Steryl Glucosides
目录号 : GC40014
A mixture of steryl glucosides
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Steryl glucosides are neutral glycolipids commonly found in plant cell membranes and vegetable oils that contain a glucose moiety conjugated to a sterol lipid. They function as glucose donors in the biosynthesis of glucocerebrosides in plant microsomes and are metabolic precursors to acylated/esterified steryl glucosides. Steryl glucosides are the major component of filter- and engine-damaging precipitates formed during biodiesel production from transesterification of vegetable oils. This product contains a mixture of steryl glucosides.
| Cas No. | SDF | ||
| 分子式 | C35H60O6 (for ǂ-sitosteryl glucoside) | 分子量 | 576.9 |
| 溶解度 | Chloroform:Methanol:Water (2:1:0.1): soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7334 mL | 8.667 mL | 17.334 mL |
| 5 mM | 0.3467 mL | 1.7334 mL | 3.4668 mL |
| 10 mM | 0.1733 mL | 0.8667 mL | 1.7334 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Analysis of Steryl Glucosides in rice bran-based fermented food by LC/ESI-MS/MS
Steroids 2020 Jun;158:108605.PMID:32084504DOI:10.1016/j.steroids.2020.108605.
Steryl Glucosides (SGs) and acylated Steryl Glucosides (ASGs) are phytochemicals found in plant-based foods and are known as bioactive compounds with potential health benefits. These include anti-inflammatory properties, anti-diabetic effects, and modulation of immunoregulatory functions as well as having cholesterol lowering effects. In this study, three major SGs, i.e., glucosides of β-sitosterol, stigmasterol, and campesterol, were synthesized and used as standards for measurement of their contents in rice bran (RB)-based fermented food (FBRA) utilizing Aspergillus oryzae and raw material (RM). The compounds were quantified using liquid chromatography/electrospray ionization-tandem mass spectrometry. It was found that β-sitosteryl glucoside was most abundant among the analyzed glucosides in both samples, and the contents of each SG in FBRA decreased about 35% from those of RM. In contrast to SGs, the contents of ASGs in FBRA increased 1.5-fold during the fermentation process as evidenced by an alkaline hydrolysis. The present results suggest that the FBRA might have greater beneficial effects than the RM, since ASGs have shown to have more potent cholesterol lowering effects and stronger anti-diabetic properties than SGs.
Cloning and Production of Thermostable Enzymes for the Hydrolysis of Steryl Glucosides in Biodiesel
Methods Mol Biol 2021;2290:203-214.PMID:34009592DOI:10.1007/978-1-0716-1323-8_14.
Vegetable oil-derived biodiesels have a major quality problem due to the presence of precipitates formed by Steryl Glucosides, which clog filters and injectors of diesel engines. An efficient, scalable, and cost-effective method to hydrolyze Steryl Glucosides using thermostable enzymes has been developed. Here, methods to discover, express in recombinant microorganisms and manufacture enzymes with SGase activity, as well as methods to treat biodiesel with such enzymes, and to measure the content of Steryl Glucosides in biodiesel samples are presented.
The production, properties, and applications of thermostable steryl glucosidases
World J Microbiol Biotechnol 2018 Feb 21;34(3):40.PMID:29468428DOI:10.1007/s11274-018-2423-x.
Extremophilic microorganisms are a rich source of enzymes, the enzymes which can serve as industrial catalysts that can withstand harsh processing conditions. An example is thermostable β-glucosidases that are addressing a challenging problem in the biodiesel industry: removing Steryl Glucosides (SGs) from biodiesel. Steryl glucosidases (SGases) must be tolerant to heat and solvents in order to function efficiently in biodiesel. The amphipathic nature of SGs also requires enzymes with an affinity for water/solvent interfaces in order to achieve efficient hydrolysis. Additionally, the development of an enzymatic process involving a commodity such as soybean biodiesel must be cost-effective, necessitating an efficient manufacturing process for SGases. This review summarizes the identification of microbial SGases and their applications, discusses biodiesel refining processes and the development of analytical methods for identifying and quantifying SGs in foods and biodiesel, and considers technologies for strain engineering and process optimization for the heterologous production of a SGase from Thermococcus litoralis. All of these technologies might be used for the production of other thermostable enzymes. Structural features of SGases and the feasibility of protein engineering for novel applications are explored.
Biosynthesis and structure of glycosyl diglycerides, Steryl Glucosides, and acylated Steryl Glucosides
Lipids 1975 Jul;10(7):427-36.PMID:167261DOI:10.1007/BF02532449.
A particulate enzyme fraction from Mycobacterim smegmatis catalyzed the transfer of -14C-glucose from the UDP--14C-glucose into neutral glycolipids. The two major radioactive components were purified by column chromatography on O-diethylamino ethyl cellulose (acetate) and thin layer chromatography on silica gel in several solvents. The first product yielded a water-soluble component upon saponification, which had a hexoseglycerol ratio of 1:1 with all of the hexose being identified as glucose. The second product yielded a water-soluble component upon saponification which contained hexose and glycerol in a 2:1 ratio and, in addition to glucose, contained lesser amounts of mannose and galactose. Palmitate and oleate were the predominant fatty acids and were present in equimolar amounts. The products thus have been identified as monoglycosyldiglyceride and diglycosyldiglyceride. The diglycosyldiglyceride could also be labeled with -14C-galactose when UDP--14C-galactose served as the donor, but the monoglycosyldiglyceride was only slightly labeled with -14C-galactose. Membrane fractions from mung bean seedlings catalyzed the transfer of -14C-glucose from UDP--14C-glucose into a neutral glycolipid which has been purified by thin layer chromatography and analyzed by combined gas liquid chromatography-mass spectrometry. It was determined to be a steryl glucoside with the two major sterol components being betasitosterol and stigmasterol linked to beta-D-glucose. Particulate fractions from developing cotton fibers also catalyzed the formation of Steryl Glucosides and, in addition, they catalyzed the esterification of Steryl Glucosides at the 6 position of glucose with fatty acids (primarily palmitate and oleate) from an andogenous acyl donor. Both the glucosyl transferase and the acyltransferase have been solubilized with Triton X-100 and partially purified by chromatography on Sephadex G-200. The acyltransferase activity was reconstituted by the addition of the steryl glucoside and a phospholipid acyl donor.
The biosynthesis of Steryl Glucosides in plants
Plant Physiol 1970 Mar;45(3):255-62.PMID:5423466DOI:10.1104/pp.45.3.255.
Mitochondrial preparations from pea root (Pisum sativum L. var. Alaska) cauliflower inflorescence (Brassica cauliflora Gars.) and avocado inner mesocarp (Persea americana Mill. var. Fuerte), and chloroplast preparations from spinach leaf (Spinacia oleracea L. var. Bloomsdale) incorporate glucose into steryl glucoside and acylated steryl glucoside when either uridine diphosphate-glucose or uridine diphosphate-galactose is supplied as precursor. In the case of pea root mitochondria, galactosyl diglycerides are not formed from either nucleotide sugar. In the case of spinach chloroplasts only 3% of the metabolized uridine diphosphate-galactose is found as steryl glycosides. Time course experiments indicate that the steryl glucoside is the precursor of the acylated steryl glucoside. The effect of pH on the over-all reaction and analysis of the reaction products suggest that the glucosylation of the sterol has a pH optimum of 8 to 9, and the pH optimum for the acylation of the steryl glucoside is 6.5 to 7. The synthesis of steryl glucoside and acylated steryl glucoside, catalyzed by acetone powders of pea root mitochondria, is stimuated by added sitosterol and stigmasterol.