SN50
目录号 : GC31642
SN50， NF-κB细胞通透性抑制肽。
Cas No.:213546-53-3
Sample solution is provided at 25 µL, 10mM.
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SN50, the NF-κB cell permeable inhibitory peptide. It is comprised of the hydrophilic region of the signal peptide of Kaposi fibroblast growth factor as a membrane translocating motif and a nuclear localization sequence derived from the p50 subunit of NF-κB[1].
SN50(20 µg/ml; 12 hs) could induce the microglia differentiation into M2 type through NF-κB pathway. The SN50-induced microglia can protect neurons from hypoxic injury[2]. SN50 (25 µg/ml; 24 hs) could induce robust differentiation of GSCs, and the differentiated daughter cells lose the neuroglobular motility, invasiveness and treatment insensitivity tumorigenicity of tumor stem cells[4].
SN50(100µl,10,30,60µg/ml; i.p.;1h) attenuated alveolar hypercoagulation and fibrinolysis inhibition in ARDS via inhibition of NF-κB p65 translocation[5].SN50(1µM) protected against LPS-induced lung injury in isolated rat lung by inhibiting NF-κB nuclear translocation[6]. Topical SN50 (10µM; 12 days) suppressed nuclear factor-κB activation in local cells and reduced the incidence of epithelial defects/ulceration in C57BL/6 mice healing corneas[7].
References:
[1]. Lin YZ, Yao SY,et al. Inhibition of nuclear translocation of transcription factor NF kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence. J Biol Chem. 1995;270(24):14255-8.
[2]. Chian CF, Chiang CH, et,al. Inhibitor of nuclear factor-κB, SN50, attenuates lipopolysaccharide-induced lung injury in an isolated and perfused rat lung model. Transl Res. 2014 Mar;163(3):211-20. doi: 10.1016/j.trsl.2013.10.002. Epub 2013 Oct 12. PMID: 24646628.
[3]. Zhang L, Ren X, et,al. The NFκB inhibitor, SN50, induces differentiation of glioma stem cells and suppresses their oncogenic phenotype. Cancer Biol Ther. 2014 May;15(5):602-11. doi: 10.4161/cbt.28158. Epub 2014 Feb 20. PMID: 24557012; PMCID: PMC4026083.
[4]. Zhao K, Zhu BS, et,al. SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901. Arch Med Sci. 2013 Dec 30;9(6):990-8. doi: 10.5114/aoms.2013.39790. Epub 2013 Dec 26. PMID: 24482641; PMCID: PMC3902720.
[5]. Wu Y, Wang Y, et,al.SN50 attenuates alveolar hypercoagulation and fibrinolysis inhibition in acute respiratory distress syndrome mice through inhibiting NF-κB p65 translocation. Respir Res. 2020 May 27;21(1):130. doi: 10.1186/s12931-020-01372-6. PMID: 32460750; PMCID: PMC7251840.
[6]. Chian CF, Chiang CH, et,al. Inhibitor of nuclear factor-κB, SN50, attenuates lipopolysaccharide-induced lung injury in an isolated and perfused rat lung model. Transl Res. 2014 Mar;163(3):211-20. doi: 10.1016/j.trsl.2013.10.002. Epub 2013 Oct 12. PMID: 24646628.
[7]. Saika S, Miyamoto T, et,al. Therapeutic effect of topical administration of SN50, an inhibitor of nuclear factor-kappaB, in treatment of corneal alkali burns in mice. Am J Pathol. 2005 May;166(5):1393-403. doi: 10.1016/s0002-9440(10)62357-7. PMID: 15855640; PMCID: PMC1606394.
| Cell experiment [1]: | |
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Cell lines |
Glioma stem-like cells GSC11 and GSC23 cells |
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Preparation Method |
Cells were cultured in medium containing 2% B-27, 20 ng/mL EGF, and 20 ng/mL β-FGF in the presence or absence of SN50 (25 µg/mL) for twenty-four hours. |
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Reaction Conditions |
25 µg/ml; 24 hours |
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Applications |
SN50 can induce GSC differentiation. |
| Animal experiment [2]: | |
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Animal models |
Male Balb/c mice(8-12 weeks) |
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Preparation Method |
Mice was made by 50 µl of lipopolysaccharide (LPS) (4 mg/ml) inhalation. Mice were intraperitoneally injected with different does of SN50 1 h before LPS inhalation. |
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Dosage form |
100µl,10,30,60µg/ml; i.p.;1h |
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Applications |
SN50 inhibited expressions of TF and PAI-1 in mRNA and in protein levels in LPS-induced acute respiratory distress syndrome (ARDS) mice. |
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References: [1]. Zhang L, Ren X, et,al. The NFκB inhibitor, SN50, induces differentiation of glioma stem cells and suppresses their oncogenic phenotype. Cancer Biol Ther. 2014 May;15(5):602-11. doi: 10.4161/cbt.28158. Epub 2014 Feb 20. PMID: 24557012; PMCID: PMC4026083. | |
| Cas No. | 213546-53-3 | SDF | |
| Canonical SMILES | Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro | ||
| 分子式 | C129H230N36O29S | 分子量 | 2781.5 |
| 溶解度 | Water : ≥ 50 mg/mL (17.98 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.3595 mL | 1.7976 mL | 3.5952 mL |
| 5 mM | 0.0719 mL | 0.3595 mL | 0.719 mL |
| 10 mM | 0.036 mL | 0.1798 mL | 0.3595 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet