Rose Bengal
(Synonyms: 酸性红 94) 目录号 : GC15691
Rose Bengal是囊泡谷氨酸转运蛋白（VGlut）和囊泡单胺转运体（VMAT）的强效抑制剂，Ki值分别为19nM和64nM。Rose Benga可用作染料、光敏剂，也可用于结肠癌、乳腺癌和卵巢癌的研究。
Cas No.:632-69-9
Sample solution is provided at 25 µL, 10mM.
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Rose Bengal is a potent inhibitor of VGlut and vesicular monoamine transporter (VMAT), with Ki values of 19nM and 64nM, respectively [1]. Rose Bengal can be used as a dye, photosensitizer[2], and can also be used in the research of colon cancer, breast cancer, and ovarian cancer[3][4][5].
Rose Bengal (100-800μM; 4 days) dose-dependently inhibited the growth of UWB and BRCA1 ovarian cancer cells[3]. Rose Bengal (160μM; 60s) significantly enhanced ultrasound-induced injury in sarcoma cells in vitro, with 160μM Rose Bengal increasing the rate of ultrasound-induced cell injury by 2 to 3 times[4]. After treating MCF-7 with Rose Bengal (200-300μM; 24-48h) for 24h, MCF-7 cells undergo morphological changes, including volume reduction and rounding, until the nucleus constitutes the majority of the cell volume[5]. Rose Bengal (25-1000μM; 24h)-treated colon cancer cells expressed distinct hallmarks of immunogenic cell death (ICD), including enhanced expression of calreticulin and heat-shock protein 90 on the cell surface, a decrease in intracellular ATP, and the release of HMGB1[6].
Rose Bengal (300μM; 24h)-treated colon cancer served as a vaccine against subsequent challenge with the same CT26 colon cancer tumor cells, and vaccination with in vitro Rose Bengal-treated cells resulted in slower tumor growth following inoculation with colon cancer cells[6].Intralesional injection of Rose Bengal (50μL 10% Rose Bengal) induces systemic tumor-specific immune responses in mouse models of melanoma and breast cancer[7].
References:
[1]. Ogita K, Hirata K, Bole D G, et al. Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal[J]. Journal of neurochemistry, 2001, 77(1): 34-42.
[2]. Watts L T, Zheng W, Garling R J, et al. Rose Bengal photothrombosis by confocal optical imaging in vivo: a model of single vessel stroke[J]. Journal of Visualized Experiments: JoVE, 2015 (100).
[3]. Koevary S B. Selective toxicity of rose bengal to ovarian cancer cells in vitro[J]. International journal of physiology, pathophysiology and pharmacology, 2012, 4(2): 99.
[4]. Umemura S, Yumita N, Umemura K, et al. Sonodynamically induced effect of rose bengal on isolated sarcoma 180 cells[J]. Cancer chemotherapy and pharmacology, 1999, 43: 389-393.
[5]. Mousavi S H, Tavakkol-Afshari J, Brook A, et al. Direct toxicity of Rose Bengal in MCF-7 cell line: role of apoptosis[J]. Food and chemical toxicology, 2009, 47(4): 855-859.
[6]. Qin J, Kunda N, Qiao G, et al. Colon cancer cell treatment with rose bengal generates a protective immune response via immunogenic cell death[J]. Cell death & disease, 2017, 8(2): e2584-e2584.
[7]. Toomey P, Kodumudi K, Weber A, et al. Intralesional injection of rose bengal induces a systemic tumor-specific immune response in murine models of melanoma and breast cancer[J]. PloS one, 2013, 8(7): e68561.
Rose Bengal是囊泡谷氨酸转运蛋白（VGlut）和囊泡单胺转运体（VMAT）的强效抑制剂，Ki值分别为19nM和64nM[1]。Rose Benga可用作染料、光敏剂[2]，也可用于结肠癌、乳腺癌和卵巢癌的研究[3][4][5]。
Rose Benga（100-800μM；4 days）剂量依赖性地抑制UWB和BRCA1卵巢癌细胞的生长[3]。Rose Benga（160μM；60s）显著增强体外肉瘤细胞的超声诱导损伤，160μM Rose Benga使超声诱导的细胞损伤率增加了2至3倍[4]。用Rose Benga（200-300μM；24h）处理MCF-7细胞24h后，MCF-7细胞发生形态变化，包括体积减小和变圆，直到细胞核占细胞体积的大部分[5]。Rose Benga（25-1000μM；24h）处理的结肠癌细胞表现出免疫原性细胞死亡（ICD）的明显特征，包括细胞表面钙网蛋白和热休克蛋白90表达增强、细胞内ATP减少以及HMGB1释放减少[6]。
Rose Benga（300μM; 24h）处理的结肠癌细胞可作为疫苗抵抗随后相同CT26结肠癌肿瘤细胞对小鼠的攻击，并且用体外Rose Benga处理的细胞进行接种可导致接种结肠癌细胞后的肿瘤生长速度减慢[6]。Rose Benga（50μL 10% Rose Benga）的病灶内注射可在黑色素瘤和乳腺癌小鼠模型中诱导全身肿瘤特异性免疫反应[7]。
| Cell experiment [1]: | |
Cell lines | UWB and BRCA1 ovarian cancer cells |
Preparation Method | UWB and BRCA1 ovarian cancer cells were incubated with 100-800μM Rose Bengal for four days. |
Reaction Conditions | 100-800μM; 4 days |
Applications | Rose Bengal dose-dependently inhibited the growth of UWB and BRCA1 ovarian cancer cells. |
| Animal experiment [2]: | |
Animal models | Colon cancer tumor modle |
Preparation Method | CT26 cells were treated in vitro with Rose Bengal (300μM) for 24h and the cells were harvested and washed with PBS. A total of 1×106 Rose Bengal-treated cells in 100μl PBS were injected subcutaneously into the upper right flank of each mouse, on days −14 and −7, whereas control mice received 100μl injections of PBS. One week after the second vaccination (day 0), 1×106 untreated CT26 cells and 1×106 4T1 murine breast cancer cells were inoculated into the lower right flank and upper left flank, respectively. Mice were killed 2 weeks after the challenge injection and the tumors were excised in their entirety. |
Dosage form | 300μM; 24h |
Applications | Rose Bengal-treated tumors served as a vaccine against subsequent challenge with the same CT26 colon cancer tumor cells, and vaccination with in vitro Rose Bengal-treated cells resulted in slower tumor growth following inoculation with colon cancer cells. |
References: | |
| Cas No. | 632-69-9 | SDF | |
| 别名 | 酸性红 94 | ||
| 化学名 | sodium 2,3,4,5-tetrachloro-6-(2,4,5,7-tetraiodo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate | ||
| Canonical SMILES | IC1=C(C(C(C(C(C([O-])=O)=C2Cl)=C(C(Cl)=C2Cl)Cl)=C3C=C4I)=CC(I)=C1[O-])OC3=C(C4=O)I.[Na+].[Na+] | ||
| 分子式 | C20H2Cl4I4Na2O5 | 分子量 | 1017.64 |
| 溶解度 | <101.76mg/ml in Water; <50.88mg/ml in DMSO | 储存条件 | Store at RT, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9827 mL | 4.9133 mL | 9.8267 mL |
| 5 mM | 0.1965 mL | 0.9827 mL | 1.9653 mL |
| 10 mM | 0.0983 mL | 0.4913 mL | 0.9827 mL |
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动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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