RIP1/RIP3/MLKL activator 1
目录号 : GC67969RIP1/RIP3/MLKL activator 1 (Compound 6i) 是一种有效的抗胶质瘤 (anti-glioma) 药物。RIP1/RIP3/MLKL activator 1 通过激活 RIP1/RIP3/MLKL 通路诱导细胞坏死 (Necroptosis)。 RIP1/RIP3/MLKL activator 1 可透过血脑屏障。
Cas No.:2682850-41-3
Sample solution is provided at 25 µL, 10mM.
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- Purity: >98.00%
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RIP1/RIP3/MLKL activator 1 (Compound 6i) is a potent anti-glioma agent. RIP1/RIP3/MLKL activator 1 induces Necroptosis through RIP1/RIP3/MLKL pathway. RIP1/RIP3/MLKL activator 1 exerts acceptable BBB permeability[1].
RIP1/RIP3/MLKL activator 1 (Compound 6i) (96 h) shows antiproliferative activities in human glioma cell lines[1].
RIP1/RIP3/MLKL activator 1 (0-4 µM, 0-72 h) exhibits remarkable antiproliferative activity for U251 cells in a time- and concentration-dependent manner[1].
RIP1/RIP3/MLKL activator 1 (10 µM, 0-72 h) shows acceptable stability[1].
RIP1/RIP3/MLKL activator 1 (0-2 µM, 24 h) effectively inhibits the migration of U251 cells[1].
RIP1/RIP3/MLKL activator 1 induces necroptosis through RIP1/RIP3/MLKL pathway, and induces mitochondrial depolarization in U251 cells[1].
RIP1/RIP3/MLKL activator 1 could not induce apoptosis in U251 cells[1].
Cell Proliferation Assay[1]
Cell Line: | A172, LN229, U87, U251 and L02 cell lines |
Concentration: | 0-4 µM for U251 cells |
Incubation Time: | 96 h; 24, 48, and 72 h for U251 cells |
Result: | Showed antiproliferative activity with IC50 values of 3.03 ± 0.70, 1.78 ± 0.79, 1.22 ± 0.89, 0.94 ± 0.45, and 0.99 ± 0.46 µM against A172, LN229, U87, U251 and L02 cells, respectively. Time- and concentration-dependently inhibited the growth in U251 cells. |
Western Blot Analysis[1]
Cell Line: | U251 |
Concentration: | 0, 0.5, 1, 2, and 4 µM |
Incubation Time: | 24 or 48 h |
Result: | Concentration-dependently upregulated the expression of p-RIP1, RIP1, p-RIP3, RIP3, p-MLKL, and MLKL at 24 or 48 h. |
RIP1/RIP3/MLKL activator 1 (Compound 6i) (2.50 ng/tail; i.v.; 48 h) inhibits U251 cell proliferation in vivo and exerts acceptable BBB permeability[1].
Animal Model: | Zebrafish wide-type AB strain; 200 CM-DiI labeled U251 cells were transplanted into yolk sac of each wild-type zebrafish embryos at 2 dpf (2 days postfertilization)[1] |
Dosage: | 2.50 ng/tail |
Administration: | Microinjection; 48 h |
Result: | Remarkably reduced the U251 xenografts fluorescence intensity. |
[1]. Yao Feng, et al. Synthesis and biological evaluation of celastrol derivatives as potential anti-glioma agents by activating RIP1/RIP3/MLKL pathway to induce necroptosis. Eur J Med Chem. 2022 Feb 5;229:114070.
Cas No. | 2682850-41-3 | SDF | Download SDF |
分子式 | C43H56N4O3 | 分子量 | 676.93 |
溶解度 | 储存条件 | 4°C, stored under nitrogen | |
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制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.4773 mL | 7.3863 mL | 14.7726 mL |
5 mM | 0.2955 mL | 1.4773 mL | 2.9545 mL |
10 mM | 0.1477 mL | 0.7386 mL | 1.4773 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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