Sapropterin dihydrochloride (6R-BH4 dihydrochloride)
(Synonyms: 盐酸沙丙蝶呤; (6R)-BH4 dihydrochloride; (6R)-Tetrahydro-L-biopterin dihydrochloride) 目录号 : GC32438
A cofactor for production of aromatic amino acids, neurotransmitters, and nitric oxide
Cas No.:69056-38-8
Sample solution is provided at 25 µL, 10mM.
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(6R)-5,6,7,8-tetrahydro-L-Biopterin (hydrochloride) (BH4) is a cofactor that, in the presence of enzyme site iron, binds to phenylalanine hydroxylase, tryptophan or tyrosine hydroxylase, and nitric oxide synthase (NOS), to facilitate the production of aromatic amino acids, neurotransmitters, and nitric oxide (NO), respectively.1,2,3 It is formed de novo from GTP with GTP cyclohydrolase-1 (GCH1) catalyzing the rate limiting conversion of GTP to 7,8-dihydroneopterin (NH2TP) followed by subsequent processing by TS and SPR to convert NH2TP to BH4.4,3 BH4 acts as a radical-trapping antioxidant that inhibits phospholipid oxidation in lipid membranes.4 It inhibits IKE- or RSL3-induced ferroptosis in HT-1080 cells (EC50s = 21 and 69 ?M), as well as ferroptosis induced by knockout of glutathione peroxidase (Gpx4-/-) in immortalized mouse fibroblasts.3 BH4 also reduces RLS3-induced lipid peroxidation in murine fibroblasts and HT-1080 cells when used at a concentration of 50 ?M.
1.Kappock, T.J., and Caradonna, J.P.Pterin-dependent amino acid hydroxylasesChem. Rev.96(7)2659-2756(1996) 2.Mayer, B., and Werner, E.R.In search of a function for tetrahydrobiopterin in the biosynthesis of nitric oxideN.-S. Arch. Pharmacol.351(5)453-463(1995) 3.Kraft, V.A.N., Bezjian, C.T., Pfeiffer, S., et al.GTP cyclohydrolase 1/tetrahydrobiopterin counteract ferroptosis through lipid remodelingACS Cent. Sci.6(1)41-53(2020) 4.Soula, M., Weber, R.A., Zilka, O., et al.Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducersNat. Chem. Biol.16(12)1351-1360(2020)
| Cas No. | 69056-38-8 | SDF | |
| 别名 | 盐酸沙丙蝶呤; (6R)-BH4 dihydrochloride; (6R)-Tetrahydro-L-biopterin dihydrochloride | ||
| Canonical SMILES | O=C1N=C(N)NC2=C1N[C@@H]([C@@H](O)[C@@H](O)C)CN2.[H]Cl.[H]Cl | ||
| 分子式 | C9H17Cl2N5O3 | 分子量 | 314.17 |
| 溶解度 | DMSO : 63mg/mL, Water : 34 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.183 mL | 15.915 mL | 31.8299 mL |
| 5 mM | 0.6366 mL | 3.183 mL | 6.366 mL |
| 10 mM | 0.3183 mL | 1.5915 mL | 3.183 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet