Rotenone
(Synonyms: 鱼藤酮) 目录号 : GC16775
Rotenone是一种线粒体电子传递链复合物 I 抑制剂，可通过增强线粒体活性氧的产生来诱导细胞凋亡。
Cas No.:83-79-4
Sample solution is provided at 25 µL, 10mM.
Rotenone is a mitochondrial electron transport chain complex I inhibitor that can induce apoptosis by enhancing the production of mitochondrial reactive oxygen species.
HL-60 cells were treated with rotenone (1 μ m ; 36 h) approximately 50% of HL-60 cells showed chromatin condensation and nuclear fragmentation under confocal microscopy[1].Rotenone(1μM/10 nM/100 nM;24-48h) causes dose-dependent cell death in SK-N-MC neuroblastoma cells[3].
Rotenone (2.5 mg/kg/day) for 4 weeks decreased the number of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the SNpc and signal intensity of TH in the striatum[2]. In the renal ischemia-reperfusion model, Rotenone(200ppm;3weeks) can protect renal tubules, block renal fibrosis, reduce inflammation and apoptosis, and improve mitochondrial abnormalities[4].
References:
[1]. Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, Robinson JP. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25.
[2]. Miyazaki I, Isooka N, Imafuku F, Sun J, Kikuoka R, Furukawa C, Asanuma M. Chronic Systemic Exposure to Low-Dose Rotenone Induced Central and Peripheral Neuropathology and Motor Deficits in Mice: Reproducible Animal Model of Parkinson's Disease. Int J Mol Sci. 2020 May 4;21(9):3254.
[3] Sherer TB, Betarbet R, Testa CM, Seo BB, Richardson JR, Kim JH, Miller GW, Yagi T, Matsuno-Yagi A, Greenamyre JT. Mechanism of toxicity in rotenone models of Parkinson's disease. J Neurosci. 2003 Nov 26;23(34):10756-64.
[4] Zhang W, Sha Y, Wei K, Wu C, Ding D, Yang Y, Zhu C, Zhang Y, Ding G, Zhang A, Jia Z, Huang S. Rotenone ameliorates chronic renal injury caused by acute ischemia/reperfusion. Oncotarget. 2018 May 11;9(36):24199-24208.
Rotenone是一种线粒体电子传递链复合物 I 抑制剂，可通过增强线粒体活性氧的产生来诱导细胞凋亡。
用Rotenone（1μM；36h）处理HL-60细胞后，在共聚焦显微镜下，约50%的HL-60细胞出现染色质凝聚和核碎裂[1]。Rotenone（1μM/10 nM/100 nM；24-48h）导致SK-N-MC神经母细胞瘤细胞发生剂量依赖性细胞死亡[3]。
Rotenone（2.5 mg/kg/day）连续4周可减少黑质前核中酪氨酸羟化酶（TH）阳性多巴胺能神经元的数量和纹状体中TH的信号强度[2]。在肾缺血再灌注模型中，Rotenone（200ppm；3周）可保护肾小管，阻断肾纤维化，减少炎症和细胞凋亡，改善线粒体异常[4]。
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | HL-60 cells were treated with rotenone(1 μM ) for 36h. |
Reaction Conditions | Rotenone :1 μM ;36 h |
Applications | HL-60 cells were treated with rotenone (1 μM ; 36 h) approximately 50% of HL-60 cells showed chromatin condensation and nuclear fragmentation under confocal microscopy. |
| Animal experiment [2]: | |
Animal models | Pakanmori(PD) modle |
Preparation Method | Male C57BL/6J mice were injected subcutaneously with rotenone (2.5 mg/kg/day) for 4 weeks using an osmotic mini pump. |
Dosage form | Rotenone(2.5 mg/kg/day);4 weeks;sc |
Applications | Chronic subcutaneous treatment with a low dose of rotenone (2.5 mg/kg/day) for 4 weeks decreased the number of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the SNpc and signal intensity of TH in the striatum. |
References: [1]. Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, Robinson JP. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25. [2]. Miyazaki I, Isooka N, Imafuku F, Sun J, Kikuoka R, Furukawa C, Asanuma M. Chronic Systemic Exposure to Low-Dose Rotenone Induced Central and Peripheral Neuropathology and Motor Deficits in Mice: Reproducible Animal Model of Parkinson's Disease. Int J Mol Sci. 2020 May 4;21(9):3254. | |
| Cas No. | 83-79-4 | SDF | |
| 别名 | 鱼藤酮 | ||
| 化学名 | (2R,6aR,12aS)-8,9-dimethoxy-2-(prop-1-en-2-yl)-1,2,12,12a-tetrahydrochromeno[3,4-b]furo[2,3-h]chromen-6(6aH)-one | ||
| Canonical SMILES | O=C1[C@H](C2=CC(OC)=C3OC)[C@@H](COC2=C3)OC4=C1C=CC5=C4C[C@H](C(C)=C)O5 | ||
| 分子式 | C23H22O6 | 分子量 | 394.42 |
| 溶解度 | ≥ 77.6mg/mL in DMSO | 储存条件 | Store at 4°C, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5354 mL | 12.6768 mL | 25.3537 mL |
| 5 mM | 0.5071 mL | 2.5354 mL | 5.0707 mL |
| 10 mM | 0.2535 mL | 1.2677 mL | 2.5354 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet