Stigmasterol
(Synonyms: 豆甾醇; Stigmasterin) 目录号 : GN10359
Stigmasterol豆甾醇是一种天然的甾醇。Stigmasterol具有显著的抗关节炎和抗炎作用。
Cas No.:83-48-7
Sample solution is provided at 25 µL, 10mM.
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Stigmasterol is a naturally available sterol. stigmasterol has significant anti-arthritis and anti-inflammatory effects[1-3].
Stigmasterol(0-100μM;14 h) inhibited glutamate-induced neuronal cell death in the HT-22 cells[4].Stigmasterol(0-20μM;4 h) alleviated Aβ42 oligomers induced inflammatory response in BV2 cells[5].
Stigmasterol(400 mg/kg/d; i. g; 10 days) treatment attenuates DSS-induced colitis in mice[6].Stigmasterol(100 mg/kg;i.p;21-27days) inhibited inflammation and oxidative stress in a mouse model of asthma by NK1-R[7].In the hippocampus of APP/PS1 mice, stigmasterol suppressed the microglia activation[8], and stigmasterol also decreased the TNF-α levels in the cortex of Swiss albino mice[9].
References:
[1]. Antwi AO, Obiri DD, et,al. Stigmasterol Alleviates Cutaneous Allergic Responses in Rodents. Biomed Res Int. 2018 Jul 24;2018:3984068. doi: 10.1155/2018/3984068. PMID: 30140696; PMCID: PMC6081592.
[2]. Gabay O, Sanchez C, et,al. Stigmasterol: a phytosterol with potential anti-osteoarthritic properties. Osteoarthritis Cartilage. 2010 Jan;18(1):106-16. doi: 10.1016/j.joca.2009.08.019. Epub 2009 Sep 15. PMID: 19786147.
[3]. Chen WP, Yu C, et,al. Stigmasterol blocks cartilage degradation in rabbit model of osteoarthritis. Acta Biochim Pol. 2012;59(4):537-41. Epub 2012 Oct 16. PMID: 23074702.
[4]. Mongkolpobsin K, Sillapachaiyaporn C, et,al. Stigmasterol isolated from Azadirachta indica flowers attenuated glutamate-induced neurotoxicity via downregulation of the Cdk5/p35/p25 signaling pathway in the HT-22 cells. Phytomedicine. 2023 May;113:154728. doi: 10.1016/j.phymed.2023.154728. Epub 2023 Feb 24. PMID: 36898255.
[5]. Jie F, Yang X, et,al. Stigmasterol attenuates inflammatory response of microglia via NF-κB and NLRP3 signaling by AMPK activation. Biomed Pharmacother. 2022 Sep;153:113317. doi: 10.1016/j.biopha.2022.113317. Epub 2022 Jun 27. PMID: 35772378.
[6]. Wen S, He L, et,al. Stigmasterol Restores the Balance of Treg/Th17 Cells by Activating the Butyrate-PPARγ Axis in Colitis. Front Immunol. 2021 Oct 6;12:741934. doi: 10.3389/fimmu.2021.741934. PMID: 34691046; PMCID: PMC8526899.
[7]. Zhang J, Zhang C, et,al. Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor. Pharm Biol. 2023 Dec;61(1):449-458. doi: 10.1080/13880209.2023.2173252. PMID: 36788676; PMCID: PMC9930798.
[8]. Koivisto H, Grimm MO, et,al. Special lipid-based diets alleviate cognitive deficits in the APPswe/PS1dE9 transgenic mouse model of Alzheimer's disease independent of brain amyloid deposition. J Nutr Biochem. 2014 Feb;25(2):157-69. doi: 10.1016/j.jnutbio.2013.09.015. Epub 2013 Nov 6. PMID: 24445040.
[9]. Yadav M, Parle M, et,al. Protective effects of stigmasterol against ketamine-induced psychotic symptoms: Possible behavioral, biochemical and histopathological changes in mice. Pharmacol Rep. 2018 Jun;70(3):591-599. doi: 10.1016/j.pharep.2018.01.001. Epub 2018 Jan 11. PMID: 29679883.
Stigmasterol豆甾醇是一种天然的甾醇。Stigmasterol具有显著的抗关节炎和抗炎作用[1-3]。
Stigmasterol (0-100μM;14 h)抑制谷氨酸诱导的HT-22细胞死亡[4]。Stigmasterol (0-20μM;4 h)可减轻Aβ42低聚物诱导的BV2细胞炎症反应[5]。
Stigmasterol (400 mg/kg/d; i. g; 10 days) 治疗可减轻DSS诱导的小鼠结肠炎[6]。Stigmasterol (100 mg/kg;i.p;21-27days)通过NK1-R抑制小鼠哮喘模型的炎症和氧化应激[7]。在APP/PS1小鼠海马体中，Stigmasterol抑制了小胶质细胞的激活，并降低了Swiss albino小鼠皮质中TNF-α的水平[8-9]。
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Cell experiment [1]: |
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Cell lines |
Murine BV2 microglia cells |
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Preparation method |
Cells were treated with Aβ42 oligomers for 24 h, followed by treatment of stigmasterol (dissolved in ethanol) for 4 h. |
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Reaction Conditions |
0-20μM;4 h |
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Applications |
Stigmasterol alleviated Aβ42 oligomers induced inflammatory response in BV2 cells. |
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Animal experiment [2]: |
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Animal models |
Male C57bl/6J mice (ovalbumin (OVA)-induced asthma model) |
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Preparation method |
Asthma mice were divided into OVA group, Stigmasterol group, and Stigmasterol + rh-NK1R protein (Stig-NK1R) group. In corresponding groups, 100 mg/kg stigmasterol, and 100 μg rh-NK1R were intraperitoneally injected into mice on days 21, 22, 23, 24, 25, 26, 27 before the challenge with OVA. |
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Dosage form |
100 mg/kg;i.p;21-27days |
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Applications |
Stigmasterol inhibited inflammation and oxidative stress in a mouse model of asthma by NK1-R. |
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References: [1]. Jie F, Yang X,et,al. Stigmasterol attenuates inflammatory response of microglia via NF-κB and NLRP3 signaling by AMPK activation. Biomed Pharmacother. 2022 Sep;153:113317. doi: 10.1016/j.biopha.2022.113317. Epub 2022 Jun 27. PMID: 35772378. [2]. Zhang J, Zhang C, et,al. Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor. Pharm Biol. 2023 Dec;61(1):449-458. doi: 10.1080/13880209.2023.2173252. PMID: 36788676; PMCID: PMC9930798. |
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| Cas No. | 83-48-7 | SDF | |
| 别名 | 豆甾醇; Stigmasterin | ||
| 化学名 | (3S,8S,9S,10R,13R,14S,17R)-17-[(E,2R,5S)-5-ethyl-6-methylhept-3-en-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol | ||
| Canonical SMILES | CCC(C=CC(C)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)O)C)C)C(C)C | ||
| 分子式 | C29H48O | 分子量 | 412.69 |
| 溶解度 | 5mg/mL in Ethanol (Need ultrasonic) | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4231 mL | 12.1156 mL | 24.2313 mL |
| 5 mM | 0.4846 mL | 2.4231 mL | 4.8463 mL |
| 10 mM | 0.2423 mL | 1.2116 mL | 2.4231 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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