Succimer (Dimercaptosuccinic acid)
(Synonyms: 二巯基丁二酸; Dimercaptosuccinic acid; DMSA) 目录号 : GC30197
A heavy metal chelator
Cas No.:304-55-2
Sample solution is provided at 25 µL, 10mM.
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meso-2,3-Dimercaptosuccinic acid is a heavy metal chelator.1 It reverses sodium arsenite-induced inhibition of pyruvate dehydrogenase (PDH) in mouse kidney homogenates (EC50 = 0.25 mM). meso-2,3-Dimercaptosuccinic acid decreases cisplatin-induced increases in serum levels of platinum and blood urea nitrogen (BUN), as well as apoptosis in renal tubules of mice when administered at a dose of 100 ?M/kg.2 meso-2,3-Dimercaptosuccinic acid (90 mg/kg) decreases elevations in the activity of catalase and glucose-6-phosphate dehydrogenase (G6PDH) in RBCs from lead-exposed rats, and decreases the levels of lead in the cerebellum, hippocampus, liver, kidney, and blood in rats when administered at a dose of 100 mg/kg.3,4 It decreases immobility time in the forced swim test in lead-exposed male, but not female, mice when administered at a dose of 50 mg/kg.5 Formulations containing meso-2,3-dimercaptosuccinic acid have been used in the treatment of heavy metal poisoning.
1.Aposhian, H.V., Hsu, C.-A., and Hoover, T.D.DL- and meso-dimercaptosuccinic acid: in vitro and in vivo studies with sodium arseniteToxicol. Appl. Pharmacol.69(2)206-213(1983) 2.Yajima, Y., Kawaguchi, M., Yoshikawa, M., et al.The effects of 2,3-dimercapto-1-propanesulfonic acid (DMPS) and meso-2,3-dimercaptosuccinic acid (DMSA) on the nephrotoxicity in the mouse during repeated cisplatin (CDDP) treatmentsJ. Pharmacol. Sci.134(2)108-115(2017) 3.Gürer, H., ?zgünes, H., Neal, R., et al.Antioxidant effects of N-acetylcysteine and succimer in red blood cells from lead-exposed ratsToxicology128(3)181-189(1998) 4.Zhang, J., Wang, X.-F., Lu, Z.-B., et al.The effects of meso-2,3-dimercaptosuccinic acid and oligomeric procyanidins on acute lead neurotoxicity in rat hippocampusFree Rad. Biol. Med.37(7)1037-1050(2004) 5.Stewart, P.W., Blaine, C., Cohen, M., et al.Acute and longer term effects of meso-2,3 dimercaptosuccinic acid (DMSA) on the behavior of lead-exposed and control micePhysiol. Behav.59(4-5)849-855(1996)
Animal experiment: | All experiments are performed with Fisher 344 male rats weighing 75 to 100 g. The animals are randomized into four groups. Group I (n=11) serves as the control and is given only standard rat chow and water for 6 weeks. Group II (n=11) receives 2000 ppm lead acetate in its drinking water for 5 weeks and, during the 6th week, this group receives water. Group III (n=6) receives 2000 ppm lead acetate in its drinking water for 5 weeks and, during the 6th week, these animals receive 800 mg/kg/day NAC dissolved in water. Group IV (n=6) is treated like group III, except that it receives 90 mg/kg/day Succimer during the last week. At the end of the 6th week, after overnight fasting, the animals are anesthetized with metofane and blood samples are collected via intracardiac puncture using heparin as an anticoagulant. Plasma and the buffy coat are removed by centrifugation for 10 min at 3000 rpm. The RBCs are washed three times with an equal volume of cold saline[1]. |
References: [1]. Gürer H, et al. Antioxidant effects of N-acetylcysteine and succimer in red blood cells from lead-exposed rats. Toxicology. 1998 Jul 17;128(3):181-9. | |
| Cas No. | 304-55-2 | SDF | |
| 别名 | 二巯基丁二酸; Dimercaptosuccinic acid; DMSA | ||
| Canonical SMILES | O=C(O)[C@@H](S)[C@@H](S)C(O)=O | ||
| 分子式 | C4H6O4S2 | 分子量 | 182.22 |
| 溶解度 | DMSO : 300 mg/mL (1646.36 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.4879 mL | 27.4394 mL | 54.8787 mL |
| 5 mM | 1.0976 mL | 5.4879 mL | 10.9757 mL |
| 10 mM | 0.5488 mL | 2.7439 mL | 5.4879 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet