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Ro 31-9790 (GI4747) Sale

(Synonyms: GI4747) 目录号 : GC33881

Ro 31-9790 (GI4747) 是一种合成金属蛋白酶 (MMP) 抑制剂。

Ro 31-9790 (GI4747) Chemical Structure

Cas No.:145337-55-9

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥2,873.00
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1mg
¥1,665.00
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5mg
¥4,140.00
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Sample solution is provided at 25 µL, 10mM.

Description

Ro 31-9790 is a synthetic metalloproteinase (MMP) inhibitor.

Ro 31-9790 inhibits L-selectin shedding from mouse and human lymphocytes, Jurkat T cells, and human monocytes with an IC50 of 0.3-0.4 μM on these cell types. The IC50 values obtained for Ro 31-9790 are 4.82 ± 0.75 μM, 1.16 ± 0.27 μM, 0.70 ± 0.06 μM, 4.47± 1.27 μM and 0.38 ± 0.05 μM for mouse lymphocyte L-selectin shedding, Jurkat L-selectin shedding, human lymphocyte L-selectin shedding, human monocyte L-selectin shedding and human monocyte TNF-α shedding[1].

[1]. Borland G, et al. Tissue inhibitor of metalloproteinases-3 inhibits shedding of L-selectin from leukocytes. J Biol Chem. 1999 Jan 29;274(5):2810-5.

实验参考方法

Cell experiment:

For mouse and human lymphocytes and Jurkat T cells, 100% inhibition is set as the percentage of cells positive for L-selectin in an untreated sample (after flow cytometric analysis) or the concentration of soluble L-selectin in the supernatant of an untreated cell sample (after ELISA); 0% inhibition is set at the appropriate value for a PMA-treated sample. For results from human monocytes, a different calculation is needed to allow direct comparison of L-selectin and TNF-α shedding. Therefore, in this case 100% inhibition is set as the percentage of cells positive for L-selectin in the presence of PMA+50 μM Ro 31-9790 or the percentage of cells positive for cell surface TNF-α in the presence of LPS+50 μM Ro 31-9790 (doses of Ro 31-9790 which gave maximal inhibition); 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS, respectively. Intermediate percent inhibition is calculated. The IC50 value for inhibitor in each system is defined as the concentration of inhibitor which gave 50% inhibition, where 100% inhibition is set as the percentage of cells positive for L-selectin or cell surface TNF-α in the presence of Phorbol myristate acetate (PMA)+50 μM Ro 31-9790 or Lipopolysaccharide (LPS)+50 μM Ro 31-9790, respectively (doses of Ro 31-9790 which gave maximal inhibition) and 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS alone, respectively[1].

References:

[1]. Borland G, et al. Tissue inhibitor of metalloproteinases-3 inhibits shedding of L-selectin from leukocytes. J Biol Chem. 1999 Jan 29;274(5):2810-5.

化学性质

Cas No. 145337-55-9 SDF
别名 GI4747
Canonical SMILES O=C(N[C@H](C(NC)=O)C(C)(C)C)[C@H](CC(C)C)CC(NO)=O
分子式 C15H29N3O4 分子量 315.41
溶解度 DMSO : 50 mg/mL (158.52 mM; Need ultrasonic (<70°C)) 储存条件 Store at -20°C
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1 mg 5 mg 10 mg
1 mM 3.1705 mL 15.8524 mL 31.7048 mL
5 mM 0.6341 mL 3.1705 mL 6.341 mL
10 mM 0.317 mL 1.5852 mL 3.1705 mL
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