Spiroxamine
(Synonyms: 螺噁茂胺) 目录号 : GC41258
A fungicide
Cas No.:118134-30-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Spiroxamine is a tertiary amine fungicide and an inhibitor of δ14 reductase/δ8→δ7 isomerase. It inhibits the growth of N. parvum, B. dothidea, D. seriata, and L. theobromae isolates from grape vines (EC50s = 0.97-10.28 mg/L). Spiroxamine (0.03-30 μM) reduces network formation in rat cortical cultures. It is also cytotoxic to MDA-kb2 cells (EC20 = 9.29 μM).
| Cas No. | 118134-30-8 | SDF | |
| 别名 | 螺噁茂胺 | ||
| Canonical SMILES | CCN(CC1COC2(CCC(C(C)(C)C)CC2)O1)CCC | ||
| 分子式 | C18H35NO2 | 分子量 | 297.5 |
| 溶解度 | DMSO : ≥ 250 mg/mL (840.39 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3613 mL | 16.8067 mL | 33.6134 mL |
| 5 mM | 0.6723 mL | 3.3613 mL | 6.7227 mL |
| 10 mM | 0.3361 mL | 1.6807 mL | 3.3613 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Peer review of the pesticide risk assessment for the active substance Spiroxamine in light of confirmatory data submitted
EFSA J 2021 Feb 5;19(2):e06385.PMID:33598047DOI:10.2903/j.efsa.2021.6385.
The conclusions of the EFSA following the peer review of the initial risk assessment carried out by the competent authority of the rapporteur Member State, Germany, for the pesticide active substance Spiroxamine are reported. The context of the peer review was that requested by the European Commission following the submission and evaluation of confirmatory ecotoxicology data. The conclusions were reached on the basis of the evaluation of the representative uses of Spiroxamine as a fungicide on grapes, wheat, triticale, rye, barley and oats. The reliable end points concluded as being appropriate for use in regulatory risk assessment, derived from the available studies and literature in the dossier peer reviewed, are presented.
Residues, dissipation and risk evaluation of Spiroxamine in open-field-grown strawberries using liquid chromatography tandem mass spectrometry
Biomed Chromatogr 2020 Jul;34(7):e4836.PMID:32222076DOI:10.1002/bmc.4836.
The dissipation dynamic and residues of Spiroxamine in open-field-grown strawberries were determined using liquid chromatography tandem mass spectrometry (LC-MS/MS). Spiroxamine application was performed according to Egyptian good agricultural practices recommendation. A QuEChERS-based extraction method along with direct analysis with an LC-MS/MS analytical method were optimized and validated, and the specificity of the techniques used was considered satisfactory. Good linearity (R2 > 0.999) was obtained for Spiroxamine within the range of 0.001-0.1 μg/ml. The mean recoveries varied between 97.1 and 108.2%, with inter- and intra-day precision (RSD) <4.9%. The limit of quantitation for Spiroxamine was 0.001 mg/kg. The results indicated that Spiroxamine degradation in strawberry followed first-order kinetics (R2 > 0.9929) with an estimated half-life value of 4.71 days. Considering the Australian maximum residue limit (0.05 mg/kg) in strawberry and based on the results from residue trials with a preharvest interval of 14 days for strawberry, compliance can be expected. The present results could provide guidance to fully evaluate the risks of Spiroxamine residues, preventing any potential health risk to consumers.
Residues of Spiroxamine in grapes following field application and their fate from vine to wine
J Agric Food Chem 2005 Dec 28;53(26):10091-6.PMID:16366700DOI:10.1021/jf052162q.
Dissipation of the fungicide Spiroxamine in grapes of two vine varieties, Roditis and Cabernet Sauvignon, exposed to field treatments was evaluated. Vines of a grape vineyard located in central Greece were sprayed once or twice with a commercial formulation of the fungicide at 30 g a.i./hL. Residues in grapes, must, and wine were determined by gas chromatography/IT-MS after extraction with cyclohexane-dichloromethane (9:1), with a limit of quantitation 0.02 mg/kg in grapes and 0.012 mg/kg in wine. Under field conditions, Spiroxamine dissipation on grapes was faster during the first 2 weeks and then slower to the sixth week. About 7 days after application, half of the initial Spiroxamine concentration remained on the grapes; the respective proportion at 42 days was about 10%. At 14 and 35 days, residues were lower than 0.44 and 0.22 mg/kg, respectively, values below the maximum residue levels set by the European Union (1 mg/kg). Spiroxamine residues transferred from grapes into the must and through the vinification process into the wine were also studied. Mean transfer factors of 0.26 and 0.55 were found from grapes into wine for the wines obtained without maceration and with maceration, respectively. Residues in wine, prepared from grapes with a Spiroxamine content of 0.11-0.20 mg/kg, varied from <0.026 to 0.09 mg/kg. Spiroxamine diastereomer B was found to dissipate slower than diastereomer A in the field as well as during the vinification process.
Hydrolysis study and extraction of Spiroxamine from soils of different physico-chemical properties
Chemosphere 2009 Oct;77(6):821-8.PMID:19733892DOI:10.1016/j.chemosphere.2009.07.078.
Spiroxamine, 8-(1,1-dimethylethyl)-N-ethyl-N-propyl-1,4-dioxa-spiro [4.5] decane-2-methanamine, is a novel broad-spectrum fungicide from the new class of spiroketalamines widely used in cereals and grapes. In this paper, the hydrolysis of the Spiroxamine active substance has been investigated and the extraction procedure from soil samples has been optimized. The progress of degradation in water at a wide pH range (4-14), was continuously monitored by means of reversed phase LC-ESI-MS/MS in the positive ionization mode during 90 d incubation experiment. To establish the degradation kinetics, water solutions were incubated by triplicate in the dark under controlled temperature (20+/-2 degrees C) and the Spiroxamine remaining concentration was determined. Pesticide metabolites such as desethyl, despropyl and N-oxide Spiroxamine were not detected at significant amounts in all tested conditions and the degradations followed first-order kinetics (r(2)>or=0.979). The faster degradation was observed at both pH 4 and 14 with half-life times of 37 and 10 d, respectively. On the other hand, the Spiroxamine fungicide has been determined in two types of soil (silt loam and sandy loam) at different spiked levels. At the level of the highest Spiroxamine application rate for agricultural crops (750 g a.i. ha(-1)), recoveries higher than 96.0% were obtained, showing the effectiveness of the proposed methodology.
Dissipation and metabolism of (14)C-spiroxamine in soil under laboratory condition
Environ Pollut 2010 May;158(5):1542-50.PMID:20060629DOI:10.1016/j.envpol.2009.12.025.
Spiroxamine [SPX] belongs to a spiroketalamine group of substances. The biodegradation of [1,3-dioxolane-4-(14)C]-SPX has been examined in 2 soils of different physicochemical properties. The total recovery of radioactivity from soils was 98.6-103.5% of that applied. The total amount of extractable radioactivity declined with a simultaneous increase in non-extractable radioactivity. Volatile organics were detected at lower levels; however, mineralization played a marked effect on the route of SPX dissipation. The half-life ranges between 37 and 44 d. SPX does not undergo any enantioselective degradation. 4 metabolites: despropyl-SPX, desethyl-SPX, SPX N-oxide and SPX acid were identified, applying mass spectrometric technique. Sorption-desorption data fitted well with a Freundlich model in log form (r(2), 0.99). K(Dsorp) ranged between 44 and 230, suggesting SPX ought to be considered as a substance with low leaching potential [groundwater ubiquity score (GUS), <1.8]. Furthermore, an overall low desorption of 1-11% indicates firm retention of SPX by the soils.