Senecionine (Senecionan-11,16-dione, 12-hydroxy-)
(Synonyms: 千里光宁,Senecionan-11,16-dione, 12-hydroxy-; Aureine; Senecionin) 目录号 : GC30459
A pyrrolizidine alkaloid with hepatotoxic properties
Cas No.:130-01-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Animal experiment: |
Rats[1]Swiss albino rats weighing 155-175 g are incubated for 3 consecutive days with seneciphylline and senecionine at a daily dose of 40 or 80 mg/kg body wt. Control animals received normal saline only. The animals are killed 24 h after the last dose. The livers are removed, weighed and then homogenized in 1.15% KCI, 0.02 HEPES (pH 7.4)[1]. |
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References: [1]. Wang C, et al. The comparative pharmacokinetics of two pyrrolizidine alkaloids, senecionine and adonifoline, and their main metabolites in rats after intravenous and oral administration by UPLC/ESIMS. Anal Bioanal Chem. 2011 Jul;401(1):275-87. |
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Senecionine is a pyrrolizidine alkaloid that has been found in S. vulgaris and has hepatotoxic properties.1 It is metabolized by the cytochrome P450 (CYP) isoform CYP3A in the liver to the detoxification product senecionine N-oxide and reactive metabolites including dehydropyrrolizidine alkaloids and dehydrotetronecine.2,3 Senecionine (20 μM) induces mitochondrial depolarization and fragmentation in primary cultured mouse hepatocytes and increases apoptosis in a concentration-dependent manner.2 In rats, senecionine (35 mg/kg, p.o.) induces liver injury, increases serum levels of bilirubin and various bile acids, including taurocholic acid, glycocholic acid, and deoxycholic acid, and increases the activity of alanine aminotransferase and aspartate aminotransferase in serum.1 Senecionine-induced hepatotoxicity is associated with lipid peroxidation and glutathione depletion.
1.Xiong, A., Yang, F., Fang, L., et al.Metabolomic and genomic evidence for compromised bile acid homeostasis by senecionine, a hepatotoxic pyrrolizidine alkaloidChem. Res. Toxicol.27(5)775-786(2014) 2.Yang, X., Wang, H., Ni, H.-M., et al.Inhibition of Drp1 protects against senecionine-induced mitochondria-mediated apoptosis in primary hepatocytes and in miceRedox Biol.12264-273(2017) 3.Miranda, C.L., Reed, R.L., Guengerich, F.P., et al.Role of cytochrome P450IIIA4 in the metabolism of the pyrrolizidine alkaloid senecionine in human liverCarcinogenesis12(3)515-519(1991)
| Cas No. | 130-01-8 | SDF | |
| 别名 | 千里光宁,Senecionan-11,16-dione, 12-hydroxy-; Aureine; Senecionin | ||
| Canonical SMILES | O=C1O[C@@]2([H])[C@]3([H])N(CC=C3COC([C@](C)(O)[C@H](C)C/C1=C/C)=O)CC2 | ||
| 分子式 | C18H25NO5 | 分子量 | 335.39 |
| 溶解度 | DMF: 5 mg/ml,DMSO: 2 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): 0.1 mg/ml | 储存条件 | Store at 2-8°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9816 mL | 14.908 mL | 29.816 mL |
| 5 mM | 0.5963 mL | 2.9816 mL | 5.9632 mL |
| 10 mM | 0.2982 mL | 1.4908 mL | 2.9816 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。