Salbutamol (Albuterol)
(Synonyms: 沙丁胺醇半硫酸盐; Albuterol hemisulfate; AH-3365 hemisulfate) 目录号 : GC31059
Salbutamol (Albuterol)是一种短效的β2-肾上腺素能受体激动剂（IC50 = 8.93μM），用于治疗哮喘和慢性阻塞性肺病（COPD）。
Cas No.:51022-70-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Mouse C2C12 myoblast cells |
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Preparation Method |
Salbutamol (Albuterol sulfate) was dissolved in filtered water and stored as 10-3M aliquots at -20°C. C2C12 cells were plated at the density of 1 × 105 cells/60mm dish. Salbutamol at the final concentration of 10−6M were added to the growth medium (GM) at seeding and the cells were incubated for 7 days with medium renewal every two days. Differentiation was induced two days after seeding by replacing the GM with Differentiation Medium (DM), and the density of the cells was examined on photographs taken with Nikon eclipse TS 100 inverted microscope. |
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Reaction Conditions |
10−6M, 0-7 days |
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Applications |
Salbutamol significantly increased the density of myoblast cells at day 1 compared to the untreated (control) cells. |
| Animal experiment [2]: | |
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Animal models |
Anti-Muscle Specific Kinase (MuSK) myasthenia gravis mouse model |
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Preparation Method |
Salbutamol was dissolved in sterile water (vehicle). Mice were treated with Salbutamol (2, 4 or 8mg/kg/day) infused steadily for 2-weeks via an Alzet minipump that was implanted beneath the skin of the mid back on day 0 of the IgG injections. |
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Dosage form |
2, 4 or 8mg/kg/day; 2 weeks; Alzet minipump injections |
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Applications |
Salbutamol significantly reduced the degree of weight loss and weakness compared to vehicle-treated mice. |
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References: |
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Salbutamol (Albuterol) is a short-acting β2 adrenergic receptor agonist (IC50 = 8.93μM), used for treating asthma and chronic obstructive pulmonary disease (COPD).
Salbutamol (10-6M, 30min) induce accumulation of intracellular cAMP and increase the viability of C2C12 myoblast cells during proliferation stage[1]. Salbutamol (10−6M, 0-7 days) significantly increased the density of myoblast cells at day 1 compared to the untreated (control) cells[1]. Salbutamol (16μM, 24h) significantly induces EMT, migration and invasion by ERK (extracellular signal-regulated kinase) phosphorylation in gastric cancer cells[2].
Salbutamol (2, 4 or 8mg/kg/day; 2 weeks; Alzet minipump injections) significantly reduced the degree of weight loss and weakness compared to vehicle-treated mice in anti-Muscle Specific Kinase (MuSK) myasthenia gravis mouse model[3]. Salbutamol (5mg/kg/day, 2 weeks, orally) can increase tumor growth in nude mice with gastric cancer[4].
References:
[1] Ouali BE, Wang HV. Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro. Biochemistry and biophysics reports. 2021 Jul 1;26:101019.
[2] Lu Y, Zhang Y, Zhao H, et al. Chronic stress model simulated by salbutamol promotes tumorigenesis of gastric cancer cells through β2-AR/ERK/EMT pathway. Journal of Cancer. 2022;13(2):401.
[3] Ghazanfari N, Morsch M, Tse N, et al. Effects of the ß2-adrenoceptor agonist, albuterol, in a mouse model of anti-MuSK myasthenia gravis. PloS one. 2014 Feb 5;9(2):e87840.
[4] Lu Y, Zhang Y, Zhao H, et al. Chronic stress model simulated by salbutamol promotes tumorigenesis of gastric cancer cells through β2-AR/ERK/EMT pathway. J Cancer. 2022 Jan 1;13(2):401-412.
Salbutamol (Albuterol)是一种短效的β2-肾上腺素能受体激动剂（IC50 = 8.93μM），用于治疗哮喘和慢性阻塞性肺病（COPD）。
是一种短效的β2-肾上腺素能受体激动剂（IC50 = 8.93μM），用于治疗哮喘和慢性阻塞性肺病（COPD）。
Salbutamol（10-6M，30min）诱导C2C12成肌细胞增殖期细胞内cAMP蓄积，提高细胞活力[1]。与未处理（对照）细胞相比，Salbutamol（10-6M，0-7天）在第1天显著增加了成肌细胞的密度[1]。Salbutamol（16μM，24h）通过细胞外信号调节激酶（ERK）磷酸化诱导胃癌细胞发生EMT、迁移和侵袭[2]。
在抗肌肉特异性激酶（MuSK）重症肌无力小鼠模型中，Salbutamol（2、4或8mg/kg/d，2周，Alzet微泵注射）显著降低了体重减轻和虚弱的程度[3]。Salbutamol（5mg/kg/d，2周，口服）可促进胃癌裸鼠移植瘤的生长[4]。
| Cas No. | 51022-70-9 | SDF | |
| 别名 | 沙丁胺醇半硫酸盐; Albuterol hemisulfate; AH-3365 hemisulfate | ||
| Canonical SMILES | CC(C)(C)NCC(C1=CC(=C(C=C1)O)CO)O.OS(=O)(=O)O | ||
| 分子式 | C13H21NO3.H2SO4 | 分子量 | 337.39 |
| 溶解度 | ≥ 16.9mg/mL in Water; 4mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9639 mL | 14.8196 mL | 29.6393 mL |
| 5 mM | 0.5928 mL | 2.9639 mL | 5.9279 mL |
| 10 mM | 0.2964 mL | 1.482 mL | 2.9639 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A review of the toxicology of salbutamol (albuterol)
This paper reviews the published toxicology of salbutamol. Salbutamol is a relatively selective beta 2-adrenoreceptor stimulant with rapid, potent bronchodilator activity and only minor inotropic or chronotropic effects. It was not found to be mutagenic. LD50 values and other acute studies indicated low toxicity. Findings published for repeat dose studies were mainly uneventful. Tachycardia and flushing of the skin were observed in dogs. There were several findings peculiar to the rat--growth of the salivary gland, enlargement of the Harderian gland, an increase in colloid in the pituitary, and mesovarian leiomyomas. Increases in heart weights associated with inflammation, hypertrophy of muscle fibres, focal myocardial necrosis and fibrosis were seen in rats. Malformation, in the form of cleft palate, was reported in mice but not in rats or rabbits. These treatment related effects reported for salbutamol are not compound-related but rather are class-related. They are an expression of pharmacological activity brought about by the excessive beta stimulant action of high dosage with the drug.