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Buparvaquone Sale

(Synonyms: 布帕伐醌) 目录号 : GC35569

An electron transport chain inhibitor

Buparvaquone Chemical Structure

Cas No.:88426-33-9

规格 价格 库存 购买数量
10mM (in 1mL DMF)
¥146.00
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5mg
¥133.00
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10mg
¥188.00
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25mg
¥308.00
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50mg
¥419.00
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100mg
¥553.00
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Sample solution is provided at 25 µL, 10mM.

Description

Buparvaquone is a hydroxynaphthoquinone that inhibits electron transport by blocking cytochrome bc1 in parasites that cause leishmaniasis.1 Formulations containing buparvaquone are used to treat theileriosis, an infection by the parasites T. annulata and T. parva (in vitro EC50s of 1.5 x 10-8 M and 6.1 x 10-10 M, respectively).2,3

1.Ortiz, D., Forquer, I., Boitz, J., et al.Targeting the cytochrome bc1 complex of Leishmania parasites for discovery of novel drugsAntimicrob. Agents and Chemother.60(8)4972-4982(2016) 2.Abdou, T.A., Abou-El-naga, T.R., and Mahmoud, M.A.Clinicopathological studies on Theileria annulata infection in Siwa oasis in Egypt56th Annual Meeting of the European Association for Animal Production70(2005) 3.Hudson, A.T., Randall, A.W., Fry, M., et al.Novel anti-malarial hydroxynaphthoquinones with potent broad spectrum anti-protozoal activityParasitology90(Pt 1)45-55(1985)

实验参考方法

Cell experiment:

To study whether pretreatment of host cells prior to invasion had any effect on parasite proliferation, confluent HFF grown in 6-well plates are treated with 1 µM buparvaquone in medium for 1 h or 5 h, and controls are exposed to the corresponding amounts of DMSO. Subsequently, the drug-containing medium is removed and monolayers are ished 4 times with Hank's Balanced Salt Solution, and are infected with Nc-Liv tachyzoites in 5 mL medium without any drug or solvent. After 2 days, cells are collected with a cell scraper, centrifuged, ished once more in PBS, and the pellet is stored at −20 °C prior to quantification of N. caninum proliferation by N. caninum-specific real time PCR as outlined below[1].

Animal experiment:

Mice: On day 0, all mice are infected by intraperitoneal (i.p.) injection of freshly purified N. caninum tachyzoites. After 48 h, mice receive BPQ (100 mg/kg) as suspension in corn oil either by i.p. injection of a volume of 100 µl or by oral application of 100 µl by gavage. The control groups obtained the corresponding amount of the solvent only, either i.p. or orally (see Table 2). The treatments are performed 5 times on a daily basis. If not indicated otherwise, mice are inspected twice daily for clinical signs (ruffled coat, apathy, hind limb paralysis) until day 21 post infection (p.i.), at which time they are euthanized[1].

References:

[1]. Müller J, et al. Buparvaquone is active against Neospora caninum in vitro and in experimentally infected mice. Int J Parasitol Drugs Drug Resist. 2015 Feb 13;5(1):16-25.
[2]. Reim o JQ, et al. Effectiveness of liposomal buparvaquone in an experimental hamster model of Leishmania (L.) infantum chagasi. Exp Parasitol. 2012 Mar;130(3):195-9.
[3]. Garnier T, et al. In vivo studies on the antileishmanial activity of buparvaquone and its prodrugs. J Antimicrob Chemother. 2007 Oct;60(4):802-10.

化学性质

Cas No. 88426-33-9 SDF
别名 布帕伐醌
Canonical SMILES O=C1C(CC2CCC(C(C)(C)C)CC2)=C(O)C(C3=C1C=CC=C3)=O
分子式 C21H26O3 分子量 326.43
溶解度 DMSO: 33.33 mg/mL (102.10 mM); DMF: 25 mg/mL (76.59 mM); Ethanol: 2 mg/mL (6.13 mM); Water: < 0.1 mg/mL (insoluble) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 3.0634 mL 15.3172 mL 30.6344 mL
5 mM 0.6127 mL 3.0634 mL 6.1269 mL
10 mM 0.3063 mL 1.5317 mL 3.0634 mL
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