Salmeterol-d3
(Synonyms: 沙美特罗-D3) 目录号 : GC48067An internal standard for the quantification of salmeterol
Cas No.:497063-94-2
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Salmeterol-d3 is intended for use as an internal standard for the quantification of salmeterol by GC- or LC-MS. Salmeterol is a long-acting β2-adrenergic receptor agonist (β2-AR; EC50s = 0.79, 63.1, and 9.4 nM for β2-, β1-, and β3-ARs, respectively).1 It inhibits electrically-stimulated contraction of isolated guinea pig trachea strips (EC50 = 2.51 nM) and histamine-induced bronchoconstriction in guinea pigs via aerosol administration of doses ranging from 0.12 to 12 mM.2 Salmeterol binds to an exosite domain of β2-adrenergic receptors, producing a slow onset of action and prolonged activation.3 Formulations containing salmeterol have been used in the treatment of asthma, including exercise-induced asthma, and chronic obstructive pulmonary disease.
1.Procopiou, P.A., Barrett, V.J., Ford, A.J., et al.The discovery of long-acting saligenin β2 adrenergic receptor agonists incorporating a urea groupBioorg. Med. Chem.19(20)6026-6032(2011) 2.Ball, D.I., Brittain, R.T., Coleman, R.A., et al.Salmeterol, a novel, long-acting β2-adrenoceptor agonist: Characterization of pharmacological activity in vitro and in vivoBr. J. Pharmacol.104(3)665-671(1991) 3.Isin, B., Estiu, G., Wiest, O., et al.Identifying ligand binding conformations of the β2-adrenergic receptor by using its agonists as computational probesPLoS One7(12)e50186(2012)
Cas No. | 497063-94-2 | SDF | |
别名 | 沙美特罗-D3 | ||
Canonical SMILES | OC([2H])([2H])C1=CC(C(O)([2H])CNCCCCCCOCCCCC2=CC=CC=C2)=CC=C1O | ||
分子式 | C25H34D3NO4 | 分子量 | 418.6 |
溶解度 | DMSO: 100 mM,Ethanol: 50 mM | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.3889 mL | 11.9446 mL | 23.8892 mL |
5 mM | 0.4778 mL | 2.3889 mL | 4.7778 mL |
10 mM | 0.2389 mL | 1.1945 mL | 2.3889 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。