Rhodamine 110 (Rhodamine 110 chloride)
(Synonyms: 罗丹明110; Rhodamine 110 chloride; RH110) 目录号 : GC30300
A cationic dye
Cas No.:13558-31-1
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Rhodamine 110 is a green fluorescent cationic dye with excitation and emission maxima of 496 and 520 nm, respectively.1 When incorporated with a hydrolytic substrate (e.g., proteinase or peptidase substrates), it can be used as a highly sensitive detection reagent in fluorescence-based enzyme assays.2 Rhodamine 110 has also been used in a fluorescence quenching method for determining trace nitrite and as a probe for cytochrome P450 activity.3,4
1.Thatte, H.S., Rhee, J.-H., Zagarins, S.E., et al.Acidosis-induced apoptosis in human and porcine heartAnn. Thorac. Surg.77(4)1376-1383(2004) 2.Hug, H., Los, M., Hirt, W., et al.Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cellsBiochemistry38(42)13906-13911(1999) 3.Zhang, X., Wang, H., Fu, N.N., et al.A fluorescence quenching method for the determination of nitrite with Rhodamine 110Spectrochimica Acta.A.Mol.Biomol.Spectrosc.59(8)1667-1772(2003) 4.Yatzeck, M.M., Lavis, L.D., Chao, T.Y., et al.A highly sensitive fluorogenic probe for cytochrome P450 activity in live cellsBioorg. Med. Chem. Lett.18(22)5864-5866(2008)
Animal experiment: | Rats[1] Adult male Sprague-Dawley rats (230±20 g) are used. After the rats recovered, Rhodamine 110 in polyethylene glycol 400 (0.3 and 1 mg/mL) is administered at 3 mg/kg and 10 mg/kg by oral gavage. Blood samples (150 μL) are collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. After each sampling, 100 μL of normal saline is administered via catheter to compensate for the loss of body fluid, and a 50 μL heparin solution (20 IU heparin/mL normal saline) is provided to prevent coagulation. Blood samples are centrifuged at 16,000g for 10 min at 4 °C to obtain plasma, which is stored at -20 °C until analysis. After surgery, Rhodamine 110 in polyethylene glycol 400 (1 mg/mL) is administered intravenously to rats at 3 mg/kg (n=6). A 150 μL blood sample is collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. Then, 100 μL of normal saline is administered via the right jugular vein to compensate for body fluid loss, and 50 μL of a heparin solution (20 IU heparin/mL normal saline) is provided to prevent blood clotting. |
References: [1]. Jiang SH, et al. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats. J Agric Food Chem. 2017 Sep 6;65(35):7797-7804. | |
| Cas No. | 13558-31-1 | SDF | |
| 别名 | 罗丹明110; Rhodamine 110 chloride; RH110 | ||
| Canonical SMILES | O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)O.[Cl-] | ||
| 分子式 | C20H15ClN2O3 | 分子量 | 366.8 |
| 溶解度 | DMSO : 30 mg/mL (81.79 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.7263 mL | 13.6314 mL | 27.2628 mL |
| 5 mM | 0.5453 mL | 2.7263 mL | 5.4526 mL |
| 10 mM | 0.2726 mL | 1.3631 mL | 2.7263 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet