Serotonin HCl
(Synonyms: 5-羟基色胺盐酸盐; 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride) 目录号 : GC14843
Serotonin HCl是一种神经递质，是介导人体多种中枢和外周功能的关键信使。
Cas No.:153-98-0
Sample solution is provided at 25 µL, 10mM.
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Serotonin HCl a neurotransmitter, is a key messenger that mediates several central and peripheral functions in the human body[1]. Serotonin HCl is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 µM[2].
In vitro, Serotonin HCl (1 nmol/L-10 micromol/L) caused vasoconstriction in a concentration-dependent manner[3]. In vitro, 10 μM Serotonin HCl proliferates colon cancer cells through enhancing YAP (Yes-associated protein) expression[1]. In vitro, for left lower lobe of lungs isolated from the dog, infusion of Serotonin HCl (100 micrograms/min) increased the pulmonary pre- and post-capillary resistances, but did not change the filtration coefficient[4].
In vivo, topical Serotonin HCl (1-10 nmols) or the Serotonin HCl1/7 receptor agonist, 5-carboxamidotryptamine (10-30 nM), dilated third- and fourth-order arterioles, responses that were abolished by 1 μM SB269970, a selective Serotonin HCl7 receptor antagonist[5].
References:
[1] Yu H, et al. Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression. Cell Commun Signal. 2023 Apr 12;21(1):75.
[2] Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25.
[3] Tanaka N, et al. Both 5-hydroxytryptamine 5-HT2A and 5-HT1B receptors are involved in the vasoconstrictor response to 5-HT in the human isolated internal thoracic artery. Clin Exp Pharmacol Physiol. 2008 Jul;35(7):836-40.
[4] Sumita T, et al. 5-Hydroxytryptamine receptor subtypes participating in pulmonary edema in dogs. Eur J Pharmacol. 1989 May 2;164(1):69-75.
[5] Jackson WF, et al. 5-HT7 receptors mediate dilation of rat cremaster muscle arterioles in vivo.Microcirculation. 2023 Aug;30(5-6):e12808.
Serotonin HCl是一种神经递质，是介导人体多种中枢和外周功能的关键信使[1]。
在体外，Serotonin HCl（1 nmol/L-10 micromol/L）以浓度依赖的方式引起血管收缩[2]。在体外，10 μM 5-HT通过增强YAP（Yes-associated protein）的表达来增殖结肠癌细胞[1]。在体外，对于从狗身上分离的左肺下叶，输注Serotonin HCl（100微克/分钟）会增加肺毛细血管前和毛细血管后的阻力，但不会改变过滤系数[3]。
在体内，局部Serotonin HCl（1-10 nmols）或Serotonin HCl 1/7受体激动剂5-甲酰胺色氨酸（10-30 nM）扩张第三和第四级小动脉，反应被1μM SB269970（一种选择性Serotonin HCl 7受体拮抗剂）消除[4]。
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Cell experiment: |
For macrophage culture, peritoneal cavity cells are collected from WT and IL-13-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×106 cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin hydrochloride (10-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system[2]. |
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Animal experiment: |
Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13-/- mice are injected subcutaneously with 100 mg/kg of Serotonin hydrochloride (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)[2]. |
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References: [1]. Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25. |
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| Cas No. | 153-98-0 | SDF | |
| 别名 | 5-羟基色胺盐酸盐; 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride | ||
| 化学名 | 3-(2-aminoethyl)-1H-indol-5-ol;hydrochloride | ||
| Canonical SMILES | C1=CC2=C(C=C1O)C(=CN2)CCN.Cl | ||
| 分子式 | C10H12N2O.HCl | 分子量 | 212.68 |
| 溶解度 | DMF: 16 mg/ml,DMSO: 10 mg/ml,Ethanol: 1.2 mg/ml,PBS (pH 7.2): 5 mg/ml | 储存条件 | Store at 2-8°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7019 mL | 23.5095 mL | 47.019 mL |
| 5 mM | 0.9404 mL | 4.7019 mL | 9.4038 mL |
| 10 mM | 0.4702 mL | 2.3509 mL | 4.7019 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet