SERPINB2 Human
目录号 : GP24539Serpin 肽酶抑制剂，进化枝 B 成员 2 人类重组
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Serpin Peptidase Inhibitor Clade B (Ovalbumin) Member 2; Serine (Or Cysteine) Proteinase Inhibitor Clade B (Ovalbumin) Member 2; Placental Plasminogen Activator Inhibitor; Plasminogen Activator Inhibitor Type II (Arginine-Serpin); PAI2; PLANH2; Monocyte Arg-Serpin; Serpin B2; HsT1201; PAI. | ||
| Amino Acid Sequence | MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP | ||
| Solubility | It is recommended to reconstitute the lyophilized SERPINB2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized SERPINB2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution SERPINB2 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||
| Biological Activity | The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C . Half maximal inhibition against 1.0 µg/ml of single chain tPA was at a concentration of 1.0µg/ml. | ||
| Formulation | Lyophilized from a 0.2µm filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl, 1mM Cysteine, with 5% Trehalose. | ||
SERPINB2 is an inhibitory serpin produced primarily in keratinocytes, stimulated monocytes, and placental trophoblasts. SERPINB2 is found primarily as a 47 kDa non-glycosylated intracellular protein that is induced to be secreted as 60 kDa glycoprotein. The glycosylated and unglycosylated SERPINB2 are similarly effective as inhibitors of uPA, the only proven physiological target of SERPINB2.
The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C . Half maximal inhibition against 1.0 µg/ml of single chain tPA was at a concentration of 1.0µg/ml.
Lyophilized SERPINB2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution SERPINB2 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.