Buparvaquone
(Synonyms: 布帕伐醌) 目录号 : GC35569
An electron transport chain inhibitor
Cas No.:88426-33-9
Sample solution is provided at 25 µL, 10mM.
Buparvaquone is a hydroxynaphthoquinone that inhibits electron transport by blocking cytochrome bc1 in parasites that cause leishmaniasis.1 Formulations containing buparvaquone are used to treat theileriosis, an infection by the parasites T. annulata and T. parva (in vitro EC50s of 1.5 x 10-8 M and 6.1 x 10-10 M, respectively).2,3
1.Ortiz, D., Forquer, I., Boitz, J., et al.Targeting the cytochrome bc1 complex of Leishmania parasites for discovery of novel drugsAntimicrob. Agents and Chemother.60(8)4972-4982(2016) 2.Abdou, T.A., Abou-El-naga, T.R., and Mahmoud, M.A.Clinicopathological studies on Theileria annulata infection in Siwa oasis in Egypt56th Annual Meeting of the European Association for Animal Production70(2005) 3.Hudson, A.T., Randall, A.W., Fry, M., et al.Novel anti-malarial hydroxynaphthoquinones with potent broad spectrum anti-protozoal activityParasitology90(Pt 1)45-55(1985)
Cell experiment: | To study whether pretreatment of host cells prior to invasion had any effect on parasite proliferation, confluent HFF grown in 6-well plates are treated with 1 µM buparvaquone in medium for 1 h or 5 h, and controls are exposed to the corresponding amounts of DMSO. Subsequently, the drug-containing medium is removed and monolayers are ished 4 times with Hank's Balanced Salt Solution, and are infected with Nc-Liv tachyzoites in 5 mL medium without any drug or solvent. After 2 days, cells are collected with a cell scraper, centrifuged, ished once more in PBS, and the pellet is stored at −20 °C prior to quantification of N. caninum proliferation by N. caninum-specific real time PCR as outlined below[1]. |
Animal experiment: | Mice: On day 0, all mice are infected by intraperitoneal (i.p.) injection of freshly purified N. caninum tachyzoites. After 48 h, mice receive BPQ (100 mg/kg) as suspension in corn oil either by i.p. injection of a volume of 100 µl or by oral application of 100 µl by gavage. The control groups obtained the corresponding amount of the solvent only, either i.p. or orally (see Table 2). The treatments are performed 5 times on a daily basis. If not indicated otherwise, mice are inspected twice daily for clinical signs (ruffled coat, apathy, hind limb paralysis) until day 21 post infection (p.i.), at which time they are euthanized[1]. |
References: [1]. Müller J, et al. Buparvaquone is active against Neospora caninum in vitro and in experimentally infected mice. Int J Parasitol Drugs Drug Resist. 2015 Feb 13;5(1):16-25. |
Cas No. | 88426-33-9 | SDF | |
别名 | 布帕伐醌 | ||
Canonical SMILES | O=C1C(CC2CCC(C(C)(C)C)CC2)=C(O)C(C3=C1C=CC=C3)=O | ||
分子式 | C21H26O3 | 分子量 | 326.43 |
溶解度 | DMSO: 33.33 mg/mL (102.10 mM); DMF: 25 mg/mL (76.59 mM); Ethanol: 2 mg/mL (6.13 mM); Water: < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 3.0634 mL | 15.3172 mL | 30.6344 mL |
5 mM | 0.6127 mL | 3.0634 mL | 6.1269 mL |
10 mM | 0.3063 mL | 1.5317 mL | 3.0634 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet