sodium 4-pentynoate
(Synonyms: 4-戊炔酸钠) 目录号 : GC114804-戊烯酸钠是一种炔基乙酸类似物,可以通过生物合成机制代谢结合到细胞蛋白上,用于分析不同细胞类型中的乙酰化蛋白。
Cas No.:101917-30-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Sodium 4-pentynoate is an alkynylacetate analogue, which could be incorporated with cellular proteins and utilized as chemical reporter for the profiling of acetylated proteins [1].
Reversible protein acetylation is generally regulated by lysine acetyltransferases (KATs) and lysine deacetylases (KDACs), which play important roles in regulating gene expression and occurrence of disease [1].
The p300-KAT-catalyzed acetylation with histone protein H3 showed that the the 4-pentynoate group can be efficiently transferred onto lysine residue. In jurkat cells, metabolic labeling of sodium 4-pentynoate was in time and dose dependent, which was optimal at 2.5-10 μM of acetate analogs for 6-8 hr. Fluorescent profiling identified 194 of 4-pentynoate labeled proteins from jurkat cells, where 86% of them were identified by radioactive method and 14% was newly identified [1].
Reference:
[1]. Yang Y Y, et al. Bioorthogonal chemical reporters for monitoring protein acetylation. J Am Chem Soc., 2010, 132(11): 3640-3641.
Cas No. | 101917-30-0 | SDF | |
别名 | 4-戊炔酸钠 | ||
化学名 | sodium pent-4-ynoate | ||
Canonical SMILES | C#CCCC(=O)[O-].[Na+] | ||
分子式 | C5H5NaO2 | 分子量 | 120.08 |
溶解度 | H2O : 100 mg/mL (832.78 mM; Need ultrasonic); DMSO : 16.67 mg/mL (138.82 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 8.3278 mL | 41.6389 mL | 83.2778 mL |
5 mM | 1.6656 mL | 8.3278 mL | 16.6556 mL |
10 mM | 0.8328 mL | 4.1639 mL | 8.3278 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。