SIH
(Synonyms: 2-异丙基噻吨酮) 目录号 : GC48894
An iron chelator
Cas No.:495-84-1
Sample solution is provided at 25 µL, 10mM.
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SIH is a lipophilic cell-permeable iron chelator.1,2 It stimulates iron release from, and inhibits iron uptake by, SK-N-MC human neuroblastoma cells when used at a concentration of 25 µM.3 SIH inhibits cumene hydroperoxide-induced membrane lipid peroxidation but not ABAP-induced formation of cytosolic peroxyl radicals in HepG2 human hepatocellular carcinoma cells (IC50s = 0.833 and >4,000 µM, respectively).1 It inhibits hydrogen peroxide-induced mitochondrial membrane depolarization in H9c2 rat cardiomyocytes (EC50 = 0.8 µM).2 SIH (1.0 mg/kg) completely prevents mortality in a rabbit model of cardiotoxicity induced by chronic administration of daunorubicin .4
1.Hofer, T., JØrgensen, T.Ø., and Olsen, R.L.Comparison of food antioxidants and iron chelators in two cellular free radical assays: Strong protection by luteolinJ. Agric. Food Chem.62(33)8402-8410(2014) 2.šim?nek, T., Boer, C., Bouwman, R.A., et al.SIH—a novel lipophilic iron chelator—protects H9c2 cardiomyoblasts from oxidative stress-induced mitochondrial injury and cell deathJ. Mol. Cell. Cardiol.39(2)345-354(2005) 3.Wu, Z., Palanimuthu, D., Braidy, N., et al.Novel multifunctional iron chelators of the aroyl nicotinoyl hydrazone class that markedly enhance cellular NAD+ /NADH ratiosBr. J. Pharmacol.177(9)1967-1987(2019) 4.št?rba, M., PopelovÁ, O., šim?nek, T., et al.Iron chelation-afforded cardioprotection against chronic anthracycline cardiotoxicity: A study of salicylaldehyde isonicotinoyl hydrazone (SIH)Toxicology235(3)150-166(2007)
| Cas No. | 495-84-1 | SDF | |
| 别名 | 2-异丙基噻吨酮 | ||
| Canonical SMILES | O=C(C1=CC=NC=C1)NN=CC2=C(O)C=CC=C2 | ||
| 分子式 | C13H11N3O2 | 分子量 | 241.2 |
| 溶解度 | DMF: 10 mg/ml,DMF:PBS (pH 7.2) (1:3): 0.23 mg/ml,DMSO: 5 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.1459 mL | 20.7297 mL | 41.4594 mL |
| 5 mM | 0.8292 mL | 4.1459 mL | 8.2919 mL |
| 10 mM | 0.4146 mL | 2.073 mL | 4.1459 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet