Stachydrine (hydrochloride)
(Synonyms: 盐酸水苏碱) 目录号 : GC13840
A pyrrolidine betaine with anticancer and cardioprotective effects
Cas No.:4136-37-2
Sample solution is provided at 25 µL, 10mM.
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Stachydrine is an anti-metastatic agent.
Stachydrine, a pyrrolidine betaine, was first isolated from seed husk and the pulp of the fruit of C. Leonurus, and many other Asian plants and fruits.
In vitro: The aim of a previous study was to investigate the effects of stachydrine (STA) on norepinephrine (NE) induced hypertrophy and the changes of calcium transients in neonatal rat cardiomyocytes. Results showed that NE treatment could increase protein synthesis, cell surface area, the expression level of β-MHC and β/α-MHC ratio and these effects were attenuated by STA treatment. In addition, NE-induced hypertrophy was associated with accelerated decay of Ca2+-transient, increased phospholamban expression, increased Ca2+-transient amplitude, hyper-phosphorylation, increased intracellular cAMP level, as well as PKA overactivation. All of these effects could be inhibited by STA treatment significantly [1].
In vivo: Previous study evaluated the effect of stachydrine on the expression of caspase-9 and 12 in rats with unilateral ureteral obstruction. Results showed that degree of renal interstitial fibrosis, serum creatinine, blood urea nitrogen, the renal tubular interstitial damage index, and expression of caspase-9 and 12 in the stachydrine-treatment groups were significantly decreased [2].
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Zhang, C. ,Shan, X.-L.,Liao, Y.-L., et al. Effects of stachydrine on norepinephrine-induced neonatal rat cardiac myocytes hypertrophy and intracellular calcium transients. BMC Complement. Altern. Med. 14, 474 (2014).
[2] Zhang C, Lu Y, Zhou YJ, Tong QQ, Qu C, Kang TJ. The effect of stachydrine on the expression of caspase-12 in rats with unilateral ureteral obstruction. J Urol. 2014 Nov;192(5):1549-54.
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Cell experiment: |
Cell Proliferation Assay[2]Cell Cycle Analysis[2]MCF-7 and T47D cells50 µM, 200 µM, 500 µM and 1000 µM24 and 48 hoursCCK-8 cell counting assay is used to detect proliferation of MCF-7 and T47D cells when treated with Stachydrine hydrochloride at four different concentrations (50 µM, 200 µM, 500 µM and 1000 µM) for 24 and 48 hours. Cell cycle analysis of MCF-7 and T47D cells is determined by flow cytometry. Experiments are performed in triplicates and representative images are presented. Note: Inhibited proliferation and induced G1 phase arrest in MCF-7 and T47D cells. |
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References: [1]. Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. |
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| Cas No. | 4136-37-2 | SDF | |
| 别名 | 盐酸水苏碱 | ||
| 化学名 | (2S)-2-carboxy-1,1-dimethyl-pyrrolidinium, inner salt, monohydrochloride | ||
| Canonical SMILES | C[N+]1(C)CCC[C@H]1C([O-])=O.Cl | ||
| 分子式 | C7H13NO2.HCl | 分子量 | 179.6 |
| 溶解度 | ≤10mg/ml in ethanol;15mg/ml in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.5679 mL | 27.8396 mL | 55.6793 mL |
| 5 mM | 1.1136 mL | 5.5679 mL | 11.1359 mL |
| 10 mM | 0.5568 mL | 2.784 mL | 5.5679 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet