Stercobilin (hydrochloride)
(Synonyms: (–)-Stercobilin, L-Stercobilin) 目录号 : GC49844
A fecal pigment and metabolite of bilirubin
Cas No.:5370-34-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Stercobilin is a fecal pigment and a metabolite of the heme catabolite bilirubin .1 It induces DNA damage in a comet assay in HepG2 cells when used at concentrations ranging from 0.5 to 17 µM.2 Stercobilin (20 and 30 µM) induces activation of NF-κB in a reporter assay and increases the expression of the genes encoding TNF-α, IL-1β, IL-6, inducible nitric oxide synthase (iNOS), and COX-2 in RAW 264.7 macrophages.3 Fecal and plasma levels of stercobilin are increased in ob/ob mice.
1.VÍtek, L., Majer, F., MuchovÁ, L., et al.Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal floraJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.833(2)149-157(2006) 2.MÖlzer, C., Pfleger, B., Putz, E., et al.In vitro DNA-damaging effects of intestinal and related tetrapyrroles in human cancer cellsExp. Cell Res.319(4)536-545(2013) 3.Sanada, S., Suzuki, T., Nagata, A., et al.Intestinal microbial metabolite stercobilin involvement in the chronic inflammation of ob/ob miceSci. Rep.10(1)6479(2020)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6812 mL | 8.4062 mL | 16.8124 mL |
| 5 mM | 0.3362 mL | 1.6812 mL | 3.3625 mL |
| 10 mM | 0.1681 mL | 0.8406 mL | 1.6812 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。