Saccharin 1-methylimidazole (SMI)
(Synonyms: 糖精1-甲基咪唑) 目录号 : GC14808
A chemical activator of DNA and RNA synthesis
Cas No.:482333-74-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Saccharin 1-methylimidazole (SMI) is a DNA and RNA synthesis activator.
Saccharin 1-methylimidazole (SMI) is the saccharin salt of 1-methylimidazole. 1-Methylimidazole or N-methylimidazole is identified as an aromatic heterocyclic organic agent with the formula CH3C3H3N2. 1-Methylimidazole is a colourless liquid that is used as a base, a specialty solvent, and as a precursor to some ionic liquids. 1-Methylimidazole is a fundamental nitrogen heterocycle and 1-methylimidazole mimics for a variety of nucleoside bases as well as histidine and histamine.
In vitro: Saccharin 1-methylimidazole was found to be able to perform very well for DNA and RNA synthesis. In addition, by using 3 minute, 6 minute, and 12 minute coupling times, saccharin 1-methylimidazole was evaluated in RNA synthesis using TBDMS phosphoramidites, and the results found that a 6 minute coupling time for saccharin 1-methylimidazole showed ETT at a 6 minute coupling time. In additio, it was found that longer coupling times for Saccharin 1-methylimidazole could result in even further improvements in RNA coupling efficiencies [1].
In vivo: So far, there is no animal in vivo data reported for saccharin 1-methylimidazole.
Clinical trial: Up to now, saccharin 1-methylimidazole is not used clinically.
Reference:
[1] Russell MA, Laws AP, Atherton JH, Page MI. The mechanism of the phosphoramidite synthesis of polynucleotides. Org Biomol Chem. 2008 Sep 21;6(18):3270-5.
| Cas No. | 482333-74-4 | SDF | |
| 别名 | 糖精1-甲基咪唑 | ||
| 化学名 | 1-methyl-1H-imidazol-1-ium benzo[d]isothiazol-3-olate 1,1-dioxide | ||
| Canonical SMILES | [O-]C1=NS(C2=CC=CC=C21)(=O)=O.C[NH+]3C=CN=C3 | ||
| 分子式 | C11H11N3O3S | 分子量 | 265.29 |
| 溶解度 | DMF: 5mg/mL,DMSO: 5mg/mL,Ethanol: 10mg/mL,PBS (pH 7.2): 10mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7695 mL | 18.8473 mL | 37.6946 mL |
| 5 mM | 0.7539 mL | 3.7695 mL | 7.5389 mL |
| 10 mM | 0.3769 mL | 1.8847 mL | 3.7695 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。