Salidroside
(Synonyms: 红景天苷; Rhodioloside) 目录号 : GN10127
红景天苷是一种具有多种生物活性的糖苷，具有抗癌、抗氧化、抗衰老、抗糖尿病、抗糖尿病、抗高血压、抗炎、免疫调节等药理作用。
Cas No.:10338-51-9
Sample solution is provided at 25 µL, 10mM.
Salidroside is a tyrosol glycoside primarily found in the roots of Rhodiola species, exhibiting a wide range of biological and pharmacological properties, including anti-cancer, antioxidant, anti-aging, anti-diabetic, anti-hypertensive, anti-inflammatory, and immunomodulatory effects. Salidroside can improve muscle nutrition by increasing the expression of mTOR, p-mTOR, and MyHC[1]. It alleviates cachexia symptoms in a mouse model of cancer cachexia by activating the mTOR signaling pathway[1]. Furthermore, salidroside protects dopaminergic neurons by enhancing PINK1/Parkin-mediated mitophagy[2]. Its anti-cancer activity is primarily due to the inhibition of the PI3K/AKT, JAK/STAT, and MEK/ERK pathways, as well as the activation of cell apoptosis and autophagy[3].
In vitro, salidroside significantly increases the expression of mTOR, p-mTOR, and MyHC in C2C12 myotubes and can effectively rescue the downregulation of mTOR, p-mTOR, and MyHC expression induced by tumor necrosis factor-alpha[1]. Salidroside (10, 25, and 50µM) significantly prevents MPP+-induced cytotoxicity and reverses the reduction in dopamine, HVA, and DOPAC levels induced by MPTP in the striatum[2]. Additionally, salidroside (100µM) inhibits the activity of prolyl endopeptidase (PEP), an enzyme associated with learning and memory, by 10.6±1.9%[4].
In vivo, salidroside (120 mg/kg/day; i.p.) significantly maintains fat mass and increases lean body mass, especially the mass of the gastrocnemius muscle, indicating a notable improvement in the main features of cancer-associated cachexia in mice[1]. Salidroside (10/20/40 mg/kg/day) also induces a mouse model of testicular damage[5].
References:
[1] Chen X, et al. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activatingmTOR signalling. J Cachexia Sarcopenia Muscle. 2016 May;7(2):225-32.
[2]Li R, et al. Salidroside Protects Dopaminergic Neurons by Enhancing PINK1/Parkin-Mediated Mitophagy. Oxid Med Cell Longev. 2019 Sep 10; 2019: 9341018.
[3] Zhifeng Z , Jing H , Jizhou Z ,et al.Pharmacological activities, mechanisms of action, and safety of salidroside in the central nervous system[J].Drug Design Development & Therapy, 2018, 12:1479-1489.
[4] Fan W, et al. Prolyl endopeptidase inhibitors from the underground part of Rhodiola sachalinensis. Chem Pharm Bull (Tokyo). 2001 Apr;49(4):396-401.
[5] Wang Z, et al. Salidroside Ameliorates Furan-Induced Testicular Inflammation in Relation to the Gut-Testis Axis and Intestinal Apoptosis. J Agric Food Chem. 2023 Nov 9.
红景天苷是一种主要存在于红景天属植物根中的酪醇糖苷。它具有多种生物学和药理学特性，如抗癌，抗氧化，抗衰老，抗糖尿病，抗糖尿病，抗高血压，抗炎，免疫调节等。 红景天苷可以通过增加mTOR，p-mTOR和MyHC表达来改善肌肉营养[1]。红景天苷可通过激活 mTOR 信号缓解肿瘤恶病质小鼠模型中的恶病质症状[1]。红景天苷还能通过增强 PINK1/Parkin 介导的线粒体自噬来保护多巴胺能神经元[2]。红景天苷对PI 3 k/AKT、JAK/ STAT和MEK/ERK通路的抑制以及对细胞凋亡和自噬的激活是其抗癌活性的主要原因[3]。
在体外，红景天苷不仅能明显增加C2C12肌管中mTOR、p-mTOR和MyHC的表达，而且能有效挽救肿瘤坏死因子-α诱导的mTOR、p-mTOR和MyHC表达下调[1]。红景天苷(10、25和50 μM)显著防止了MPP +诱导的细胞毒性。红景天苷还逆转了纹状体中MPTP诱导的多巴胺、HVA和DOPAC水平的降低[2]。此外，红景天苷(100 μM) 抑制脯氨酰内肽酶 (PEP) 活性 (10.6±1.9%)，该酶被认为与学习和记忆有关[4]。
在体内，红景天苷(120 mg/kg/day; i.p.)能显着地保持脂肪量并增加无肿瘤体重，尤其是腓肠肌的质量，这表明红景天苷可以明显改善小鼠CAC的主要特征[1]。红景天苷(10/20/40 mg/kg/day) 还可诱导的小鼠睾丸损伤模型[5]。
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Cell experiment [1]: |
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Cell lines |
MN9D cells |
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Preparation method |
MN9D cells were cultured in RPMI medium with 10% FBS in a humidified atmosphere incubator of 5% CO2 at 37°C. The cells were pretreated with Salidroside(10, 25, and 50 μM) for 24 h and incubated with 200 μM of MPP+ for an additional 24 h based on a previous dose-effect study |
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Reaction Conditions |
0, 25, 50 μM; 24 h |
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Applications |
200 μM of MPP+ remarkably reduced the cell viability, and Salidroside (10, 25, and 50 μM) markedly prevented the cell toxicity induced by MPP+. |
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Animal experiment [2]: |
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Animal models |
BABL/c and C57BL/6 mice |
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Preparation method |
BABL/c mice were inoculated with CT-26 cells (1.0×106/100 μL), while C57BL/6 mice with LLC cells (7×105/100 μL), both of them were injected s.c. into the right flank. Nine days after inoculation, tumours were palpable (about 5 mm in diameter) in BABL/c and C57BL/6 mice, respectively. Treatments were given i.p. with the control group, salidroside (60 mg/kg/day), and salidroside (120 mg/kg/day) for 25 days in BABL/c tumour model, while for 20 days in C57BL/6 model. |
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Dosage form |
60 mg/kg/day、120 mg/kg/day; i.p. |
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Applications |
salidroside had an effective anti-cachexia activity in CAC models in vivo, including increasing quality of life and prolonging survival time. |
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References: [1]Li R, et al. Salidroside Protects Dopaminergic Neurons by Enhancing PINK1/Parkin-Mediated Mitophagy. Oxid Med Cell Longev. 2019 Sep 10; 2019: 9341018. [2] Chen X, et al. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activatingmTOR signalling. J Cachexia Sarcopenia Muscle. 2016 May;7(2):225-32. |
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| Cas No. | 10338-51-9 | SDF | |
| 别名 | 红景天苷; Rhodioloside | ||
| 化学名 | (2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-[2-(4-hydroxyphenyl)ethoxy]oxane-3,4,5-triol | ||
| Canonical SMILES | C1=CC(=CC=C1CCOC2C(C(C(C(O2)CO)O)O)O)O | ||
| 分子式 | C14H20O7 | 分子量 | 300.3 |
| 溶解度 | ≥ 30mg/mL in DMSO,≥100 mg/mL in Water.Measured | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.33 mL | 16.65 mL | 33.3 mL |
| 5 mM | 0.666 mL | 3.33 mL | 6.66 mL |
| 10 mM | 0.333 mL | 1.665 mL | 3.33 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet