Sodium copper chlorophyllin
(Synonyms: 叶绿素铜钠) 目录号 : GC32192
叶绿素铜钠对流感病毒和 HIV 具有抗病毒活性，IC50 为 50 至 100 μM 两者。
Cas No.:28302-36-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | MT-2 cells are pre-incubated to the bamboo leaf extract solution (up to 10%), Sodium copper chlorophyllin (up to 5 mM) or test reagent-free culture medium for 10 min to assess the inhibitory activity at adsorption. The pre-incubated cells are washed extensively to be able to eliminate antiviral activity of test reagents remaining in the culture medium, and then they are exposed to HIV-1LAI (1.3×10-4 TCID50 per cell). In addition to these pre-incubated cells, control wells are prepared to evaluate growth of the cells in a virus-free condition. Then, virus-exposed and mock-exposed cells are cultured in the absence of test reagents for three days. The total viable cells are counted on day 3[1]. |
References: [1]. Akiko Ito, et al. In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf Extract Solution and Sodium Copper Chlorophyllin. Yonago Acta Med. 2016 Mar; 59(1): 61-65. | |
Sodium copper chlorophyllin exerts antiviral activities against Influenza virus and HIV with IC50s of 50 to 100 μM for both of them.
Sodium copper chlorophyllin exhibits influenza virus adsorption at 200 µM (P<0.05). The 50% inhibitory concentration (IC50) and the 50% cytotoxic concentration (CC50) of Sodium copper chlorophyllin lay from 50 to 100 µM and 200 to 400 µM, respectively. Sodium copper chlorophyllin also inhibits HIV adsorption at 2.5 mM (P<0.05). The IC50 and CC50 of Sodium copper chlorophyllin lay from 50 to 100 µM and 200 to 400 µM, respectively[1].
[1]. Akiko Ito, et al. In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf Extract Solution and Sodium Copper Chlorophyllin. Yonago Acta Med. 2016 Mar; 59(1): 61-65.
| Cas No. | 28302-36-5 | SDF | |
| 别名 | 叶绿素铜钠 | ||
| Canonical SMILES | [O-]C(CC1=C(C(C([O-])=O)=C(C)C2=CC3=[N]4C(C(C=O)=C3CC)=CC5=C(C=C)C(C)=C6[N-]75)[N-]2[Cu+2]47[N](C8=C6)=C1C(C8C)CCC([O-])=O)=O.[Na+].[Na+].[Na+] | ||
| 分子式 | C34H29CuN4Na3O7 | 分子量 | 738.13 |
| 溶解度 | Water : 10 mg/mL (13.55 mM);DMSO : 6.67 mg/mL (9.04 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3548 mL | 6.7739 mL | 13.5477 mL |
| 5 mM | 0.271 mL | 1.3548 mL | 2.7095 mL |
| 10 mM | 0.1355 mL | 0.6774 mL | 1.3548 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Sodium copper chlorophyllin attenuates adenine-induced chronic kidney disease via suppression of TGF-beta and inflammatory cytokines
Naunyn Schmiedebergs Arch Pharmacol 2020 Nov;393(11):2029-2041.PMID:32500189DOI:10.1007/s00210-020-01912-3.
The present study was designed to evaluate the effect of Sodium copper chlorophyllin (SCC) in adenine-induced chronic kidney disease (CKD). CKD was induced in male Wistar rats by feeding 0.3% w/w adenine diet for 28 days. After induction, animals were treated with Sodium copper chlorophyllin at dose 2.7, 5.4, and 10.8 mg/kg for the next 28 days. The biochemical and urines parameters like creatinine, blood urea nitrogen (BUN), albumin, total protein creatinine clearance, urea clearance, and glomerular filtration rate were assessed on days 0, 14, and 28. Plasma TGF-尾1, COX-2, and IL-6 levels were assessed. Various oxidative stress parameters and TGF-尾1 expression were determined in the kidney. Histopathology of the kidney was studied with different stains. Sodium copper chlorophyllin-treated animals showed a significant reduction in urine output and relative kidney weight. The treatment with Sodium copper chlorophyllin significantly improved kidney function by normalizing biochemical and urine parameters. Treatment with SCC significantly reduced circulatory inflammatory mediators-TGF-尾1, COX-2, and IL-6. Additionally, the treatment also significantly reduced oxidative stress and TGF-尾1 expression in kidney tissues. Histopathology studies showed inhibition in the kidney damage due to the treatment of SCC. The Sodium copper chlorophyllin treatment attenuated adenine-induced chronic kidney disease in rats.
Simultaneous determination of sodium iron chlorophyllin and Sodium copper chlorophyllin in food using high-performance liquid chromatography and ultra-performance liquid chromatography-mass spectrometry
Food Chem 2019 Mar 15;276:390-396.PMID:30409610DOI:10.1016/j.foodchem.2018.10.015.
A simultaneous method for analyzing sodium iron chlorophyllin (SIC) and Sodium copper chlorophyllin (SCC) using high-performance liquid chromatography was developed. This method employed an Inertsil ODS-2 column and diode array detection at 395 nm, using methanol-water (97:3 and 80:20, v/v) containing 1% acetic acid as the mobile phase. Liquid chromatography-tandem mass spectrometry was used to identify the main components of SIC and SCC as Fe-isochlorine e4 and Cu-isochlorine e4, respectively. The limits of detection and quantitation of SIC were 1.2 and 4.1 mg/kg, respectively, while those of SCC were 1.4 and 4.8 mg/kg, respectively. For intraday and interday tests, the SIC recoveries from candy ranged from 81% to 101%, while SCC recoveries ranged from 100% to 109%. The developed method can be applied to the rapid determination of SIC and SCC in candy.
Sodium copper chlorophyllin Catalyzed Chemoselective Oxidation of Benzylic Alcohols and Diarylmethanes in Water
Molecules 2018 Jul 27;23(8):1883.PMID:30060516DOI:10.3390/molecules23081883.
We report the highly efficient and chemoselective oxidation of benzylic alcohols catalyzed by Sodium copper chlorophyllin in water, producing corresponding arylcarbonyl compounds. Importantly, the catalytic system exhibits a wide substrate scope and high functional group tolerance. Moreover, secondary alcohols and even diarylmethanes were smoothly oxidized to the desired aryl ketones with excellent yields.
Effects of Sodium Chlorophyllin Copper on APO-1 Expression in Bone Marrow Mesenchymal Stem Cells of Rats with Aplastic Anaemia
J Immunol Res 2022 Apr 6;2022:6792866.PMID:35434141DOI:10.1155/2022/6792866.
Background: Aplastic anaemia (AA) is a highly prevalent blood disorder in the East and Southeast Asian countries, and a proportion of the patients is poorly treated with immunosuppressive agents. This study is aimed at exploring the effects of Sodium copper chlorophyllin (SCC) on rats with AA and at providing the theoretical basis for the treatment of AA using traditional Chinese medicine. Methods: A rat model of AA was induced by combining 5-fluorouracil with busulfan, and different groups were treated with 25 mg/kg cyclosporin A (CsA) and low-, medium-, and high-dose SCC (25-, 50-, and 100-mg/kg; L-, M-, and H-SCC, respectively). A comparative analysis of peripheral blood counts, T-cell subsets, cytokine levels, bone marrow pathology, and APO-1 expression in mesenchymal stem cells in each group was conducted. Results: SCC can increase the platelet count and haemoglobin concentration in the peripheral blood of AA rats, whereas bone marrow biopsies revealed that the number of nucleated cells and megakaryocytes of SCC-treated rats increased compared with the model group. This was particularly evident in the H-SCC group. As regards the correction of immune function, unlike CsA, which reduced the absolute CD8+ T-cell count, SCC corrected the imbalanced CD4/CD8 ratio by increasing the absolute CD4+ T-cell count, whereas SCC increased the number of regulatory T-cells and reduced the level of interferon-纬 in AA rats. When comparing the expression of APO-1 in the MSCs, results of the reverse-transcriptase polymerase chain reaction and Western blot analysis showed that SCC can increase the expression of APO-1 both at the mRNA and protein levels. Conclusion: We found that SCC can improve haematopoietic function and regress immune disorders in AA rats, which enhanced the expression of APO-1 in bone marrow MSCs. This may be one of the mechanisms of SCC in treating AA.
In vitro inhibition of hyaluronidase by Sodium copper chlorophyllin complex and chlorophyllin analogs
Clin Cosmet Investig Dermatol 2015 Aug 12;8:443-8.PMID:26300653DOI:10.2147/CCID.S86863.
Background: Inhibitors of hyaluronidase are potent agents that maintain hyaluronic acid homeostasis and may serve as anti-aging, anti-inflammatory, and anti-microbial agents. Sodium copper chlorophyllin complex is being used therapeutically as a component in anti-aging cosmeceuticals, and has been shown to have anti-hyaluronidase activity. In this study we evaluated various commercial lots of Sodium copper chlorophyllin complex to identify the primary small molecule constituents, and to test various Sodium copper chlorophyllin complexes and their small molecule analog compounds for hyaluronidase inhibitory activity in vitro. Ascorbate analogs were tested in combination with copper chlorophyllin complexes for potential additive or synergistic activity. Materials and methods: For hyaluronidase activity assays, dilutions of test materials were evaluated for hydrolytic activity of hyaluronidase by precipitation of non-digested hyaluronate by measuring related turbidity at 595 nm. High-performance liquid chromatography and mass spectroscopy was used to analyze and identify the primary small molecule constituents in various old and new commercial lots of Sodium copper chlorophyllin complex. Results: The most active small molecule component of Sodium copper chlorophyllin complex was disodium copper isochlorin e4, followed by oxidized disodium copper isochlorin e4. Sodium copper chlorophyllin complex and copper isochlorin e4 disodium salt had hyaluronidase inhibitory activity down to 10 碌g/mL. The oxidized form of copper isochlorin e4 disodium salt had substantial hyaluronidase inhibitory activity at 100 碌g/mL but not at 10 碌g/mL. Ascorbate derivatives did not enhance the hyaluronidase inhibitory activity of Sodium copper chlorophyllin. Copper isochlorin e4 analogs were always the dominant components of the small molecule content of the commercial lots tested; oxidized copper isochlorin e4 was found in increased concentrations in older compared to newer lots tested. Conclusion: These results support the concept of using the hyaluronidase inhibitory activity of Sodium copper chlorophyllin complex to increase the hyaluronic acid level of the dermal extracellular matrix for the improvement of the appearance of aging facial skin.