Rosmarinic acid
(Synonyms: 迷迭香酸; Labiatenic acid) 目录号 : GN10648
Rosmarinic acid是一种广泛存在于植物中的酚酯化合物。Rosmarinic acid具有抗病毒、抗菌、抗炎、神经保护、抗癌、抗脂质过氧化、细胞凋亡和抗氧化活性。
Cas No.:20283-92-5
Sample solution is provided at 25 µL, 10mM.
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Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid has antiviral, antibacterial, antiinflammatory, neuroprotective, anticancer, anti-lipid peroxidative, apoptotic,and antioxidant activities[1]. Rosmarinic acid has the ability to block complement fixation, inhibit lipoxygenase and cyclooxygenase activity and inhibit the expression of CCL11 and CCR3 by suppressing the IKK-β activity in NF-κB activation signaling[2].
Rosmarinic Acid (2, 4µg/mL; 24h) Promote NHE1 Expression In Vitro[3].Rosmarinic acid (10-100µg/mL; 12h) inhibited ROS and MMP-1 production in NHDF cells induced by H2O2 and UVA, respectively, without exerted cytotoxicity; and Nitric oxide and inducible nitric oxide synthase (iNOS) productions were inhibited by Rosmarinic acid (10-100µg/mL; 12h) in RAW264.7 mouse macrophage cells induced by combined LPS-IFN-γ[4].
Rosmarinic acid (50, 100mg/kg; po, 8 weeks) mediates reverse cholesterol transport through increased plasma cholesterol uptake and subsequent hepatobiliary excretion and induces lipid metabolism by increasing fatty acid β-oxidation in AMPK-dependent activation of CPT1A, thus modulating lipid accumulation and attenuating hyperlipidemia in HFD-fed mice[5].Rosmarinic acid (100mg/kg; po; 30d) alleviates pancreatic β-cell dysfunction and glucolipotoxicity-mediated oxidative stress during HFD-STZ-induced type 2 diabetes mellitus, perhaps through its antioxidant potential[6].
References:
[1].El Kantar S, Yassin A, Nehmeh B, et al. Deciphering the therapeutical potentials of rosmarinic acid[J]. Scientific Reports, 2022, 12(1): 15489.
[2].Lee J, Jung E, Kim Y, et al. Rosmarinic acid as a downstream inhibitor of IKK‐β in TNF‐α‐induced upregulation of CCL11 and CCR3[J]. British journal of pharmacology, 2006, 148(3): 366-375.
[3]. Jung S W, Park G H, Kim E, et al. Rosmarinic acid, as an NHE1 activator, decreases skin surface pH and improves the skin barrier function[J]. International journal of molecular sciences, 2022, 23(7): 3910.
[4].Pattananandecha T, Apichai S, Julsrigival J, et al. Antioxidant activity and anti-photoaging effects on UVA-irradiated human fibroblasts of rosmarinic acid enriched extract prepared from Thunbergia laurifolia leaves[J]. Plants, 2021, 10(8): 1648.
[5]. Nyandwi J B, Ko Y S, Jin H, et al. Rosmarinic acid exhibits a lipid-lowering effect by modulating the expression of reverse cholesterol transporters and lipid metabolism in high-fat diet-fed mice[J]. Biomolecules, 2021, 11(10): 1470.
[6].Govindaraj J, Sorimuthu Pillai S. Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats[J]. Molecular and cellular biochemistry, 2015, 404: 143-159.
Rosmarinic acid是一种广泛存在于植物中的酚酯化合物。Rosmarinic acid具有抗病毒、抗菌、抗炎、神经保护、抗癌、抗脂质过氧化、细胞凋亡和抗氧化活性[1]。Rosmarinic acid能够阻断补体固定，抑制脂氧合酶和环氧合酶活性，并通过抑制NF-κB活化信号中的IKK-β活性来抑制CCL11和CCR3的表达[2]。
Rosmarinic acid（2、4µg/mL；24h）促进体外 NHE1 表达[3]。Rosmarinic acid （10–100µg/mL；12h）可抑制H2O2和UVA分别诱导的NHDF细胞中的ROS和 MMP-1产生，而不会产生细胞毒性；Rosmarinic acid（10-100µg/mL；12h）抑制了LPS-IFN-γ联合诱导的RAW264.7小鼠巨噬细胞中一氧化氮和诱导型一氧化氮合酶（iNOS）的产生[4]。
Rosmarinic acid（50、100mg/kg；po，8 weeks）通过增加血浆胆固醇摄取和随后的肝胆排泄介导胆固醇的逆向转运，并通过增加AMPK依赖的CPT1A激活中脂肪酸β-氧化来诱导脂质代谢，从而调节脂质积累并减轻HFD喂养小鼠的高脂血症[5]。Rosmarinic acid（100mg/kg；po；30d）可能通过其抗氧化潜力缓解HFD-STZ诱导的2型糖尿病期间胰腺β细胞功能障碍和糖脂毒性介导的氧化应激[6]。
| Cell experiment [1]: | |
Cell lines | Normal human epidermal keratinocytes cells |
Preparation Method | Rosmarinic acid was dissolved in dimethyl sulfoxide (DMSO) and mixed in KBM medium at concentrations of 2µg/mL and 4µg/mL.Normal human epidermal keratinocytes (NHEKs) were cultured in keratinocyte growth medium (KGM) in a 75cm2 flask. Cells were collected by trypsin treatment and diluted with KGM to obtain a final density of 1.5×105 cells/mL; then, 2mL of the cell mixture was inoculated onto a 35mm diameter dish and cultured overnight. KGM was then replaced with KBM, and the cells were cultured for 24h. A test sample was dissolved in KBM and added to the dish. After 24h of incubation, total RNA was isolated according to the standard method. |
Reaction Conditions | R2, 4µg/mL; 24h |
Applications | Rosmarinic Acid Promote NHE1 Expression In Vitro. |
| Animal experiment [2]: | |
Animal models | hyperlipidemia model |
Preparation Method | Male C57BL/6 mice (16–20g, 3 weeks old),Food intake and body weight were checked twice a week, and blood glucose concentrations were evaluated monthly in samples obtained from tails using a blood glucose meter. Mice with a fasting blood glucose (FBG) level ≥150mg/dl were considered hyperglycemic. Once hyperglycemia was achieved, the mice were randomly assigned to the following working groups: (1) the control group; the control group was fed a ND, (2) HFD group, (3) HFD + 50mg/kg rosmarinic acid group, (4) HFD + 100mg/kg rosmarinic acid group, and (5) HFD + 100mg/kg metformin group. Rosmarinic acid and metformin hydrochloride were dissolved in distilled water and administered via gastric gavage once a day for 8 weeks. Once treatment was complete, the mice were fasted overnight, FBG levels were measured, and an oral glucose tolerance test (OGTT) was performed. |
Dosage form | 50, 100mg/kg; po, 8 weeks |
Applications | Rosmarinic acid mediates reverse cholesterol transport through increased plasma cholesterol uptake and subsequent hepatobiliary excretion and induces lipid metabolism by increasing fatty acid β-oxidation in AMPK-dependent activation of CPT1A, thus modulating lipid accumulation and attenuating hyperlipidemia in HFD-fed mice. |
References: | |
| Cas No. | 20283-92-5 | SDF | |
| 别名 | 迷迭香酸; Labiatenic acid | ||
| 化学名 | (2R)-3-(3,4-dihydroxyphenyl)-2-[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxypropanoic acid | ||
| Canonical SMILES | C1=CC(=C(C=C1CC(C(=O)O)OC(=O)C=CC2=CC(=C(C=C2)O)O)O)O | ||
| 分子式 | C18H16O8 | 分子量 | 360.33 |
| 溶解度 | ≥ 100.8 mg/mL in DMSO, ≥ 111.2 mg/mL in EtOH, ≥ 22.84 mg/mL in Water with gentle warming | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7752 mL | 13.8762 mL | 27.7523 mL |
| 5 mM | 0.555 mL | 2.7752 mL | 5.5505 mL |
| 10 mM | 0.2775 mL | 1.3876 mL | 2.7752 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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