Propineb
(Synonyms: 丙森锌; Zinc propylenebis(dithiocarbamate)) 目录号 : GC34724
Propineb(Zincpropylenebis)是一种广泛用于水果和蔬菜病害的化合物，其具有抗植物真菌疾病的广谱活性。
Cas No.:12071-83-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Propineb (Zinc propylenebis) is a compound widely used in fruit and vegetables cultures, due to its large spectrum of activity against fungal plant diseases[1].
Propineb (Zinc propylenebis) has a moderate to low acute toxicity with a specific goitrogenic effect in rats. long-term exposure, oral or by inhalation is related to carcinogenicity, teratogenicity, malfunction of the reproductive system and malformation of their vitals[2].
[1]. Viviani B, et al. Dithiocarbamate propineb induces acetylcholine release through cytoskeletal actin depolymerization in PC12 cells. Toxicol Lett. 2008 Nov 10;182(1-3):63-8. [2]. Kazos EA, et al. Determination of dithiocarbamate fungicide propineb and its main metabolite propylenethiourea in airborne samples. Chemosphere. 2007 Aug;68(11):2104-10.
| Cas No. | 12071-83-9 | SDF | |
| 别名 | 丙森锌; Zinc propylenebis(dithiocarbamate) | ||
| Canonical SMILES | S=C([S-])NCC(C)NC1=[S][Zn+2][S-]1 | ||
| 分子式 | C5H8N2S4Zn | 分子量 | 289.77 |
| 溶解度 | DMSO : 33.33 mg/mL (93.85 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.451 mL | 17.2551 mL | 34.5101 mL |
| 5 mM | 0.6902 mL | 3.451 mL | 6.902 mL |
| 10 mM | 0.3451 mL | 1.7255 mL | 3.451 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Determination of Propineb in vegetable samples after a coprecipitation strategy for its separation-preconcentration prior to its indirect determination by FAAS
Food Chem 2022 Sep 15;388:133002.PMID:35468462DOI:10.1016/j.foodchem.2022.133002.
In the presented work, a coprecipitation method was developed for separation-preconcentration, and determination of trace quantities of Propineb in vegetable samples. Propineb was coprecipitated by using Al(OH)3. The zinc contents in complex structure of Propineb was determined by flame atomic absorption spectrometry (AAS). The Propineb concentration was calculated by using stoichiometric relationship between the zinc and Propineb. Several parameters including the amount of aluminum(III) as carrier element and hydroxide concentration and sample volume were examined. The effects of matrix ions were also investigated. The preconcentration factor was calculated as 15. The limit of detection (LOD) value for Propineb was calculated as 15.2 μg L-1. The presented coprecipitation procedure was successfully applied to determination of Propineb in vegetable samples.
Dithiocarbamate Propineb induces acetylcholine release through cytoskeletal actin depolymerization in PC12 cells
Toxicol Lett 2008 Nov 10;182(1-3):63-8.PMID:18822360DOI:10.1016/j.toxlet.2008.08.016.
Neurological complications as well as movement disorders are relevant symptoms in animals and humans chronically exposed to dithiocarbamates. Using rat pheochromocytoma cells differentiated by NGF (PC12), we investigated whether Propineb affects acetylcholine (Ach) release and the molecular mechanisms involved. Propineb (0.001-100 nM) dose-dependently increased Ach release from PC12. Thus, 0.001-1 nM propineb-induced Ach release, reaching a maximal effect ( approximately 50%) at 0.1-1 nM. Higher concentrations of Propineb (10-100 nM) caused a progressive disappearance of the effect. Chelation of extra- and intracellular Ca(2+) did not affect Ach release by Propineb, which was prevented by the actin stabilizer jasplakinolide (500 nM). Accordingly, actin depolymerization was observed after exposure of differentiated PC12 to 0.1-1 nM Propineb, a loss of effect was evident at higher concentrations (100 nM), and the effect was Ca(2+)-independent. Disulfiram, a related dithiocarbamate not coordinated with Zn(2+), also depolymerized actin, suggesting the involvement of the organic structure of dithiocarbamates rather than the leakage of Zn(2+). Nevertheless, Propineb did not depolymerize actin in a cell-free system. These data suggest that dithiocarbamates, through the activation of intracellular cascade(s), impair cytoskeletal actin. This effect may contribute to affect synaptic vesicles processing resulting in an impaired cholinergic transmission.
Multiple toxicity of Propineb in developing zebrafish embryos: Neurotoxicity, vascular toxicity, and notochord defects in normal vertebrate development
Comp Biochem Physiol C Toxicol Pharmacol 2021 May;243:108993.PMID:33529709DOI:10.1016/j.cbpc.2021.108993.
A dithiocarbamate (DTC) fungicide, Propineb, affects thyroid function and exerts immunotoxicity, cytotoxicity, and neurotoxicity in humans. Long-term exposure to Propineb is associated with carcinogenicity, teratogenicity, malfunction of the reproductive system, and abnormalities in vital signs during organ development. However, there is no evidence of acute toxicity attributable to Propineb in zebrafish. Therefore, in the present study, we assessed the toxicity of Propineb in zebrafish by studying its adverse effects on embryo development, angiogenesis, and notochord development. Embryos with Propineb exposure developed morphological and physiological defects and in larvae, apoptosis and notochord defects were induced in the early development stage. Transgenic fli1:eGFP zebrafish exposed to Propineb showed abnormal larval development with defects in angiogenesis and deformed vasculature. Propineb induced irreversible damage to the neural development of embryos and neurogenic defects in developing zebrafish in transgenic olig2:dsRED zebrafish. These results show that exposure to Propineb triggers abnormalities in different organ systems of zebrafish and suggests the physiological complexity of the response to Propineb.
Dissipation kinetics and risk assessment of iprovalicarb + Propineb fungicide in tomato under different agroclimates
Environ Sci Pollut Res Int 2021 Jun;28(24):31909-31919.PMID:33616825DOI:10.1007/s11356-021-12919-5.
Multi-location supervised field trials in India were conducted with a combination pesticide formulation (iprovalicarb 5.5% + Propineb 61.25%, 66.75% WP) in tomato to study dissipation behavior at single (iprovalicarb 137.5 g a.i. ha-1 + Propineb 1531.25 g a.i. ha-1) and double (iprovalicarb 275 g a.i. ha-1 + Propineb 3062.5 g a.i. ha-1) dose. The samples were processed using a modified QuEChERS method for iprovalicarb and acid hydrolysis followed by carbon disulfide estimation for Propineb and confirmation of their respective residues by LC-MS/MS and GC-MS. Both the fungicides in tomato fruits obey first-order kinetics irrespective of location and doses. Half-life (t1/2) values at all the four locations ranged from 1.08 to 4.67 days for iprovalicarb and 3.36 to 11.41 days for Propineb in tomato. The Food Safety and Standards Authority of India (FSSAI) has set MRL of 1 mg kg-1 for Propineb, but no MRL is yet fixed for iprovalicarb. Using OECD MRL calculator, the calculated MRL for iprovalicarb and Propineb was found to be 2 and 4 mg kg-1, respectively. The hazard quotient (HQ) < 1, theoretical maximum daily intake (TMDI) < acceptable daily intake (ADI), TMDI < maximum permissible intake (MPI), percent acute hazard index (% aHI) ≤ 1, and percent chronic hazard index (% cHI) < 1 for both the fungicides indicated that the combination formulation will not pose any dietary risk and thus considered safe for human health.
Determination of Propineb and its metabolites propylenethiourea and propylenediamine in banana and soil using gas chromatography with flame photometric detection and LC-MS/MS analysis
J Environ Sci Health B 2018 Mar 4;53(3):153-160.PMID:29227190DOI:10.1080/03601234.2017.1399765.
A sensitive and specific method for the determination of Propineb and its metabolites, propylenethiourea (PTU) and propylenediamine (PDA), using gas chromatography with flame photometric detection (GC-FPD) and LC-MS/MS was developed and validated. Propineb and its metabolite residue dynamics in supervised field trials under Good Agricultural Practice (GAP) conditions in banana and soil were studied. Recovery of Propineb (as CS2), PDA and PTU ranged from 75.3 to 115.4% with RSD (n = 5) of 1.3-11.1%. The limit of quantification (LOQ) of CS2, PDA and PTU ranged from 0.005 to 0.01 mg kg-1, and the limit of detection (LOD) ranged from 0.0015 to 0.0033 mg kg-1. Dissipation experiments showed that the half-life of Propineb in banana and soil ranged from 4.4 to 13.3 days. PTU was found in banana with a half-life of 31.5-69.3 days, while levels of PDA were less than 0.01 mg kg-1 in banana and soil. It has been suggested that PTU is the major metabolite of Propineb in banana. The method was demonstrated to be reliable and sensitive for the routine monitoring of Propineb and its metabolites in banana and soil. It also serves as a reference for the detection and monitoring of dithiocarbamates (DTCs) residues and the evaluation of their metabolic pathway.