Metaflumizone
(Synonyms: 氰氟虫腙; BAS-320I) 目录号 : GC34665
Metaflumizone是一种缩氨基脲杀虫剂，为有效的钠离子通道(sodiumchannel)阻滞剂。
Cas No.:139968-49-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Metaflumizone is a semicarbazone insecticide, acts as a potent sodium channel blocker[1]. Sodium channel[1]
Metaflumizone (10 μM) completely inhibits the Para/TipE sodium currents in Xenopus oocytes[1].
[1]. Salgado VL, et al. Metaflumizone is a novel sodium channel blocker insecticide. Vet Parasitol. 2007 Dec 15;150(3):182-9. Epub 2007 Oct 23.
| Cas No. | 139968-49-3 | SDF | |
| 别名 | 氰氟虫腙; BAS-320I | ||
| Canonical SMILES | FC(F)(F)C1=CC(/C(CC2=CC=C(C=C2)C#N)=N\NC(NC3=CC=C(C=C3)OC(F)(F)F)=O)=CC=C1 | ||
| 分子式 | C24H16F6N4O2 | 分子量 | 506.4 |
| 溶解度 | DMSO : 300 mg/mL (592.42 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9747 mL | 9.8736 mL | 19.7472 mL |
| 5 mM | 0.3949 mL | 1.9747 mL | 3.9494 mL |
| 10 mM | 0.1975 mL | 0.9874 mL | 1.9747 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Fitness and inheritance of Metaflumizone resistance in Plutella xylostella
Pestic Biochem Physiol 2017 Jun;139:53-59.PMID:28595922DOI:10.1016/j.pestbp.2017.04.010.
The diamondback moth, Plutella xylostella (L.) has developed resistance to many types of insecticides in the field. To study inheritance and fitness cost of Metaflumizone resistance, a susceptible strain of diamondback moth was continuously selected with Metaflumizone during 37 generations under laboratory conditions. The resistance to Metaflumizone was at a high level (resistance ratios from 250.37 to 1450.47-fold). We investigated a Metaflumizone resistance strain (G27) and a susceptible strain of P. xylostella, using the age-stage, two-sex life table approach. Compared to the susceptible strain, egg duration, the developmental time of the first and second instar larvae, pupae duration, adult preoviposition period (APOP), total preoviposition period (TPOP), egg hatchability, the survival rate of second instar larva and the mean generation time (T) were significantly differences in the resistant strain. The resistant strain had a relative fitness of 0.78. The inheritance of Metaflumizone resistance was also studied by crossing the Metaflumizone resistant and susceptible populations. Results revealed an autosomal and incompletely recessive mode of inheritance for Metaflumizone resistance in the resistant population of P. xylostella. The present study provided useful information for planning potential management strategies to delay development of Metaflumizone resistance in P. xylostella.
Metaflumizone inhibits the honeybee NaV 1 channel by targeting recovery from slow inactivation
FEBS Lett 2017 Dec;591(23):3842-3849.PMID:29105054DOI:10.1002/1873-3468.12897.
Metaflumizone is the latest addition to the armamentarium of the Na+ channel inhibitor insecticide family. We used the Xenopus oocyte expression system and a Markovian model to assess the effect of Metaflumizone on Apis mellifera Na+ channels (AmNaV 1). Our results reveal that Metaflumizone inhibits AmNaV 1 channels by targeting the kinetics of recovery from slow inactivation. Multistate modeling of fast and slow inactivation of the AmNaV 1 channel made it possible to study the effects of Metaflumizone on a set of rate constants underlying the transition between the open and inactivated conformations and provided insights into their specificity. We conclude that the methods we used could be extended to assessing the toxicity of other Na+ channel inhibitor insecticides.
Resistance Risk Evaluated by Metaflumizone Selection and the Effects on Toxicities Over Other Insecticides in Spodoptera exigua (Lepidoptera: Noctuidae)
J Econ Entomol 2019 Sep 23;112(5):2354-2361.PMID:31219572DOI:10.1093/jee/toz171.
Metaflumizone is a novel semicarbazone insecticide. It functions as a sodium channel blocker insecticide (SCBI) with excellent insecticidal activity on most economically important lepidopterous pests. This study assessed the resistance risk of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) to Metaflumizone in the laboratory and the effects of Metaflumizone selection on toxicities to other insecticides. Spodoptera exigua collected from a field population at Huizhou in 2012 were successively challenged by Metaflumizone to evaluate the risk of resistance evolution. Twelve generations of selection increased resistance to Metaflumizone by 3.4-fold and threshold trait analysis revealed that the realized heritability (h2) of this resistance was 0.086. When h2 was equal to 0.086 and 90% of individuals were killed at each generation, LC50 to Metaflumizone increased by 10-fold after 15 generations. The selection by Metaflumizone did not increase the resistance to indoxacarb, chlorantraniliprole, spinosad, methomyl, or endosulfan, suggesting a lack of cross-resistance. However, Metaflumizone challenge upheld the recession of resistance to emamectin benzoate, chlorfluazuron, and tebufenozide. The block of resistance drops by Metaflumizone exposure implied a possible cross-resistance between Metaflumizone and these three insecticides. These results contribute to integrated resistance management of S. exigua.
Do fall armyworm's Metaflumizone resistante populations affect the activity of Trichogramma pretiosum?
Braz J Biol 2021 Oct 11;83:e245273.PMID:34669790DOI:10.1590/1519-6984.245273.
The possible interference of resistant pest's populations to insecticides in natural enemies in the action thas not been clarified yet. Thus, this study aimed to evaluate Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) performance on Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) eggs with resistance frequency to the Metaflumizone over six generations of product exposure. Egg cards (2.0 x 7.0 cm) containing eggs from two populations of S. frugiperda, (resistant to Metaflumizone and the other susceptible), were exposed to T. pretiosum females for 24 hours in free-choice and no-choice testing in three generations (G1, G4, and G6). A completely randomized experimental design was used with 25 replications, each consisting of an egg card (experimental unit) containing 20 eggs. The parameters evaluated were: parasitism (%), emergence (%), sex ratio, number of emerged parasitoids per egg and males/females longevity. ANOVA and Tukey test (P≤ 0.05) were applied on the results. Results showed a reduction in parasitism [41.0% (G1) and 28.4% (G4)], egg emergence (17.5%) and parasitoids/egg [16.2 (G4) and 17.2 (G6)] in eggs originating from the population with resistance frequency. Females emerging from G6 populations eggs without exposure to Metaflumizone had greater longevity (3.5 days more) than the resistant population. The sex ratio and male longevity were not affected. The results indicate a reduction in T. pretiosum activity if S. frugiperda populations have some frequency of resistance to Metaflumizone.
Toxicological properties of Metaflumizone
Vet Parasitol 2007 Dec 15;150(3):190-5.PMID:17933467DOI:10.1016/j.vetpar.2007.08.033.
Metaflumizone is a new insecticide developed for crop protection and urban pest control by BASF. Its mammalian toxicological profile was assessed by conducting multiple toxicity studies in the rat, mouse, and dog, covering all relevant endpoints. Metaflumizone is characterized by very low acute toxicity, is not irritating to the eye or the skin and does not possess a potential to induce skin sensitization. The substance also shows relatively low toxicity following subchronic oral or dermal exposure to mammals. In addition, Metaflumizone demonstrates low toxicological potential following chronic oral exposure to rats, mice, and dogs. Overall, the lowest no observed adverse effect level (NOAEL) is 12mg/(kgday) from the 1-year chronic dog study. In a battery of in vitro and in vivo mutagenicity assays, the weight-of-the-evidence indicates a lack of potential genotoxicity for Metaflumizone. Furthermore, the compound demonstrated a lack of potential oncogenicity in long-term toxicity studies in rats and mice. Results from the rat multi-generation reproductive toxicity study as well as the rat and rabbit developmental toxicity studies indicate that Metaflumizone is not selectively toxic to the offspring or fetus, as compared to the parents. Also, Metaflumizone is not teratogenic in the rat or rabbit. Lastly, no neurotoxicity could be detected in acute and subchronic neurotoxicity studies in rats.