Sophoricoside
(Synonyms: 槐角苷) 目录号 : GC31781
An isoflavone glycoside with diverse biological activities
Cas No.:152-95-4
Sample solution is provided at 25 µL, 10mM.
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Sophoricoside is a polyketide synthase-derived isoflavone glycoside that has been found in S. japonica and has diverse biological activities.1,2,3,4,5 It inhibits lipid accumulation induced by oleic acid in HepG2 cells and stimulates glucose uptake in C2C12 mouse skeletal muscle myoblasts when used at a concentration of 10 ?M.2 Sophoricoside (2 mg/kg) reduces scratching behavior induced by compound 48/80 or histamine in mice, as well as reduces serum levels of IgE in a mouse model of atopic dermatitis.3 It increases serum levels of osteocalcin and alkaline phosphatase (ALP) and restores mechanical bone hardness in an ovariectomized rat model of osteoporosis.4 Sophoricoside (80 and 160 mg/kg) decreases hepatic cholesterol and triglyceride levels, serum LDL and apolipoprotein B levels, and hepatic injury in a mouse model of fructose-induced liver injury.5
1.Trantas, E.A., Koffas, M.A.G., Xu, P., et al.When plants produce not enough or at all: Metabolic engineering of flavonoids in microbial hostsFront. Plant Sci.6(7)(2015) 2.Wu, C., Luan, H., Wang, S., et al.Modulation of lipogenesis and glucose consumption in HepG2 cells and C2C12 myotubes by sophoricosideMolecules18(12)15624-15635(2013) 3.Kim, S.-J., Lee, G.-Y., Jung, J.-W., et al.The ameliorative effect of sophoricoside on mast cell-mediated allergic inflammation in vivo and in vitroMolecules18(5)6113-6127(2013) 4.Abdallah, H.M., Al-Abd, A.M., Asaad, G.F., et al.Isolation of antiosteoporotic compounds from seeds of Sophora japonicaPLoS One9(6)e98559(2014) 5.Li, W., and Lu, Y.Hepatoprotective effects of sophoricoside against fructose-induced liver injury via regulating lipid metabolism, oxidation, and inflammation in miceJ. Food Sci.83(2)552-558(2018)
Kinase experiment: | Cells are incubated with the Sophoricoside for 1 h, and then incubated with PMACI for 2 h. The reaction is stopped by cooling the tubes in ice. The cells are separated from the released histamine by centrifugation at 400×g for 5 min at 4°C. The histamine levels are measured by ELISA using a histamine assay kit according to the manufacturer’s directions. Duplicate aliquots of supernatant are measured for each sample[1]. |
Cell experiment: | To test the cell viability by each concentration of Sophoricoside, the MTT colorimetric assay is performed. Briefly, HMC-1 cells (3×105 cells/well) are incubated with Sophoricoside (1 to 50 μM) for 12 h. After the addition of MTT solution, the cells are incubated at 37°C for 4 h. The crystallized MTT (formazan) is dissolved in dimethyl sulfoxide and the absorbance is measured at 540 nm[1]. |
Animal experiment: | BALB/c mice (5 weeks, 19 to 20 g) are used in this study. The dorsal skin of the BALB/c mice (n=6) is shaved and treated with a depilatory prior to the experiment. The mice are sensitized with 100 μL of 0.15% DNCB in acetone-olive oil (3:1) applied to the dorsal skin twice per week for 5 weeks. Control mice receive vehicle (acetone/olive oil=3:1). After 3 weeks, Sophoricoside (2 mg/kg) is orally administered 2 weeks until the end of the experiment[1]. |
References: [1]. Kim SJ, et al. The ameliorative effect of sophoricoside on mast cell-mediated allergic inflammation in vivo and in vitro. Molecules. 2013 May 22;18(5):6113-27. | |
| Cas No. | 152-95-4 | SDF | |
| 别名 | 槐角苷 | ||
| Canonical SMILES | OC1=CC(O)=C(C(C(C2=CC=C(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)C=C2)=CO4)=O)C4=C1 | ||
| 分子式 | C21H20O10 | 分子量 | 432.38 |
| 溶解度 | DMSO : ≥ 150 mg/mL (346.92 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3128 mL | 11.5639 mL | 23.1278 mL |
| 5 mM | 0.4626 mL | 2.3128 mL | 4.6256 mL |
| 10 mM | 0.2313 mL | 1.1564 mL | 2.3128 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet