烷基脲) 目录号 : GC60766
Diazolidinyl urea (Germall II) is an antimicrobial preservative used in cosmetics.
Cas No.:78491-02-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Diazolidinyl urea (Germall II) is an antimicrobial preservative used in cosmetics.
| Cas No. | 78491-02-8 | SDF | |
| 别名 | 重氮烷基脲 | ||
| Canonical SMILES | O=C(NCO)N(C(C(N1CO)=O)N(CO)C1=O)CO | ||
| 分子式 | C8H14N4O7 | 分子量 | 278.22 |
| 溶解度 | DMSO: 250 mg/mL (898.57 mM); Water: 166.67 mg/mL (599.06 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.5943 mL | 17.9714 mL | 35.9428 mL |
| 5 mM | 0.7189 mL | 3.5943 mL | 7.1886 mL |
| 10 mM | 0.3594 mL | 1.7971 mL | 3.5943 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Contact Dermatitis to Medications and Skin Products
Clin Rev Allergy Immunol 2019 Feb;56(1):41-59.PMID:30145645DOI:10.1007/s12016-018-8705-0.
Consumer products and topical medications today contain many allergens that can cause a reaction on the skin known as allergic contact dermatitis. This review looks at various allergens in these products and reports current allergic contact dermatitis incidence and trends in North America, Europe, and Asia. First, medication contact allergy to corticosteroids will be discussed along with its five structural classes (A, B, C, D1, D2) and their steroid test compounds (tixocortol-21-pivalate, triamcinolone acetonide, budesonide, clobetasol-17-propionate, hydrocortisone-17-butyrate). Cross-reactivities between the steroid classes will also be examined. Next, estrogen and testosterone transdermal therapeutic systems, local anesthetic (benzocaine, lidocaine, pramoxine, dyclonine) antihistamines (piperazine, ethanolamine, propylamine, phenothiazine, piperidine, and pyrrolidine), topical antibiotics (neomycin, spectinomycin, bacitracin, mupirocin), and sunscreen are evaluated for their potential to cause contact dermatitis and cross-reactivities. Finally, we examine the ingredients in the excipients of these products, such as the formaldehyde releasers (quaternium-15, 2-bromo-2-nitropropane-1,3 diol, Diazolidinyl urea, imidazolidinyl urea, DMDM hydantoin), the non-formaldehyde releasers (isothiazolinones, parabens, methyldibromo glutaronitrile, iodopropynyl butylcarbamate, and thimerosal), fragrance mixes, and Myroxylon pereirae (Balsam of Peru) for contact allergy incidence and prevalence. Furthermore, strategies, recommendations, and two online tools (SkinSAFE and the Contact Allergen Management Program) on how to avoid these allergens in commercial skin care products will be discussed at the end.
The different decomposition properties of Diazolidinyl urea in cosmetics and patch test materials
Contact Dermatitis 2011 Aug;65(2):81-91.PMID:21309789DOI:10.1111/j.1600-0536.2010.01862.x.
Background: Diazolidinyl urea is a formaldehyde-releasing compound that releases formaldehyde through its decomposition. However, there have been few reports about the decomposition properties of Diazolidinyl urea in cosmetics and patch test materials. Objectives: The aim of this study was to show how Diazolidinyl urea decomposes in cosmetics and patch test vehicles, and to determine which cosmetic compounds should be evaluated in patch test studies of Diazolidinyl urea. Method: We fractionated diazolidinyl urea-dissolving buffers or diazolidinyl urea-containing cosmetics with high-performance liquid chromatography-photodiode array detector (HPLC-PDA), and characterized them in liquid chromatography-mass spectrometry (LC-MS) and (1) H-nuclear magnetic resonance studies. Diazolidinyl urea-containing cosmetics and Diazolidinyl urea patch test materials were also analysed with HPLC-PDA and LC-MS. Results: Diazolidinyl urea was decomposed to (4-hydroxymethyl-2,5-dioxo-imidazolidine-4-yl)-urea (HU) and (3,4-bis-hydroxymethyl-2,5-dioxo-imidazolidine-4-yl)-urea (3,4-BHU) in most of the cosmetic samples tested. The peak patterns of the patch test materials analysed with the HPLC-PDA were different from those of the cosmetic samples. CONCLUSIONS. The diazolidinyl urea-derived decomposition products differed between the cosmetics and patch test preparations. To test the contact sensitivity of the Diazolidinyl urea present in cosmetics, patch tests with HU and 3,4-BHU in petrolatum should be performed.
Simultaneous measurement of Diazolidinyl urea, urea, and allantoin in cosmetic samples by hydrophilic interaction chromatography
J Chromatogr B Analyt Technol Biomed Life Sci 2009 Apr 1;877(10):1005-10.PMID:19269905DOI:10.1016/j.jchromb.2009.02.032.
A new HPLC method for simultaneous measurement of Diazolidinyl urea (DU), urea, and allantoin by hydrophilic interaction chromatography using a column packed with triazol-bonded silica particles is described. The calibration curves of DU, urea, and allantoin were linear over the ranges 2.5-125.0, 30-1250, and 0.25-18.75mg/L, respectively. The recoveries of DU, urea, and allantoin from homemade cosmetic samples ranged from 92.84% to 101.69%, 98.19% to 103.22%, and 95.75% to 102.09%, respectively. The peak relative standard deviation (RSD) values for the recovery tests of 3 concentrations/5 replicates were 3.03% for all compounds. In day-to-day measurement of recovery tests from homemade lotions over 3 consecutive days, the RSDs were less than 2.5% in all cases. This method was well validated and would be helpful for quickly analyzing these compounds in cosmetic samples.
Characterization and chemistry of imidazolidinyl urea and Diazolidinyl urea
Contact Dermatitis 2006 Jan;54(1):50-8.PMID:16426294DOI:10.1111/j.0105-1873.2006.00735.x.
For several decades, the cosmetic preservatives imidazolidinyl urea (IU) and Diazolidinyl urea (DU) have not only been poorly characterized but have also had misleading chemical structures assigned to them. The most common trade names of IU and DU are Germall 115 and Germall II, respectively. This publication gives an insight into what these 2 well-known contact allergens consist of and their degradation patterns. Approximately, 30-40% of both products can be characterized by mixtures of allantoin (synthetic starting material), (4-hydroxymethyl-2,5-dioxo-imidazolidin-4-yl)-urea (compound HU) and presumably 1-(3,4-bis-hydroxymethyl-2,5-dioxo-imidazolidin-4-yl)-1,3-bis-hydroxymethyl-urea (compound BHU). A full chemical characterization of compound HU is shown. The remaining part of both IU and DU are believed to be polymers of allantoin-formaldehyde condensation products. The analytical methods used to characterize IU and DU are capillary electrophoresis and nuclear magnetic resonance and mass spectroscopy studies.
Experimental delayed contact sensitization to Diazolidinyl urea (Germall II) in guinea pigs
Contact Dermatitis 1987 Mar;16(3):164-8.PMID:3581825DOI:10.1111/j.1600-0536.1987.tb01413.x.
Diazolidinyl urea (Germall II) is a new preservative recommended for use in certain consumer products. Although 2 reports document the human sensitization rates of this preservative, no publications quantify its sensitization potential in controlled animal experiments. Diazolidinyl urea induced mild sensitization (grade 2) under maximization test conditions. Further, there was evidence of cross-reactions with both imidazolidinyl urea (Germall 115) and formaldehyde in diazolidinyl-urea-sensitized animals. Rechallenge of diazolidinyl-urea-sensitized animals with Diazolidinyl urea 4 weeks following the primary challenge only elicited a weak response (0.5) from 1 animal out of 8.