SCF Human, Sf9
目录号 : GP20925Stem Cell Factor Human Recombinant, Sf9
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 98.0% as determined by analysis by SDS-PAGE. | Source | Sf9, Insect Cells. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250. | ||
| Solubility | It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml. | ||
| Formulation | The protein is supplied in 1xPBS, pH 7.4. | ||
Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.
Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.