Salannin
目录号 : GC68315Salannin，一种柠檬苦素，具有抗溃疡和杀精活性。Salannin 对革兰氏阳性菌和革兰氏阴性菌均有抗菌活性。
Cas No.:992-20-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Salannin, a limonoid bitter principle of the seed oil of Azadirachta indica, shows antiulcer and spermicidal activities. Salannin displays antibacterial activity towards both Gram-positive and Gram-negative bacteria[1][2].
Salannin (10, 20, 50 mg/kg; p.o.) shows a significant protective activity on Aspirin induced gastric lesions[2].
| Animal Model: | Wistar rats, weighing 200-220 g (acetyl salicylic acid induced gastric lesions)[2] |
| Dosage: | 10, 20 and 50 mg/kg |
| Administration: | P.o. |
| Result: | Significantly and dose dependently inhibited acetyl salicylic acid-induced gastric lesions. |
[1]. Divakar C. Madhu, et al. NTIULCER. ANTIBACTERIAL AND SPERMICIDAL ACTIVITIES OF SALANNIN.
[2]. Mohammad Asif, et al. A Review on Spermicidal Activities of Azadirachta indica. Journal of Pharmacognosy and Phytochemistry.
| Cas No. | 992-20-1 | SDF | Download SDF |
| 分子式 | C34H44O9 | 分子量 | 596.71 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6759 mL | 8.3793 mL | 16.7586 mL |
| 5 mM | 0.3352 mL | 1.6759 mL | 3.3517 mL |
| 10 mM | 0.1676 mL | 0.8379 mL | 1.6759 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Insect antifeedant and growth-regulating activities of Salannin and other c-seco limonoids from neem oil in relation to Azadirachtin
J Chem Ecol 1996 Aug;22(8):1453-61.PMID:24226248DOI:10.1007/BF02027724.
The antifeedant and insect growth-regulating activities of Salannin, nimbin, and 6-deacetylnimbin, in comparison with azadirachtin-A, have been studied againstSpodoptera litura, Pericallia ricini, andOxya fuscovittata. Salannin deterred feeding, delayed molt by increasing larval duration, caused larval and pupal mortalities, and decreased pupal weights in the two lepidopterans. Salannin also caused molt delays and nymphal mortalities inOxya fuscovittata. The role of Salannin and other compounds in conferring bioactivity, along with azadirachtin-A, to neem oil/neem seed extracts is emphasized.
Bioefficacy and mode-of-action of some limonoids of Salannin group from Azadirachta indica A. Juss and their role in a multicomponent system against lepidopteran larvae
J Biosci 2004 Dec;29(4):409-16.PMID:15625397DOI:10.1007/BF02712112.
Biological activities of the Salannin type of limonoids isolated from Azadirachta indica A. Juss were assessed using the gram pod borer Helicoverpa armigera (Hubner) and the tobacco armyworm Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Inhibition of larval growth was concomitant with reduced feeding by neonate and third instar larvae. All three compounds exhibited strong antifeedant activity in a choice leaf disc bioassay with 2.0, 2.3 and 2.8 microg/cm(2) of 3-O-acetyl salannol, salannol and Salannin, respectively deterring feeding by 50% in S. litura larvae. In nutritional assays, all three compounds reduced growth and consumption when fed to larvae without any effect on efficiency of conversion of ingested food (ECI), suggesting antifeedant activity alone. No toxicity was observed nor was there any significant affect on nutritional indices following topical application, further suggesting specific action as feeding deterrents. When relative growth rates were plotted against relative consumption rates, growth efficiency of the H. armigera fed diet containing 3-O-acetyl salannol, salannol or Salannin did not differ from that of starved control larvae (used as calibration curve), further confirming the specific antifeedant action of Salannin type of limonoids. Where the three compounds were co-administered, no enhancement in activity was observed. Non-azadirachtin limonoids having structural similarities and explicitly similar modes of action, like feeding deterrence in the present case, have no potentiating effect in any combination.
Effects of the neem tree compounds azadirachtin, Salannin, nimbin, and 6-desacetylnimbin on ecdysone 20-monooxygenase activity
Arch Insect Biochem Physiol 1997;35(1-2):199-209.PMID:9131784DOI:10.1002/(SICI)1520-6327(1997)35:1/2<199::AID-ARCH18>3.0.CO;2-6.
The effects of azadirachtin, Salannin, nimbin, and 6-desacetylnimbin on ecdysone 20-monooxygenase (E-20-M) activity were examined in three insect species. Homogenates of wandering stage third instar larvae of Drosophila melanogaster, or abdomens from adult female Aedes aegypti, or fat body or midgut from fifth instar larvae of Manduca sexta were incubated with radiolabeled ecdysone and increasing concentrations (from 1 x 10(-8) to 1 x 10(-3) M) of the four compounds isolated from seed kernels of the neem tree, Azadirachta indica. All four neem tree compounds were found to inhibit, in a dose-dependent fashion, the E-20-M activity in three insect species. The concentration of these compounds required to elicit a 50% inhibition of this steroid hydroxylase activity in the three insect species examined ranged from approximately 2 x 10(-5) to 1 x 10(-3).
Comparison of anti-feedant and insecticidal activity of nimbin and Salannin photo-oxidation products with neem (Azadirachta indica) limonoids
Pest Manag Sci 2004 May;60(5):459-64.PMID:15154512DOI:10.1002/ps.834.
Photo-oxidation of the neem limonoids nimbin and Salannin with UV light in the presence of oxygen gives two isomeric lactone products per limonoid, nimbinolide and isonimbinolide, and salanninolide and isosalanninolide, respectively. When compared in insect tests with the important limonoids of neem seeds, azadirachtin, nimbin and Salannin, isonimbinolide and isosalanninolide show activity greater than that of nimbin or Salannin and in some respects show activity approaching that of azadirachtin. The photo-oxidation products were tested for anti-feedant activity and toxicity against larvae of three species of Lepidoptera, Spodoptera littoralis (Boisd), Spodoptera frugiperda (FE Smith) and Helicoverpa armigera (Hübner) and nymphs of the locusts Schistocerca gregaria (Forskål) and Locusta migratoria (L).
Rapid preconcentration method for the determination of azadirachtin-A and -B, nimbin and Salannin in neem oil samples by using graphitised carbon solid phase extraction
Analyst 1999 Jan;124(1):19-21.PMID:10563041DOI:10.1039/a806527f.
A simple and rapid method involving solid phase extraction and liquid chromatography for the determination of azadirachtin-A and -B, nimbin and Salannin at nanogram levels in neem oil samples is presented. The neem oil samples are defatted and the compounds of interest extracted by mixing the sample with hexane and passing the hexane solution through a graphitised carbon black column. After washing the column with 2 ml of hexane, azadirachtin-A and -B, nimbin and Salannin are eluted with 5 ml of acetonitrile and quantified using HPLC with UV detection. The recoveries of azadirachtin-A and -B, nimbin and Salannin in fortified oil samples were 97.4-104.7%. The upper limit of quantification is up to 100 micrograms ml-1 without any additional clean-up and with little interference from lipids during the analysis by HPLC. The method was successfully applied to various neem oil samples collected from different locations in India.