RNGTT Human
目录号 : GP22226RNA Guanylyltransferase And 5'-Phosphatase Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 85.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | RNA Guanylyltransferase And 5'-Phosphatase; CAP1A; RNAGuanylyltransferase And 5-Phosphatase; HCAP1; HCE1; HCE; MRNA-Capping Enzyme; HCAP3; mRNA-capping enzyme. | ||
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHPSMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERNPPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFAQARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGKKESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMDKQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHLSNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIEREIISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDILKWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIECKFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTAASQGQKRKHH LDPDTELMPP PPPKRPRPLT. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | RNGTT protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 7.5), 0.2M NaCl, 40% glycerol, 2mM DTT and 0.1mM PMSF. | ||
RNA Guanylyltransferase And 5'-Phosphatase also known as RNGTT is abifunctional mRNA-capping enzyme which exhibits RNA 5'-triphosphatase activityin the N-terminal section and mRNA guanylyltransferase activity in theC-terminal section. In addition, RNGTTcatalyzes the first two steps of cap formation, through removing thegamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield adiphosphate end, and also by transferring the gmp moiety of GTP to the5'-diphosphate terminus.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.